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note
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4, and the solvent removed to give a solid mass. Column chromatography of the residue over silica gel using increasing concentration of chloroform in petroleum ether yielded 3h as white solid (154 mg, 77%).
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2 solution followed by washing with distilled water. Seeds (42) of the same type were placed on each of the above Petri dishes in horizontal manner; otherwise their growth could be affected. The Petri dishes were placed in greenhouse in which the temperature varied from ∼15 °C (night) to ∼30 °C (day). Level of water was maintained at the 15 mL mark of each Petri dish before start of the days experiment to compensate the water loss. Readings of the shoot and root growths were measured in millimeter scale on day 5.
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