Expression of phosphinothricin N-acetyltransferase in Escherichia coli and Pseudomonas fluorescens: Influence of mRNA secondary structure, host, and other physiological conditions

Protein Expression and Purification

Volumn 55, Issue 2, 2007, Pages 352-360

  Madduri, Krishna M ^a   Snodderley, Erika M ^a  

^a DOW AGROSCIENCES LLC   (United States)

Author keywords

E. coli; In vitro; P. fluorescens; PAT

Indexed keywords

ACYLTRANSFERASE; MESSENGER RNA; PHOSPHINOTHRICIN N ACETYLTRANSFERASE; PHOSPHINOTHRICIN N-ACETYLTRANSFERASE; PRIMER DNA; UNCLASSIFIED DRUG;

AMINO ACID SEQUENCE; ARTICLE; CHEMISTRY; CONFORMATION; ESCHERICHIA COLI; GENETICS; METABOLISM; MOLECULAR GENETICS; NUCLEOTIDE SEQUENCE; OPEN READING FRAME; POLYACRYLAMIDE GEL ELECTROPHORESIS; PSEUDOMONAS FLUORESCENS; WESTERN BLOTTING;

ACETYLTRANSFERASES; AMINO ACID SEQUENCE; BASE SEQUENCE; BLOTTING, WESTERN; DNA PRIMERS; ELECTROPHORESIS, POLYACRYLAMIDE GEL; ESCHERICHIA COLI; MOLECULAR SEQUENCE DATA; NUCLEIC ACID CONFORMATION; OPEN READING FRAMES; PSEUDOMONAS FLUORESCENS; RNA, MESSENGER;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI; PSEUDOMONAS FLUORESCENS;

EID: 34548405165     PISSN: 10465928     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.pep.2007.04.026     Document Type: Article

References (20)

1. Wild A., and Manderscheid R. The effect of phosphinothricin on the assimilation of ammonia in plants. J. Biosci. 39 (1984) 500-504
2. Schwartz D., Berger S., Heinzelmann E., Muschko K., Welzel K., and Wohlleben W. Biosynthetic gene cluster of the herbicide phosphinothricin tripeptide from Streptomyces viridochromogenes Tü494. Appl. Environ. Microbiol. 70 (2004) 7093-7102
3. Strauch E., Wohlleben W., and Puehler A. Cloning of a phosphinothricin N-acetyltransferase gene from Streptomyces viridochromogenes Tu494 and its expression in Streptomyces lividans and Escherichia coli. Gene 63 (1988) 65-74
4. Wohlleben W., Arnold W., Broer I., Hillemann D., Strauch E., and Puehler A. Nucleotide sequence of the phosphinothricin N-acetyltransferase gene from Streptomyces viridochromogenes Tu494 and its expression in Nicotiana tabacum. Gene 70 (1988) 25-37
5. Rasche E., Cremer J., Donn G., and Zink J. The development of glufosinate ammonium tolerant crops in market. Br. Crop Prot. Con. Weeds 2 (1995) 415-426
6. Herouet C., Esdaile D.J., Mallyon B.A., Debruyne E., Schulz A., Currier T., Hendrickx K., van der Klis R.-J., and Rouan D. Safety evaluation of the phosphinothricin acetyltransferase proteins encoded by the pat and bar sequences that confer tolerance to glufosinate-ammonium herbicide in transgenic plants. Regul. Toxicol. Pharmacol. 41 (2005) 134-149
7. Yamaguchi H., Sasaki K., Umetsu H., and Kamada H. Two detection methods of genetically modified maize and the state of its import into Japan. Food Control 14 (2003) 201-206
8. D.V. Onisk, J.W. Stave, A.B. McQuillin, Reagents, method and kit for detecting phosphinothricin-N-acetyltransferase protein, PCT Int. Appl. (2002) 31 pp., WO 2002027322 A2 20020404.
9. Wehrmann A., Vliet V., Opsomer C., Botterman J., and Schulz A. The similarities of bar and pat gene products make them equally applicable for plant engineers. Nat. Biotechnol. 14 (1996) 1274-1278
10. Schneider J.C., Jenings A.F., Mun D.M., McGovern P.M., and Chew L.C. Auxotrophic markers pyrF and proC can replace antibiotic markers on protein production plasmids in high-cell-density Pseudomonas fluorescens fermentation. Biotechnol. Progr. 21 (2005) 343-348
11. P. Eckes, B. Uijtewaal, G. Donn, A synthetic gene confers resistance against the broad spectrum herbicide l-phosphinothricin in plants, J. Cell. Biochem. Abstract M516, 13D (1989), 334.
12. Enderle P.J., and Farwell M.A. Electroporation of freshly plated Escherichia coli and Pseudomonas aeruginosa cells. Biotechniques 25 (1998) 954-958
13. Retallack D.M., Thomas T.C., Shao Y., Haney K.L., Resnick S.M., Lee V.D., and Squires C.H. Identification of anthranilate and benzoate metabolic operons of Pseudomonas fluorescens and functional characterization of their promoter regions. Microb. Cell Fact. 5 (2006) 1-13
14. Hohenblum H., Naschberger S., Weik R., Katinger H., and Mattanovich D. Production of recombinant human trypsinogen in Escherichia coli and Pichia pastoris. A comparison of expression systems. In: Merten O.-W. (Ed). Recombinant Protein Production with Prokaryotic and Eukaryotic cells: A Comparative View of Host Physiology (2002), Kluwer Academic Publishers., Dordrecht, The Netherlands 339-346
15. Mukund M.A., Bannerjee T., Ghosh I., and Datta S. Effect of mRNA secondary structure in the regulation of gene expression: unfolding of stable loop causes the expression of Taq polymerase in E. coli. Curr. Sci. 76 (1999) 1486-1490
16. Olsthoorn R.C.L., Zoog S., and Duin J. Coevolution of RNA helix stability and Shine-Dalgarno complementarity in a translational start region. Mol. Microbiol. 15 (1995) 333-339
17. Ramesh V., De A., and Nagaraja V. Engineering hyperexpression of bacteriophage Mu C protein by removal of secondary structure at the translation initiation region. Protein Eng. 7 (1994) 1053-1057
18. Zuker M. Mfold web server for nucleic acid folding and hybridization prediction. Nucleic Acids Res. 31 (2003) 3406-3415
19. Patti S., DiSilvestre D., and Brusilow W.S. Regulation of the Escherichia coli uncH gene by mRNA secondary structure and translational coupling. Mol. Microbiol. 6 (1992) 3559-3566
20. Anders J.C. Advances in amino acid analysis. BioPharm Int. (2002) 32-39