Production of synthetically created phospholipase A2 variants with industrial impact

Biotechnology and Bioengineering

Volumn 98, Issue 1, 2007, Pages 48-59

  Markert, Yvonne ^a,c   Mansfeld, Johanna ^a   Schierhorn, Angelika ^a   Rücknagel, Karl Peter ^a,b   Ulbrich Hofmann, Renate ^a  

^a MARTIN LUTHER UNIVERSITY HALLE WITTENBERG   (Germany)

^b MAX PLANCK RESEARCH UNIT FOR ENZYMOLOGY OF PROTEIN FOLDING   (Germany)

^c ROCHE DIAGNOSTICS GMBH   (Germany)

Author keywords

Bee venom; Escherichia coli; High cell density fermentation inclusion bodies; Phospholipase A2; Renaturation

Indexed keywords

BIOSYNTHESIS; ESCHERICHIA COLI; GENE EXPRESSION; ION EXCHANGE; OPTIMIZATION; THERMODYNAMIC STABILITY;

BEE VENOM; HIGH CELL DENSITY FERMENTATION INCLUSION BODIES; RENATURATION;

ENZYMES;

BEE VENOM; ENZYME VARIANT; GUANIDINE; LYSOPHOSPHOLIPID; PHOSPHOLIPASE A2; POLYHISTIDINE TAG;

AMINO TERMINAL SEQUENCE; ARTICLE; CELL DENSITY; CELL INCLUSION; CIRCULAR DICHROISM; CONTROLLED STUDY; ENZYME ACTIVITY; ENZYME CONFORMATION; ENZYME ISOLATION; ENZYME PURIFICATION; ENZYME RENATURATION; ENZYME STABILITY; ENZYME SYNTHESIS; ESCHERICHIA COLI; FERMENTATION; GENE EXPRESSION; GENE SYNTHESIS; HONEYBEE; ION EXCHANGE CHROMATOGRAPHY; MOLECULAR WEIGHT; NONHUMAN; SPECTROFLUOROMETRY;

ANIMALS; BEES; ENZYME ACTIVATION; ENZYME STABILITY; ESCHERICHIA COLI; INDUSTRIAL MICROBIOLOGY; PHOSPHOLIPASES A; PROTEIN ENGINEERING; RECOMBINANT PROTEINS; SUBSTRATE SPECIFICITY;

APIS MELLIFERA; APOIDEA; BOVINAE; ESCHERICHIA COLI; MAMMALIA; SUS;

EID: 34548266808     PISSN: 00063592     EISSN: 10970290     Source Type: Journal    
DOI: 10.1002/bit.21392     Document Type: Article

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