Application of the ferrous oxidation-xylenol orange assay for the screening of 5-lipoxygenase inhibitors

Analytical Biochemistry

Volumn 351, Issue 1, 2006, Pages 62-68

  Cho, Young Sik ^a   Kim, Hyo Sun ^a   Kim, Chi Hyun ^a   Cheon, Hyae Gyeong ^a  

^a Korea Research Institute of Chemical Technology   (South Korea)

Author keywords

5 Lipoxygenase; FOX assay; High throughput screening; Zileuton

Indexed keywords

CALCIUM COMPOUNDS; CITRUS FRUITS; REACTION INTERMEDIATES; REAGENTS;

5-LIPOXYGENASE; 5-LIPOXYGENASE (5-LO); HIGH THROUGHPUT SCREENING; HIGH-THROUGHPUT ASSAYS; INFLAMMATORY DISEASE; OPTIMUM CONCENTRATION; SPECTROPHOTOMETRIC ASSAYS; ZILEUTON;

DIAGNOSIS;

ADENOSINE TRIPHOSPHATE; ARACHIDONATE 5 LIPOXYGENASE; CALCIUM ION; EDETIC ACID; FERROUS ION; HYDROPEROXIDE; LIPOXYGENASE INHIBITOR; PHOSPHATIDYLCHOLINE; REAGENT; UNCLASSIFIED DRUG; XYLENOL ORANGE; ZILEUTON;

ANALYTIC METHOD; ANIMAL CELL; ARTICLE; ASSAY; COMPLEX FORMATION; CONCENTRATION RESPONSE; CONTROLLED STUDY; DRUG EFFECT; ENZYME ACTIVITY; ENZYME INHIBITION; GENE OVEREXPRESSION; HIGH THROUGHPUT SCREENING; IC 50; INSECT CELL; INTERMETHOD COMPARISON; LEPIDOPTERA; NONHUMAN; OXIDATION; PRIORITY JOURNAL; SPECTROPHOTOMETRY;

ANIMALIA; INSECTA; LEPIDOPTERA;

EID: 33644936900     PISSN: 00032697     EISSN: 10960309     Source Type: Journal    
DOI: 10.1016/j.ab.2005.12.025     Document Type: Article

References (16)

1. Y.-Y. Zhang, J.L. Walker, A. Huang, J.F. Keaney Jr., C.B. Clish, C.N. Serhan, and J. Loscalzo Expression of 5-lipoxygenase in pulmonary artery endothelial cells Biochem. J. 361 2002 267 276
2. S.D. Brain, and T.J. Williams Leukotrienes and inflammation Pharmacol. Ther. 46 1990 57 66
3. R. Natarajan, and J.L. Nadler Lipid inflammatory mediators in diabetic vascular disease Arteriosclerosis Thrombosis Vascular Biol. 24 2004 1542 1548
4. W.P. Beierschmitt, J.D. McNeish, R.J. Griffiths, A. Nagahisa, M. Nakane, and D.E. Amacher Induction of hepatic microsomal drug-metabolizing enzymes by inhibitors of 5-lipoxygenase (5-LO): studies in rats and 5-LO knockout mice Toxicol. Sci. 63 2001 15 21
5. J. Breton, P. Keller, M. Chabot-Fletcher, L. Hillegass, W. DeWolf Jr., and D. Griswold Use of a continuous assay of oxygen consumption to evaluate the pharmacology of 5-lipoxygenase inhibitors Prostaglandins Leukotrienes Essent. Fatty Acids 49 1993 929 937
6. N.B Waslidge, and D.J. Hayes A colorimetric method for the determination of lipoxygenase activity suitable for use in a high throughput assay format Anal. Biochem. 231 1995 354 358
7. A.W. Ford-Hutchinson, M. Gresser, and R.N. Young 5-Lipoxygenase Annu. Rev. Biochem. 63 1994 383 417
8. U.K. Laemmli Cleavage of structural proteins during the assembly of the head of bacteriophage T4 Nature 227 1970 680 685
9. M.J. Gibian, and P. Vanderberg Product yield in oxygenation of linoleate by soybean lipoxygenase: the value of the molar extinction coefficient in the spectrophotometric assay Anal. Biochem. 163 1987 343 349
10. X. Chen, P. Reddanna, R. Reddy, R. Kidd, G. Hildenbrandt, and C. Rdddy Expression, purification, and characterization of a recombinant 5-lipoxygenase from potato tuber Biochem. Biophys. Res. Commun. 243 1998 438 443
11. M. Nakamura, T. Matsmoto, M. Noguchi, I. Yamashita, and M. Noma Expression of a cDNA encoding human 5-lipoxygenase under control of the STA1 promoter in Saccharomyces cerevisiae Gene 89 1990 231 237
12. 3+ at purine of ATP as studied by NMR J. Inorg. Biochem. 83 2001 101 105
13. R.A.F. Dixon, R.E. Diehl, E. Opas, E. Rands, P.J. Vickers, J.F. Evans, J.W. Gillard, and D.K. Miller Requirement of a 5-lipoxygenase-activating protein for leukotriene synthesis Nature 343 1990 282 284
14. O. Werz, J. Klemm, B. Samuelsson, and O. Radmark 5-Lipoxygenase is phosphorylated by p38 kinase-dependent MAPKAP kinases Proc. Natl. Acad. Sci. USA 97 2000 5261 5266
15. M. Peters-Golden, and T.G. Brock Intracellular compartmentalization of leukotriene biosynthesis Am. J. Respir. Crit. Care Med. 161 2000 S36 S40
16. J.M. Balcarek, T.W. Theisen, M.N. Cook, A. Varrichio, S.M. Hwang, and M.W. Strohsaker Isolation and characterization of a cDNA clone encoding rat 5-lipoxygenase J. Biol. Chem. 263 1988 13937 13941