Exploring membrane protein dynamics by multicolor single quantum dot imaging using wide field, TIRF, and hyperspectral microscopy

Progress in Biomedical Optics and Imaging - Proceedings of SPIE

Volumn 6448, Issue , 2007, Pages

  Lidke, Diane S ^a   Andrews, Nicholas L ^a   Pfeiffer, Janet R ^a   Jones, Howland D T ^b   Sinclair, Michael B ^b   Haaland, David M ^b   Burns, Alan R ^b   Wilson, Bridget S ^a   Oliver, Janet M ^a   Lidke, Keith A ^b,c  

^a UNIVERSITY OF NEW MEXICO SCHOOL OF MEDICINE   (United States)

^b SANDIA NATIONAL LABORATORIES   (United States)

^c UNIVERSITY OF NEW MEXICO   (United States)

Author keywords

Electron microscopy; Fc RI; Hyperspectral; IgE; Quantum dots; Single particle tracking; TIRF

Indexed keywords

ELECTRON MICROSCOPY; FLUORESCENCE; IMAGE ANALYSIS; PROTEINS; SEMICONDUCTOR QUANTUM DOTS;

CURVE RESOLUTION; SINGLE PARTICLE TRACKING (SPT); TOTAL INTERNAL REFLECTION FLUORESCENCE (TIRF);

BIOLOGICAL MEMBRANES;

EID: 34548269656     PISSN: 16057422     EISSN: None     Source Type: Conference Proceeding    
DOI: 10.1117/12.718464     Document Type: Conference Paper

References (20)

1. D. Marguet, P. F. Lenne, H. Rigneault and H. T. He, "Dynamics in the plasma membrane: how to combine fluidity and order", Embo J, 25, pp.3446-57, 2006.
2. M. Dahan, S. Levi, C. Luccardini, P. Rostaing, B. Riveau and A. Triller, "Diffusion dynamics of glycine receptors revealed by single-quantum dot tracking", Science, 302, pp.442-5, 2003.
3. D. S. Lidke, K. A. Lidke, B. Rieger, T. M. Jovin and D. J. Arndt-Jovin, "Reaching out for signals: filopodia sense EGF and respond by directed retrograde transport of activated receptors", Journal of Cell Biology, 170, pp.619-626, 2005.
4. S. C. Garman, B. A. Wurzburg, S. S. Tarchevskaya, J. P. Kinet and T. S. Jardetzky, "Structure of the Fc fragment of human IgE bound to its high-affinity receptor Fc epsilonRI alpha", Nature, 406, pp.259-66, 2000.
5. F. T. Liu, J. W. Bohn, E. L. Ferry, H. Yamamoto, C. A. Molinaro, L. A. Sherman, N. R. Klinman and D. H. Katz, "Monoclonal dinitrophenyl-specific murine IgE antibody: preparation, isolation, and characterization", J Immunol, 124, pp.2728-37, 1980.
6. M. B. Sinclair, J. A. Timlin, D. M. Haaland and M. Werner-Washburne, "Design, construction, characterization, and application of a hyperspectral microarray scanner", Applied Optics, 43, pp.2079-2088, 2004.
7. D. S. Lidke, K. A. Lidke, B. Rieger, T. M. Jovin and D. J. Arndt-Jovin, "Reaching out for signals: filopodia sense EGF and respond by directed retrograde transport of activated receptors", J Cell Biol, 170, pp.619-26, 2005.
8. D. F. Kucik, E. L. Elson and M. P. Sheetz, "Forward transport of glycoproteins on leading lamellipodia in locomoting cells", Nature, 340, pp.315-7, 1989.
9. D. S. Martin, M. B. Forstner and J. A. Kas, "Apparent subdiffusion inherent to single particle tracking", Biophys J, 83, pp.2109-17, 2002.
10. B. S. Wilson, J. R. Pfeiffer and J. M. Oliver, "Observing FcepsilonRI signaling from the inside of the mast cell membrane", Journal of Cell Biology, 149, pp.1131-1142, 2000.
11. K. A. Lidke, B. Rieger, T. M. Jovin and R. Heintzmann, "Superresolution by localization of quantum dots using blinking statistics", Optics Express, 13, pp.7052-7062, 2005.
12. R. J. Ober, S. Ram and E. S. Ward, "Localization accuracy in single-molecule microscopy", Biophys J, 86, pp. 1185-200, 2004.
13. A. Kusumi, Y. Sako and M. Yamamoto, "Confined lateral diffusion of membrane receptors as studied by single particle tracking (nanovid microscopy). Effects of calcium-induced differentiation in cultured epithelial cells", Biophys J, 65, pp.2021-40, 1993.
14. D. S. Lidke, P. Nagy, R. Heintzmann, D. J. Arndt-Jovin, J. N. Post, H. E. Grecco, E. A. Jares-Erijman and T. M. Jovin, "Quantum dot ligands provide new insights into erbB/HER receptor-mediated signal transduction", Nature Biotechnology, 22, pp. 198-203, 2004.
15. D. Axelrod, N. L. Thompson and T. P. Burghardt, "Total internal inflection fluorescent microscopy", J Microsc, 129, pp.19-28, 1983.
16. D. Loerke, B. Preitz, W. Stuhmer and M. Oheim, "Super-resolution measurements with evanescent-wave fluorescence excitation using variable beam incidence", J Biomed Opt, 5, pp.23-30, 2000.
17. J. L. Thomas, T. J. Feder and W. W. Webb, "Effects of protein concentration on IgE receptor mobility in rat basophilic leukemia cell plasma membranes", Biophys J, 61, pp.1402-12, 1992.
18. M. H. Van Benthem, M. R. Keenan and D. M. Haaland, "Application of equality constraints on variables during alternating least squares procedures", Journal of Chemometrics, 16, pp.613-622, 2002.
19. D. M. Haaland, J. A. Timlin, M. B. Sinclair, M. H. Van Benthem, M. J. Martinez, A. D. Aragon and M. Werner-Washburne, "Multivariate curve resolution for hyperspectral image analysis: applications to microarray technology", In Spectral Imaging: Instrumentation, Applications, and Analysis, 4959, pp.55-66, 2003.
20. J. Zhang, K. Leiderman, J. R. Pfeiffer, B. S. Wilson, J. M. Oliver and S. L. Steinberg, "Characterizing the topography of membrane receptors and signaling molecules from spatial patterns obtained using nanometer-scale electron-dense probes and electron microscopy", Micron, 37, pp. 14-34, 2006.