Single-tube nested PCR using immobilized internal primers for the identification of dengue virus serotypes

Journal of Virological Methods

Volumn 145, Issue 1, 2007, Pages 76-79

  Gomes, A L V ^a   Silva, A M ^a   Cordeiro, M T ^a   Guimaraes G F ^a   Marques Jr , E T A ^a,b   Abath, F G C ^a  

^a INSTITUTO OSWALDO CRUZ   (Brazil)

^b JOHNS HOPKINS UNIVERSITY SCHOOL OF MEDICINE   (United States)

Author keywords

Dengue; Diagnosis; Nested PCR; Single tube

Indexed keywords

ARTICLE; CONFIDENCE INTERVAL; CONTROLLED STUDY; COST BENEFIT ANALYSIS; DENGUE VIRUS; DENGUE VIRUS 1; DENGUE VIRUS 2; DENGUE VIRUS 3; DIAGNOSTIC VALUE; INTERMETHOD COMPARISON; NONHUMAN; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; SENSITIVITY AND SPECIFICITY; SEROTYPE; SINGLE TUBE NESTED POLYMERASE CHAIN REACTION; TIME; VIRUS DIAGNOSIS; VIRUS IDENTIFICATION; VIRUS TYPING;

DENGUE; DENGUE VIRUS; DNA PRIMERS; DNA, COMPLEMENTARY; HUMANS; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; RNA, VIRAL; SENSITIVITY AND SPECIFICITY; SEROTYPING;

DENGUE VIRUS; DENGUE VIRUS GROUP;

EID: 34547880745     PISSN: 01660934     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jviromet.2007.05.003     Document Type: Article

References (14)

1. Abath F.G., Melo F.L., Werkhauser R.P., Montenegro L., Montenegro R., and Schindler H.C. Single-tube nested PCR using immobilized internal primers. Biotechniques 33 (2002) 1210-1214
2. Anwar A., August J.T., and Too H.P. A stem-loop-mediated reverse transcription real-time PCR for the selective detection and quantification of the replicative strand of an RNA virus. Anal. Biochem. 352 (2006) 120-128
3. Chakravarti A., and Kumaria R. Molecular detection and serotypic characterization of dengue viruses by single-tube multiplex reverse transcriptase-polymerase chain reaction. Diagn. Microbiol. Infect. Dis. 52 (2005) 311-316
4. Chien L.J., Liao T.L., Shu P.Y., Huang J.H., Gubler D.J., and Chang G.J.J. Development of real-time reverse transcriptase PCR assays to detect and serotype dengue viruses. J. Clin. Microbiol. 44 (2006) 1295-1304
5. Guha-Sapir D., and Schimmer B. Dengue fever: new paradigms for a changing epidemiology. Emerg. Themes Epidemiol. 2 (2005) 1
6. Guzman M.G., and Kouri G. Dengue diagnosis, advances and challenges. Int. J. Infect. Dis. 8 (2004) 69-80
7. Harris E., Roberts T.G., Smith L., Selle J., Kramer L.D., Valle S., Sandoval E., and Balmaseda A. Typing of dengue viruses in clinical specimens and mosquitoes by single-tube multiplex reverse transcriptase PCR. J. Clin. Microbiol. 36 (1998) 2634-2639
8. Harris E., Videa E., Perez L., Sandoval E., Tellez Y., Perez M.A., Cuadra R., Rocha J., Idiaquez W., Alonso R.E., Delgado M.A., Campo L.A., Acevedo F., Gonzalez A., Amador J.J., and Balmaseda A. Clinical, epidemiologic, and virologic features of dengue in the 1998 epidemic in Nicaragua. Am. J. Trop. Med. Hyg. 63 (2000) 5-11
9. Kong Y.Y., Thay C.H., Tin T.C., and Devi S. Rapid detection, serotyping and quantitation of dengue viruses by TaqMan real-time one-step RT-PCR. J. Virol. Methods 138 (2006) 123-130
10. Lanciotti R.S., Calisher C.H., Gubler D.J., Chang G.J., and Vorndam A.V. Rapid detection and typing of dengue viruses from clinical samples by using reverse transcriptase-polymerase chain reaction. J. Clin. Microbiol. 30 (1992) 545-551
11. Melo F.L., Gomes A.L.d.V., Barbosa C.S., Werkhauser R.P., and Abath F.G.C. Development of molecular approaches for the identification of transmission sites of schistosomiasis. Trans. R. Soc. Trop. Med. Hyg. 100 (2006) 1049-1055
12. Montenegro L.M.L., Montenegro R.A., Lima A.S., Carvalho A.B., Schindler H.C., and Abath F.G.C. Development of a single-tube hemi-nested PCR for genus specific detection of Plasmodium in oligoparasitemic patients. Trans. R. Soc. Trop. Med. Hyg. 98 (2004) 616-625
13. Picken M.M., Picken R.N., Han D., Cheng Y., and Strle F. Single-tube nested polymerase chain reaction assay based on flagellin gene sequences for detection of Borrelia burgdorferi sensu lato. Eur. J. Clin. Microbiol. Infec. Dis. 15 (1996) 489-498
14. Rothman A.L. Dengue: defining protective versus pathologic immunity. J. Clin. Invest. 113 (2004) 946-951