Involvement of Gly 311 residue on substrate discrimination, pH and temperature dependency of recombinant Staphylococcus xylosus lipase: A study with emulsified substrate

Protein Expression and Purification

Volumn 55, Issue 1, 2007, Pages 31-39

  Mosbah, Habib ^a   Sayari, Adel ^a   Horchani, Habib ^a   Gargouri, Youssef ^a  

^a UNIVERSITY OF SFAX   (Tunisia)

Author keywords

Catalytic efficiency; Expression; Phospholipase activity; Purification; Site directed mutagenesis; Staphylococcus xylosus lipase; Steric hindrance; Substrate discrimination

Indexed keywords

BACTERIAL PROTEIN; GLYCINE; PHOSPHOLIPASE; RECOMBINANT PROTEIN; TRIACYLGLYCEROL LIPASE;

ARTICLE; BIOSYNTHESIS; CHEMISTRY; ENZYME SPECIFICITY; ENZYMOLOGY; ESCHERICHIA COLI; GENETICS; KINETICS; MUTATION; PH; PLASMID; SITE DIRECTED MUTAGENESIS; STAPHYLOCOCCUS; TEMPERATURE;

BACTERIAL PROTEINS; ESCHERICHIA COLI; GLYCINE; HYDROGEN-ION CONCENTRATION; KINETICS; LIPASE; MUTAGENESIS, SITE-DIRECTED; MUTATION; PHOSPHOLIPASES; PLASMIDS; RECOMBINANT PROTEINS; STAPHYLOCOCCUS; SUBSTRATE SPECIFICITY; TEMPERATURE;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI; STAPHYLOCOCCUS SIMULANS; STAPHYLOCOCCUS XYLOSUS;

EID: 34547913055     PISSN: 10465928     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.pep.2007.04.008     Document Type: Article

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