Expression of group IA phospholipase A2 in Pichia pastoris: Identification of a phosphatidylcholine activator site using site-directed mutagenesis

Biochemistry

Volumn 38, Issue 43, 1999, Pages 14174-14184

  Lefkowitz, Lee J ^a   Deems, Raymond A ^a   Dennis, Edward A ^a  

^a UNIVERSITY OF CALIFORNIA   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

AMINO ACID; ARGININE; AROMATIC AMINO ACID; ASPARTIC ACID; GLUTAMIC ACID; LYSINE; MUTANT PROTEIN; PHENYLALANINE; PHOSPHATIDYLCHOLINE; PHOSPHATIDYLETHANOLAMINE; PHOSPHOLIPASE A2; SNAKE VENOM; TRYPTOPHAN; TYROSINE;

ARTICLE; CONTROLLED STUDY; ENZYME ACTIVATION; ENZYME ACTIVE SITE; NONHUMAN; PICHIA PASTORIS; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTEIN FOLDING; SITE DIRECTED MUTAGENESIS;

ANIMALS; BINDING SITES; CATALYSIS; CLONING, MOLECULAR; COBRA; ENZYME ACTIVATION; HYDROLYSIS; KINETICS; MICELLES; MODELS, MOLECULAR; MUTAGENESIS, SITE-DIRECTED; PHOSPHATIDYLCHOLINES; PHOSPHOLIPASES A; PICHIA; SURFACE PROPERTIES;

PICHIA PASTORIS; SERPENTES;

EID: 0033607222     PISSN: 00062960     EISSN: None     Source Type: Journal    
DOI: 10.1021/bi991432t     Document Type: Article

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