Determination of lipoxygenase activity in plant extracts using a modified ferrous oxidation-xylenol orange assay

Journal of Agricultural and Food Chemistry

Volumn 55, Issue 15, 2007, Pages 5956-5959

  Pinto, Maria Del Carmen ^a   Tejeda, Adrián ^a   Duque, Antonio L ^a   Macías, Pedro ^a  

^a UNIVERSITY OF EXTREMADURA   (Spain)

Author keywords

Enzymatic activity determination; Lipoxygenase; Xylenol orange

Indexed keywords

FERROUS ION; LIPID PEROXIDE; LIPOXYGENASE; PLANT EXTRACT; UNCLASSIFIED DRUG; XYLENE; XYLENOL ORANGE;

ARTICLE; CHEMISTRY; COLORIMETRY; ENZYMOLOGY; METABOLISM; OXIDATION REDUCTION REACTION; PLANT;

COLORIMETRY; FERROUS COMPOUNDS; LIPID PEROXIDES; LIPOXYGENASE; OXIDATION-REDUCTION; PLANT EXTRACTS; PLANTS; XYLENES;

EID: 34547814756     PISSN: 00218561     EISSN: None     Source Type: Journal    
DOI: 10.1021/jf070537x     Document Type: Article

References (10)

1. Feussner, I.; Wasternack, C. The lipoxygenase pathway. Annu. Rev. Plant Biol. 2002, 53, 275-297.
2. Kühn, H.; Wiesner, R.; Rathman, J.; Schewe, T. Formation of ketodienoic fatty acid by the pure pea lipoxygenase-1. Eicosanoids 1991, 4, 9-14.
3. Axelrod, B.; Chesebrough, T. M.; Laakso, S. Lipoxygenase from soybeans. Methods Enzymol. 1981, 71, 441-445.
4. Khun, H.; Gotze, R.; Schewe, T.; Rapoport, S. M. Quasi-lipoxygenase activity of hemoglobin. A model for lipoxygenase. Eur. J. Biochem. 1981, 120, 161-168.
5. Waslidge, N. B.; Hayes, D. J. A colorimetric method for the determination of lipoxygenase activity suitable for use in high throughput assay format. Anal. Biochem. 1995, 231, 354-358.
6. DeLong, J. M.; Prange, R. K.; Hodges, D. M.; Forney, C. F.; Bishop, M. C.; Quilliam, M. Using a modified ferrous oxidation-xylenol orange (FOX) assay for detection of lipid hydroperoxides in plant tissue. J. Agric. Food Chem. 2002, 50, 248-254.
7. Cho, Y. S.; Kim, H. S.; Kim, C. H.; Cheon, H. G. Application of the ferrous oxidation-xylenol orange assay for the screening of 5-lipoxygenase inhibitors. Anal. Biochem. 2006, 351, 62-68.
8. Gay, C. A.; Gebicki, J. M. Perchloric acid enhances sensitivity and reproducibility of the ferric-xylenol orange peroxide assay. Anal. Biochem. 2002, 304, 42-46.
9. Gay, C. A.; Gebicki, J. M. Measurement of protein and lipid hydroperoxides in biological systems by the ferric-xylenol orange method. Anal. Biochem. 2003, 315, 29-35.
10. Hamberg, M.; Samuelsson, B. On the specificity of the oxygenation of unsaturated fatty acids catalyzed by soybean lipoxydase. J. Biol. Chem. 1967, 242, 5329-5335.