Proteomic Investigation of Human Burn Wounds by 2D-Difference Gel Electrophoresis and Mass Spectrometry

Journal of Surgical Research

Volumn 142, Issue 1, 2007, Pages 143-152

  Pollins, Alonda C ^a   Friedman, David B ^a   Nanney, Lillian B ^a  

^a VANDERBILT UNIVERSITY SCHOOL OF MEDICINE   (United States)

Author keywords

2D DIGE; burns; proteomics; skin; wound healing

Indexed keywords

BIOLOGICAL MARKER; CELL CYCLE PROTEIN 37; CYTOKERATIN 1; CYTOKERATIN 16; CYTOKERATIN 17; CYTOKERATIN 5; CYTOKERATIN 6; CYTOSKELETON PROTEIN; GELSOLIN; HAPTOGLOBIN; INITIATION FACTOR 4A; IQ MOTIF CONTAINING GUANOSINE TRIPHOSPHATASE ACTIVATING PROTEIN 1; SERINE PROTEINASE INHIBITOR; TISSUE EXTRACT; TUMOR PROTEIN; VIMENTIN;

ADOLESCENT; ADULT; ARTICLE; BURN; CLINICAL ARTICLE; CONTROLLED STUDY; FEMALE; HUMAN; HUMAN TISSUE; MALE; MATRIX ASSISTED LASER DESORPTION IONIZATION TIME OF FLIGHT MASS SPECTROMETRY; PRESCHOOL CHILD; PRIORITY JOURNAL; PROTEIN DETERMINATION; PROTEIN EXPRESSION; PROTEOMICS; SCHOOL CHILD; TWO DIMENSIONAL GEL ELECTROPHORESIS; WOUND HEALING;

ADOLESCENT; ADULT; BURNS; CHILD; CYTOSKELETAL PROTEINS; ELECTROPHORESIS, GEL, TWO-DIMENSIONAL; FEMALE; GENE EXPRESSION PROFILING; HEAT-SHOCK PROTEINS; HUMANS; INFLAMMATION; MALE; MASS SPECTROMETRY; MIDDLE AGED; MULTIVARIATE ANALYSIS; PROTEIN ISOFORMS; PROTEOMICS; WOUND HEALING;

EID: 34547960733     PISSN: 00224804     EISSN: 10958673     Source Type: Journal    
DOI: 10.1016/j.jss.2007.01.001     Document Type: Article

References (50)

1. Gibran N.S., and Heimbach D.M. Current status of burn wound pathophysiology. Clin Plast Surg 27 (2000) 11
2. Nanney L.B., Wenczak B.A., and Lynch J.B. Progressive burn injury documented with vimentin immunostaining. J Burn Care Rehabil 17 (1996) 191
3. Herndon D.N. Total Burn Care (2002), W.B. Saunders, London
4. Harrison C.A., Gossiel F., Bullock A.J., et al. Investigation of keratinocyte regulation of collagen I synthesis by dermal fibroblasts in a simple in vitro model. Br J Dermatol 154 (2006) 401
5. Lofts J.A. Cost analysis of a major burn. NZ Med J 104 (1991) 488
6. Akita S., Akino K., Imaizumi T., et al. A basic fibroblast growth factor improved the quality of skin grafting in burn patients. Burns 31 (2005) 855
7. Mann R., and Heimbach D. Prognosis and treatment of burns. West J Med 165 (1996) 215
8. Brigham P.A., and McLoughlin E. Burn incidence and medical care use in the United States: Estimates, trends, and data sources. J Burn Care Rehabil 17 (1996) 95
9. Huang C., Foster K.W., DeSilva T., et al. Comparative proteomic profiling of murine skin. J Invest Dermatol 121 (2003) 51
10. Gromov P., Skovgaard G.L., Palsdottir H., et al. Protein profiling of the human epidermis from elderly reveals up-regulation of a signature of interferon-gamma-induced polypeptides that includes manganese-superoxide dismutase and the p85beta subunit of phosphatidylinositol 3-kinase. Mol Cell Proteomics 2 (2003) 70
11. Ibuki Y., Naitou H., Ohashi N., et al. Proteome analysis of UV-B-induced anti-apoptotic regulatory factors. Photochem Photobiol 81 (2005) 823
12. Alban A., David S.O., Bjorkesten L., et al. A novel experimental design for comparative two-dimensional gel analysis: Two-dimensional difference gel electrophoresis incorporating a pooled internal standard. Proteomics 3 (2003) 36
13. Lilley K.S., and Friedman D.B. All about DIGE: Quantification technology for differential-display 2D-gel proteomics. Exp Rev Proteomics 1 (2004) 401
14. Friedman D.B., Wang S.E., Whitwell C.W., et al. Multi-variable difference gel electrophoresis and mass spectrometry: A case study on TGF-beta and ErbB2 signaling. Mol Cell Proteomics 1 (2007) 150
15. Laemmli U.K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227 (1970) 680
16. Ogura H., Hashiguchi N., Tanaka H., et al. Long-term enhanced expression of heat shock proteins and decelerated apoptosis in polymorphonuclear leukocytes from major burn patients. J Burn Care Rehabil 23 (2002) 103
17. Diao L., Li C., and Sheng Z. [The significance of the expression of the HSP70 and HSP90 in the intestinal mucosa in scalded rats during early postburn stage]. Zhonghua Shao Shang Za Zhi 16 (2000) 279
18. Kabasakal L., Sener G., Cetinel S., et al. Burn-induced oxidative injury of the gut is ameliorated by the leukotriene receptor blocker montelukast. Prostaglandins Leukot Essent Fatty Acids 72 (2005) 431
19. Kubo M., Van de Water L., Plantefaber L.C., et al. Fibrinogen and fibrin are anti-adhesive for keratinocytes: A mechanism for fibrin eschar slough during wound repair. J Invest Dermatol 117 (2001) 1369
20. Mosesson M.W., Siebenlist K.R., and Meh D.A. The structure and biological features of fibrinogen and fibrin. Ann NY Acad Sci 936 (2001) 11
21. de Kleijn D.P., Smeets M.B., Kemmeren P.P., et al. Acute-phase protein haptoglobin is a cell migration factor involved in arterial restructuring. FASEB J 16 (2002) 1123
22. Grinnell F., and Zhu M. Identification of neutrophil elastase as the proteinase in burn wound fluid responsible for degradation of fibronectin. J Invest Dermatol 103 (1994) 155
23. Faymonville M.E., Micheels J., Bodson L., et al. Biochemical investigations after burning injury: Complement system, protease-antiprotease balance and acute-phase reactants. Burns Incl Therm Inj 13 (1987) 26
24. Doumas S., Kolokotronis A., and Stefanopoulos P. Anti-inflammatory and antimicrobial roles of secretory leukocyte protease inhibitor. Infect Immun 73 (2005) 1271
25. Zhu J., Nathan C., Jin W., et al. Conversion of proepithelin to epithelins: Roles of SLPI and elastase in host defense and wound repair. Cell 111 (2002) 867
26. Thuraisingam T., Sam H., Moisan J., et al. Delayed cutaneous wound healing in mice lacking solute carrier 11a1 (formerly Nramp1): Correlation with decreased expression of secretory leukocyte protease inhibitor. J Invest Dermatol 126 (2006) 890
27. Odaka C., Mizuochi T., Yang J., et al. Murine macrophages produce secretory leukocyte protease inhibitor during clearance of apoptotic cells: implications for resolution of the inflammatory response. J Immunol 171 (2003) 1507
28. Sorensen O.E., Cowland J.B., Theilgaard-Monch K., et al. Wound healing and expression of antimicrobial peptides/polypeptides in human keratinocytes, a consequence of common growth factors. J Immunol 170 (2003) 5583
29. Lai J.Y., Borson N.D., Strausbauch M.A., et al. Mitosis increases levels of secretory leukocyte protease inhibitor in keratinocytes. Biochem Biophys Res Commun 316 (2004) 407
30. Wingens M., van Bergen B.H., Hiemstra P.S., et al. Induction of SLPI (ALP/HUSI-I) in epidermal keratinocytes. J Invest Dermatol 111 (1998) 996
31. Ashcroft G.S., Lei K., Jin W., et al. Secretory leukocyte protease inhibitor mediates non-redundant functions necessary for normal wound healing. Nat Med 6 (2000) 1147
32. Cataltepe S., Gornstein E.R., Schick C., et al. Co-expression of the squamous cell carcinoma antigens 1 and 2 in normal adult human tissues and squamous cell carcinomas. J Histochem Cytochem 48 (2000) 113
33. Schick C., Kamachi Y., Bartuski A.J., et al. Squamous cell carcinoma antigen 2 is a novel serpin that inhibits the chymotrypsin-like proteinases cathepsin G and mast cell chymase. J Biol Chem 272 (1997) 1849
34. Mitsuishi K., Nakamura T., Sakata Y., et al. The squamous cell carcinoma antigens as relevant biomarkers of atopic dermatitis. Clin Exp Allergy 35 (2005) 1327
35. Katz A.B., and Taichman L.B. A partial catalog of proteins secreted by epidermal keratinocytes in culture. J Invest Dermatol 112 (1999) 818
36. Katagiri C., Nakanishi J., Kadoya K., et al. Serpin squamous cell carcinoma antigen inhibits UV-induced apoptosis via suppression of c-JUN NH2-terminal kinase. J Cell Biol 172 (2006) 983
37. Akiyama M., Inamoto N., and Nakamura K. Malignant melanoma and squamous cell carcinoma forming one tumour on a burn scar. Dermatology 194 (1997) 157
38. Lee S.H., Shin M.S., Kim H.S., et al. Somatic mutations of Fas (Apo-1/CD95) gene in cutaneous squamous cell carcinoma arising from a burn scar. J Invest Dermatol 114 (2000) 122
39. Bommer U.A., and Thiele B.J. The translationally controlled tumour protein (TCTP). Int J Biochem Cell Biol 36 (2004) 379
40. Wells A., Ware M.F., Allen F.D., et al. Shaping up for shipping out: PLCgamma signaling of morphology changes in EGF-stimulated fibroblast migration. Cell Motil Cytoskeleton 44 (1999) 227
41. Xie H., Pallero M.A., Gupta K., et al. EGF receptor regulation of cell motility: EGF induces disassembly of focal adhesions independently of the motility-associated PLCgamma signaling pathway. J Cell Sci 111 Pt. 5 (1998) 615
42. Witke W., Sharpe A.H., Hartwig J.H., et al. Hemostatic, inflammatory, and fibroblast responses are blunted in mice lacking gelsolin. Cell 81 (1995) 41
43. Cavalieri S., Rotoli M., Feliciani C., et al. Expression of the high-affinity laminin receptor (67 kDa) in normal human skin and appendages. Int J Immunopathol Pharmacol 18 (2005) 223
44. Zhou L., Xie M., Zhou J.Q., et al. 67-kDa laminin receptor in human laryngeal squamous cell carcinoma. Laryngoscope 116 (2006) 28
45. Sugimoto N., Imoto I., Fukuda Y., et al. IQGAP1, a negative regulator of cell-cell adhesion, is upregulated by gene amplification at 15q26 in gastric cancer cell lines HSC39 and 40A. J Hum Genet 46 (2001) 21
46. Watanabe T., Wang S., Noritake J., et al. Interaction with IQGAP1 links APC to Rac1, Cdc42, and actin filaments during cell polarization and migration. Dev Cell 7 (2004) 871
47. Kubler M.D., Jordan P.W., O'Neill C.H., et al. Change in the abundance and distribution of actin and associated proteins during terminal differentiation of human epidermal keratinocytes. J Cell Sci 100 (1991) 153
48. Bernot K.M., Coulombe P.A., and McGowan K.M. Keratin 16 expression defines a subset of epithelial cells during skin morphogenesis and the hair cycle. J Invest Dermatol 119 (2002) 1137
49. Mazzalupo S., Wong P., Martin P., et al. Role for keratins 6 and 17 during wound closure in embryonic mouse skin. Dev Dyn 226 (2003) 356
50. Ikegawa S., Saida T., Takizawa Y., et al. Vimentin-positive squamous cell carcinoma arising in a burn scar. A highly malignant neoplasm composed of acantholytic round keratinocytes. Arch Dermatol 125 (1989) 1672