Importance of the residue Asp 290 on chain length selectivity and catalytic efficiency of recombinant Staphylococcus simulans lipase expressed in E. coli

Molecular Biotechnology

Volumn 36, Issue 1, 2007, Pages 14-22

  Sayari, Adel ^a   Mosbah, Habib ^a   Gargouri, Youssef ^a  

^a UNIVERSITY OF SFAX   (Tunisia)

Author keywords

Asp290Ala mutant lipase; Expression; Kinetic parameters; Purification; Site direct mutagenesis; Staphylococcus simulans lipase; Substrate selectivity

Indexed keywords

BACTERIA; CATALYSIS; CATALYSTS; POSITRON EMISSION TOMOGRAPHY; PROTEINS;

ASP290ALA MUTANT LIPASE; KWD SITE DIRECT MUTAGENESIS; STAPHYLOCOCCUS SIMULANS LIPASE; SUBSTRATE SELECTIVITY;

CHAIN LENGTH;

ASPARTIC ACID; NICKEL; TRIACYLGLYCEROL LIPASE; CALCIUM; DETERGENT; MUTANT PROTEIN; RECOMBINANT PROTEIN; TRIACYLGLYCEROL; TRIBUTYRIN; TRIOLEIN;

AMINO TERMINAL SEQUENCE; ARTICLE; CATALYSIS; CONTROLLED STUDY; ESCHERICHIA COLI; HYDROLYSIS; KINETICS; NONHUMAN; PROTEIN EXPRESSION; PROTEIN PURIFICATION; STAPHYLOCOCCUS; STAPHYLOCOCCUS HYICUS; WILD TYPE; BACTERIAL GENE; DRUG EFFECT; EMULSION; ENZYME SPECIFICITY; ENZYME STABILITY; ENZYMOLOGY; GENE EXPRESSION; GENETICS; ISOLATION AND PURIFICATION; METABOLISM; MOLECULAR CLONING; MUTATION; PH; PLASMID; STRUCTURE ACTIVITY RELATION; TEMPERATURE;

STAPHYLOCOCCUS HYICUS; STAPHYLOCOCCUS SIMULANS;

ASPARTIC ACID; CALCIUM; CATALYSIS; CLONING, MOLECULAR; DETERGENTS; EMULSIONS; ENZYME STABILITY; ESCHERICHIA COLI; GENE EXPRESSION; GENES, BACTERIAL; HYDROGEN-ION CONCENTRATION; KINETICS; LIPASE; MUTANT PROTEINS; MUTATION; PLASMIDS; RECOMBINANT PROTEINS; STAPHYLOCOCCUS; STRUCTURE-ACTIVITY RELATIONSHIP; SUBSTRATE SPECIFICITY; TEMPERATURE; TRIGLYCERIDES; TRIOLEIN;

EID: 34447252433     PISSN: 10736085     EISSN: None     Source Type: Journal    
DOI: 10.1007/s12033-007-0008-2     Document Type: Article

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