Variable pressure and environmental scanning electron microscopy: Imaging of biological samples

Methods in Molecular Biology

Volumn 369, Issue , 2007, Pages 467-495

  Griffin, Brendan J ^a  

^a NONE

Author keywords

Charge contrast imaging; ESEM; Hydrated samples; Uncoated samples; VPSEM

Indexed keywords

ANIMAL; ARTICLE; BLOOD VESSEL; CELL CULTURE; GAS; HYDROSTATIC PRESSURE; INSECT; INSTRUMENTATION; METHODOLOGY; MOUSE; POLLEN; RAT; SCANNING ELECTRON MICROSCOPY; SPIRULINA; ULTRASTRUCTURE; VACUUM;

ANIMALS; BLOOD VESSELS; GASES; HYDROSTATIC PRESSURE; INSECTS; MICE; MICROSCOPY, ELECTRON, SCANNING; POLLEN; RATS; SPIRULINA; TUMOR CELLS, CULTURED; VACUUM;

EID: 34548030662     PISSN: 10643745     EISSN: None     Source Type: Book Series    
DOI: 10.1385/1-59745-294-7:467     Document Type: Article

References (24)

1. Robinson, V. N. E. and Robinson, B. W. (1978) Materials characterisation in a scanning electron microscope environmental cell. Scanning Electron Microsc. 1, 595-602.
2. Danilatos, G. C. (1990) Theory of gaseous detector device in the environmental scanning electron microscope. Adv. Electronics Electron Phys. 71, 1-102.
3. Peters, K. R. (1992) Principles of low vacuum scanning electron microscopy. Proc. 50th Ann. Meeting Electron Microsc. Soc. Am. (Bailey, G.W., Bentley, J., and Small, J.A., ed.), pp. 1304-1305.
4. Danilatos, G. D. and Robinson, V. N. E. (1979) Principles of scanning electron microscopy at high pressures. Scanning 2, 72-82.
5. Farley, A. N. and Shah, J. S. (1988) A new technique for high pressure SEM. Inst. Phys. Conf Ser. 93, 241-242.
6. Knowles, W. R., Thiel, B. L., Toth, M., et al. (2004) Design of a two-stage gas amplification secondary electron detector for imaging insulating samples at the sub-1 nm scale. Microsc. Microanal. 10, 1060-1061.
7. Thiel, B. T., Toth, M., and Knowles, W. R. (2005) Ultra-high resolution and metrology with Low Vacuum SEM. Microsc. Microanal. 11, 384CD.
8. Fletcher A., Thiel, B. L., and Donald, A. M. (1997) Amplification measurements of alternative imaging gases in ESEM. J. Phys. D: Appl. Phys. 30, 2249-2257.
9. Thiel, B. L., Bache, I. C., Fletcher, A. L., Meredith, P., and Donald, A. M. (1997) An improved model for gaseous amplification in the environmental SEM. J. Microsc. (Oxford) 187, 143-157.
10. Phillips, M. R. and Morgan, S. W. (2005) Direct comparison of various gaseous secondary electron detectors in the variable pressure scanning electron microscope. Microsc. Microanal. 11, 398-399.
11. Thiel, B. L. and Toth, M. (2005) Secondary electron contrasts in low-vacuum/ environmental scanning electron microscopy of dielectrics. J. Appl. Physics 97, 1-18.
12. Craven, J. P., Baker, F. S., Thiel, B. L., and Donald, A. M. (2002) Consequences of positive ions upon imaging in low vacuum scanning electron microscopy. J. Microsc. (Oxford) 205, 96-105.
13. Stokes, D. J., Baker, F. S., and Toth, M. (2004) Raising the pressure: realizing room temperature/high humidity applications in ESEM. Microsc. Microanal, 10, 1074-1075.
14. Griffin, B. J. and Browne, J. R. (2000) Fibre-optic based spectral cathodoluminescence: simple and economic option for use in convectional and environmental scanning electron microscopy. Microsc. Microanal. 6, 42-48.
15. Griffin, B. J., van Riessen, A., and Egerton-Warburton, L. (1995) A review of detection strategies and imaging of hydrated biological specimens in the environmental SEM. Scanning 17, 58-59.
16. Griffin, B. J. (2000) Charge contrast imaging of material growth and defects in Environmental Scanning Electron Microscopy-linking electron emission and cathodoluminescence. Scanning 22, 234-242.
17. Roach, G. I. D., Cornell, J. B., and Griffin, B. J. ( 1998) Gibbsite growth historyrevelations of a new scanning electron microscope technique, in Light Metals 1998 (Welch, B., ed.), TMS Publications, pp. 153-158.
18. Stokes, D. J., Thiel, B. L., and Donald, A. M. (1998) Direct observation of wateroil emulsion systems in the liquid state by environmental SEM. Langmuir 14, 4402-4408.
19. Clode, P. L. and Griffin, B. J. (2004) Charge contrast imaging of the soft tissue and mineralized skeletal phases in a scleractinian coral. Microsc. Microanal. 10, 1078-1079.
20. McMenamin, P. G., Djano, J., Wealthall, R., and Griffin, B. J. (2002) Characterization of the macrophages associated with the tunica vasculosa lentis of the rat eye. Invest. Ophthalmol. Visual Sci. 43, 2076-2082.
21. Joy, D. C. (1995) Monte Carlo Modeling for Microscopy and Microanalysis. Oxford University Press, New York.
22. Griffin, B. J. (2005) Challenges and progress towards low voltage imaging in VPSEM. Microsc. Microanal. 11, 396-397.
23. Stokes, D. J. (2003) Recent advances in electron imaging, image interpretation and applications: environmental scanning electron microscopy. Phil. Trans. R. Soc. Loud. A., 361, 2771-2787.
24. Stokes, D. J., Baker, F. S., and Toth, M. (2004) Raising the pressure: realizing room temperature/high humidity applications in ESEM. Microsc. Microanal. 10, 1074-1075.