Drug-induced conformational changes in multidrug efflux transporter AcrB from Haemophilus influenzae

Journal of Bacteriology

Volumn 189, Issue 15, 2007, Pages 5550-5558

  Dastidar, Vishakha ^a   Mao, Weimin ^b,d   Lomovskaya, Olga ^c   Zgurskaya, Helen I ^a  

^a UNIVERSITY OF OKLAHOMA   (United States)

^b Microcide Pharmaceuticals   (United States)

^c Mpex Pharmaceuticals Inc   (United States)

^d PFIZER INC   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

5 MALEIMIDOFLUORESCEIN; BACTERIAL PROTEIN; CYSTEINE; FLUORESCEIN; HYBRID PROTEIN; MALEIMIDE DERIVATIVE; MEMBRANE PROTEIN; PROTEIN ACRB; THIOL DERIVATIVE; TOLC PROTEIN; UNCLASSIFIED DRUG;

ARTICLE; CONFORMATIONAL TRANSITION; CONTROLLED STUDY; CYTOPLASM; ESCHERICHIA COLI; GRAM NEGATIVE BACTERIUM; HAEMOPHILUS INFLUENZAE; HYDROPHILICITY; MOLECULAR RECOGNITION; MUTATION; NONHUMAN; OUTER MEMBRANE; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTEIN FAMILY; RESPIRATORY TRACT DISEASE;

AMINO ACID SUBSTITUTION; BACTERIAL OUTER MEMBRANE PROTEINS; BACTERIAL PROTEINS; CYSTEINE; DRUG RESISTANCE, MULTIPLE, BACTERIAL; ESCHERICHIA COLI; ESCHERICHIA COLI PROTEINS; FLUORESCEINS; HAEMOPHILUS INFLUENZAE; MEMBRANE TRANSPORT PROTEINS; MUTAGENESIS, SITE-DIRECTED; MUTATION, MISSENSE; PROTEIN CONFORMATION;

ANIMALIA; ESCHERICHIA COLI; HAEMOPHILUS INFLUENZAE; NEGIBACTERIA;

EID: 34547621067     PISSN: 00219193     EISSN: None     Source Type: Journal    
DOI: 10.1128/JB.00471-07     Document Type: Article

References (27)

1. Adler, J., and E. Bibi. 2004. Determinants of substrate recognition by the Escherichia coli multidrug transporter MdfA identified on both sides of the membrane. J. Biol. Chem. 279:8957-8965.
2. Aires, J. R., and H. Nikaido. 2005. Aminoglycosides are captured from both periplasm and cytoplasm by the AcrD multidrug efflux transporter of Escherichia coli. J. Bacteriol. 187:1923-1929.
3. Borges-Walmsley, M. I., J. Beauchamp, S. M. Kelly, K. Jumel, D. Candlish, S. E. Harding, N. C. Price, and A. R. Walmsley. 2003. Identification of oligomerization and drug-binding domains of the membrane fusion protein EmrA. J. Biol. Chem. 278:12903-12912.
4. Dodd, J. R., and D. L. Christie. 2005. Substituted cysteine accessibility of the third transmembrane domain of the creatine transporter: defining a transport pathway. J. Biol. Chem. 280:32649-32654.
5. Elkins, C. A., and H. Nikaido. 2002. Substrate Specificity of the RND-type multidrug efflux pumps AcrB and AcrD of Escherichia coli is determined predominately by two large periplasmic loops. J. Bacteriol. 184:6490-6498.
6. Lau, S. Y., and H. I. Zgurskaya. 2005. Cell division defects in Escherichia coli deficient in the multidrug efflux transporter AcrEF-TolC. J. Bacteriol. 187: 7815-7825.
7. Li, X. Z., and H. Nikaido. 2004. Efflux-mediated drug resistance in bacteria. Drugs 64:159-204.
8. Lomovskaya, O., and K. A. Bostian. 2006. Practical applications and feasibility of efflux pump inhibitors in the clinic - a vision for applied use. Biochem. Pharmacol. 71:910-918.
9. Lomovskaya, O., M. S. Warren, A. Lee, J. Galazzo, R. Fronko, M. Lee, J. Blais, D. Cho, S. Chamberland, T. Renau, R. Leger, S. Hecker, W. Watkins, K. Hoshino, H. Ishida, and V. J. Lee. 2001. Identification and characterization of inhibitors of multidrug resistance efflux pumps in Pseudomonas aeruginosa: novel agents for combination therapy. Antimicrob. Agents Chemother. 45:105-116.
10. Mao, W., M. S. Warren, D. S. Black, T. Satou, T. Murata, T. Nishino, N. Gotoh, and O. Lomovskaya. 2002. On the mechanism of substrate specificity by resistance nodulation division (RND)-type multidrug resistance pumps: the large periplasmic loops of MexD from Pseudomonas aeruginosa are involved in substrate recognition. Mol. Microbiol. 46:889-901.
11. Markham, P. N., and A. A. Neyfakh. 2001. Efflux-mediated drug resistance in gram-positive bacteria. Curr. Opin. Microbiol. 4:509-514.
12. Middlemiss, J. K., and K. Poole. 2004. Differential impact of MexB mutations on substrate selectivity of the MexAB-OprM multidrug efflux pump of Pseudomonas aeruginosa. J. Bacteriol. 186:1258-1269.
13. Misra, R., and P. R. Reeves. 1987. Role of micF in the tolC-mediated regulation of OmpF, a major outer membrane protein of Escherichia coli K-12. J. Bacteriol. 169:4722-4730.
14. Miyamae, S., O. Ueda, F. Yoshimura, J. Hwang, Y. Tanaka, and H. Nikaido, 2001. A MATE family multidrug efflux transporter pumps out fluoroquinolones in Bacteroides thetaiotaomicron. Antimicrob. Agents Chemother. 45:3341-3346.
15. +-coupled multidrug transporter from Escherichia coli, reveals a hydrophobic pathway for solutes. J. Biol. Chem. 274:19480-19486.
16. Murakami, S., R. Nakashima, E. Yamashita, T. Matsumoto, and A. Yamaguchi. 2006. Crystal structures of a multidrug transporter reveal a functionally rotating mechanism. Nature 443:173-179.
17. Murakami, S., R. Nakashima, E. Yamashita, and A. Yamaguchi. 2002. Crystal structure of bacterial multidrug efflux transporter AcrB. Nature 419:587-593.
18. Poole, K. 2004. Efflux-mediated multiresistance in gram-negative bacteria. Clin. Microbiol. Infect. 10:12-26.
19. Sanchez, L., W. Pan, M. Vinas, and H. Nikaido. 1997. The acrAB homolog of Haemophilus influenzae codes for a functional multidrug efflux pump. J. Bacteriol. 179:6855-6857.
20. Schlor, S., A. Schmidt, E. Maier, R. Benz, W. Goebel, and I. Gentschev. 1997. In vivo and in vitro studies on interactions between the components of the hemolysin (HlyA) secretion machinery of Escherichia coli. Mol. Gen. Genet. 256:306-319.
21. Seeger, M. A., A. Schiefner, T. Eicher, F. Verrey, K. Diederichs, and K. M. Pos. 2006. Structural asymmetry of AcrB trimer suggests a peristaltic pump mechanism. Science 313:1295-1298.
22. Tikhonova, E. B., V. K. Devroy, S. Y. Lau, and H. I. Zgurskaya. 2007. Reconstitution of the Escherichia coli macrolide transporter: the periplasmic membrane fusion protein MacA stimulates the ATPase activity of MacB. Mol. Microbiol.
23. Tikhonova, E. B., Q. Wang, and H. I. Zgurskaya. 2002. Chimeric analysis of the multicomponent multidrug efflux transporters from gram-negative bacteria. J. Bacteriol. 184:6499-6507.
24. Trepod, C. M., and J. E. Mott. 2004. Identification of the Haemophilus influenzae tolC gene by susceptibility profiles of insertionally inactivated efflux pump mutants. Antimicrob. Agents Chemother. 48:1416-1418.
25. Yu, E. W., J. R. Aires, G. McDermott, and H. Nikaido. 2005. A periplasmic drug-binding site of the AcrB multidrug efflux pump: a crystallographic and site-directed mutagenesis study. J. Bacteriol. 187:6804-6815.
26. Yu, E. W., G. McDermott, H. I. Zgurskaya, H. Nikaido, and D. E. Koshland, Jr. 2003. Structural basis of multiple drug-binding capacity of the AcrB multidrug efflux pump. Science 300:976-980.
27. Zgurskaya, H. I. 2002. Molecular analysis of efflux pump-based antibiotic resistance. Int. J. Med. Microbiol. 292:95-105.