Assessing GPCR activation using protein complementation: A novel technique for HTS

Biochemical Society Transactions

Volumn 35, Issue 4, 2007, Pages 746-748

  Eglen, R M ^a  

^a PERKINELMER INC   (United States)

Author keywords

Bioluminescence resonance energy transfer (BRET); Enzyme fragment complementation (EFC); Fluorescence resonance energy transfer (FRET); G protein coupled receptor screening (GPCR screening); Protein complementation

Indexed keywords

G PROTEIN COUPLED RECEPTOR;

CATALYSIS; COMPLEMENT FIXATION TEST; CONFERENCE PAPER; LUMINESCENCE; OLIGOMERIZATION; PRIORITY JOURNAL; PROTEIN PROTEIN INTERACTION; SENSITIVITY ANALYSIS; SIGNAL TRANSDUCTION;

EID: 34548156273     PISSN: 03005127     EISSN: None     Source Type: Journal    
DOI: 10.1042/BST0350746     Document Type: Conference Paper

References (18)

1. Eglen, R.M. (2005) Comb. Chem. High Throughput Screen. 8, 311-318
2. Eglen, R.M., Bosse, R. and Reisine, T. (2007) Assay Drug Dev. Technol., in the press
3. Pfleger, K.D.G. and Eidne, K.A. (2005) Biochem. J. 385, 625-637
4. Piehler, J. (2005) Curr. Opin. Struct. Biol. 15, 4-14
5. Bei, M. (2004) Cell. Signalling 16, 175-186
6. Boute, N., Jockers, R. and Issad, T. (2002) Trends Pharmacol. Sci. 23, 351-354
7. Olson, K.R. and Eglen, R.M. (2007) Assay Drug Dev. Technol. 5, 137-144
8. Remy, I. and Michnick, S.W. (1999) Proc. Natl. Acad. Sci. U.S.A. 96, 5394-5399
9. Javitch, J.A. (2004) Mol. Pharmacol. 66, 1077-1082
10. James, J.R., Oliveira, M.I., Carmo, A.M., Iaboni, A. and Davis, S.J. (2006) Nat. Methods 3, 1001-1006
11. Bouvier, M., Heveker, N., Jockers, R., Marullo, S. and Milligan, G. (2007) Nat. Methods 4, 3-4
12. Oakley, R.H., Hudson, C.C., Cruickshank, R.D., Meyers, D.M., Payne, Jr, R.E., Rhem, S.M. and Loomis, C.R. (2002) Assay Drug Dev. Technol. 1, 21-30
13. Heding, A. (2004) Expert Rev. Mol. Diag. 4, 403-411
14. Carter, A.A. and Hill, S.J. (2005) J. Pharmacol. Exp. Ther. 315, 839-848
15. Wehrman, T.S., Casipit, C.L., Gewertz, N.M. and Blau, H.M. (2005) Nat. Methods 2, 521-527
16. Yu, H., West, M., Keon, B.H., Graham, K., Bilter, G.K., Owens, S., Lamerdin, J. and Westwick, J.K. (2003) Assay Drug Dev. Technol. 1, 811-822
17. MacDonald, M.L., Lamerdin, J., Owens, J., Keon, B.H., Bilter, G.K., Strang, Z., Huang, Z., Yu, H., Dias, J., Minami, T. et al. (2006) Nat. Chem. Biol. 2, 329-337
18. Elster, L., Elling, C. and Heding, A. (2007) J. Biomol. Screen. 12, 41-49