Expression, purification, and characterization of His-tagged Staphylococcus xylosus lipase wild-type and its mutant Asp 290 Ala

Protein Expression and Purification

Volumn 47, Issue 2, 2006, Pages 516-523

  Mosbah, Habib ^a   Sayari, Adel ^a   Bezzine, Sofiane ^a   Gargouri, Youssef ^a  

^a UNIVERSITY OF SFAX   (Tunisia)

Author keywords

Asp290 mutant lipase; Overexpression; Purification; Site directed mutagenesis; Staphylococcus xylosus lipase

Indexed keywords

BACTERIAL PROTEIN; HYBRID PROTEIN; TRIACYLGLYCEROL LIPASE;

AFFINITY CHROMATOGRAPHY; AMINO ACID SUBSTITUTION; ARTICLE; BIOSYNTHESIS; CATALYSIS; CHEMISTRY; ENZYME SPECIFICITY; ENZYMOLOGY; ESCHERICHIA COLI; GENETICS; ISOLATION AND PURIFICATION; POINT MUTATION; SITE DIRECTED MUTAGENESIS; STAPHYLOCOCCUS;

AMINO ACID SUBSTITUTION; BACTERIAL PROTEINS; CATALYSIS; CHROMATOGRAPHY, AFFINITY; ESCHERICHIA COLI; LIPASE; MUTAGENESIS, SITE-DIRECTED; POINT MUTATION; RECOMBINANT FUSION PROTEINS; STAPHYLOCOCCUS; SUBSTRATE SPECIFICITY;

ESCHERICHIA COLI; STAPHYLOCOCCUS XYLOSUS;

EID: 33646591284     PISSN: 10465928     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.pep.2005.11.013     Document Type: Article

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