A stem-loop-mediated reverse transcription real-time PCR for the selective detection and quantification of the replicative strand of an RNA virus

Analytical Biochemistry

Volumn 352, Issue 1, 2006, Pages 120-128

  Anwar, Azlinda ^a   August, J Thomas ^a,b   Too, Heng Phon ^c  

^a Johns Hopkins Singapore   (Singapore)

^b JOHNS HOPKINS UNIVERSITY SCHOOL OF MEDICINE   (United States)

^c NATIONAL UNIVERSITY OF SINGAPORE   (Singapore)

Author keywords

Real time PCR; Stem loop RT PCR; Virus replication

Indexed keywords

RNA; STRUCTURAL OPTIMIZATION; VIRUSES;

DETECTION SENSITIVITY; REAL-TIME PCR; REAL-TIME PCR METHOD; RESPIRATORY SYNCYTIAL VIRUS; REVERSE TRANSCRIPTION; STEM-LOOP; STEM-LOOP STRUCTURES; VIRUS REPLICATION;

POLYMERASE CHAIN REACTION;

VIRUS RNA;

ARTICLE; GENE SEQUENCE; NONHUMAN; PRIORITY JOURNAL; QUANTITATIVE ANALYSIS; REAL TIME POLYMERASE CHAIN REACTION; RELIABILITY; RESPIRATORY SYNCYTIAL PNEUMOVIRUS; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; RNA VIRUS; VIRUS DETECTION; VIRUS REPLICATION; WEST NILE FLAVIVIRUS;

DENGUE VIRUS; HUMAN RESPIRATORY SYNCYTIAL VIRUS; RESPIRATORY SYNCYTIAL VIRUS; RNA VIRUSES; WEST NILE VIRUS;

EID: 33646020098     PISSN: 00032697     EISSN: 10960309     Source Type: Journal    
DOI: 10.1016/j.ab.2006.01.046     Document Type: Article

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