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Erickson JR, Wu JJ, Goddard G, Tigyi G, Kawanishi K, Tomei LD, Kiefer MC. EDG-2/Vzg-1 couples to the yeast pheromone response pathway selectively in response to lysophosphatidic acid. of special interest J Biol Chem. 273:1998;1506-1510 Functional production of the putative lysophosphatidic acid receptor in an attempt to determine agonist specifity. LPA-mediated activation of the pheromone response pathway in a sst2D yeast strain is presented.
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Higgins DR, Cregg JM: Introduction to Pichia pastoris. In Pichia Protocols, Edited by Higgins DR, Cregg JM, Totowa, NJ: Humana Press: 1-15. [Walker JM (Series editor): Methods in Molecular Biology, Vol 103.] of special interest. These two reviews provide a general overview and fully exploit the power of the P. pastoris expression system.
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41
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Isolation of the Pichia pastoris glyceraldehyd-3-phosphate dehydrogenase gene and regulation and use of its promoter
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of special interest. Characterization of a constitutive promoter which might be suitable for high level production of foreign proteins. In the meantime, vectors containing this promoter can be obtained from Invitrogen, San Diego.
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of special interest. A comprehensive analysis of two tagged GPCRs produced at high level in P. pastoris. Both receptors have amino-terminal fusions to the α-factor prepropeptide and different processing by the Kex2p was observed. Strategies to improve receptor production are analyzed.
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