Development of a screening platform for directed evolution using the reef coral fluorescent protein ZsGreen as a solubility reporter

Protein Engineering, Design and Selection

Volumn 20, Issue 7, 2007, Pages 327-337

  Heddle, Catherine ^a   Mazaleyrat, Sabine L ^b  

^a ASTRAZENECA R AND D   (Sweden)

^b ASTRAZENECA   (United Kingdom)

Author keywords

Directed evolution; E.coli; High throughput solubility screen; Recombinant expression; Reef coral fluorescent proteins (RCFPs)

Indexed keywords

DIRECTED EVOLUTION; RECOMBINANT EXPRESSION; REEF CORAL FLUORESCENT PROTEINS (RCFP);

ESCHERICHIA COLI; FLUORESCENCE; GENE EXPRESSION;

PROTEINS;

EPHRIN RECEPTOR B2; FLUORESCENT PROTEIN ZSGREEN; GREEN FLUORESCENT PROTEIN; HYBRID PROTEIN; UNCLASSIFIED DRUG;

ARTICLE; CORAL REEF; ESCHERICHIA COLI; FLUORESCENCE; GENE MUTATION; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; PROTEIN DOMAIN; PROTEIN ENGINEERING; PROTEIN EXPRESSION;

DIRECTED MOLECULAR EVOLUTION; ESCHERICHIA COLI; GREEN FLUORESCENT PROTEINS; HUMANS; RECEPTOR, EPHB2; RECOMBINANT FUSION PROTEINS; SOLUBILITY;

ANTHOZOA; ESCHERICHIA COLI;

EID: 34548218408     PISSN: 17410126     EISSN: 17410134     Source Type: Journal    
DOI: 10.1093/protein/gzm024     Document Type: Article

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