Influence of solubility-enhancing fusion proteins and organic solvents on the in vitro biocatalytic performance of the carotenoid cleavage dioxygenase AtCCD1 in a micellar reaction system

Applied Microbiology and Biotechnology

Volumn 75, Issue 4, 2007, Pages 829-836

  Schilling, Martin ^a   Patett, Frauke ^a   Schwab, Wilfried ^b   Schrader, Jens ^a  

^a KARL WINNACKER INSTITUT   (Germany)

^b TECHNICAL UNIVERSITY OF MUNICH   (Germany)

Author keywords

Carotenoid cleavage dioxygenase; Fusion protein; Hydrophobic substrate; Micellar reaction system; Natural flavours

Indexed keywords

BIOCATALYSTS; ORGANIC SOLVENTS; REACTION KINETICS; SPECTROPHOTOMETERS;

CAROTENOID CLEAVAGE DIOXYGENASE; FUSION PROTEIN; HYDROPHOBIC SUBSTRATE; MICELLAR REACTION SYSTEM; NATURAL FLAVORS;

PROTEINS;

ALKANOL; CARRIER PROTEIN; DIOXYGENASE; HYBRID PROTEIN; ORGANIC SOLVENT; WATER;

CAROTENOID; CLEAVAGE; ENZYME ACTIVITY; HERB; ORGANIC MATTER; PROTEIN; SOLUBILITY; SOLVENT;

ARABIDOPSIS; ARTICLE; BIOCATALYST; CONCENTRATION (PARAMETERS); CORRELATION ANALYSIS; CULTURE MEDIUM; ENZYME ACTIVATION; ENZYME ACTIVITY; GAS CHROMATOGRAPHY; IN VITRO STUDY; MASS SPECTROMETRY; MICELLE; MOLECULAR MODEL; NONHUMAN; PROTEIN DEGRADATION; SOLUBILITY; SPECTROPHOTOMETRY;

ARABIDOPSIS; ARABIDOPSIS PROTEINS; DIOXYGENASES; ENZYME ACTIVATION; ESCHERICHIA COLI PROTEINS; GENE EXPRESSION; GLUTATHIONE TRANSFERASE; KINETICS; OCTOXYNOL; PEPTIDE ELONGATION FACTORS; PROTEIN ENGINEERING; RECOMBINANT FUSION PROTEINS; SOLUBILITY; SOLVENTS; SPECTROPHOTOMETRY; TRANSCRIPTION FACTORS;

ARABIDOPSIS THALIANA;

EID: 34547413967     PISSN: 01757598     EISSN: None     Source Type: Journal    
DOI: 10.1007/s00253-007-0878-z     Document Type: Article

References (26)

1. Auldridge ME, McCarty DR, Klee HJ (2006) Plant carotenoid cleavage oxygenases and their apocarotenoid products. Curr Opin Plant Biol 9:315-321
2. Aziz S, Wu Z, Robinson DS (1999) Potato lipoxygenase catalysed co-oxidation of beta-carotene. Food Chem 64:227-230
3. Bouvier F, Suire C, Mutterer J, Camara B (2003) Oxidative remodeling of chromoplast carotenoids: identification of the carotenoid dioxygenase CsCCD and CsZCD genes involved in crocus secondary metabolite biogenesis. Plant Cell 15:47-62
4. Cabrita LD, Dai W, Bottomley SP (2006) A family of E. coli expression vectors for laboratory scale and high throughput soluble protein production. BMC Biotechnol 6:12
5. Camara B, Bouvier F (2004) Oxidative remodeling of plastid carotenoids. Arch Biochem Biophys 430(1):16-21
6. Johnson JE, Cornell RB (1999) Amphitropic proteins: regulation by reversible membrane interactions (review). Mol Membr Biol 16(3):217-235
7. Kloer DP, Ruch S, Al-Babili S, Beyer P, Schulz GE (2005) The structure of a retinal-forming carotenoid oxygenase. Science 308(5719):267-269
8. Korf U, Kohl T, van der Zandt H, Zahn R, Schleeger S, Ueberle B, Wandschneider S, Bechtel S, Schnolzer M, Ottleben H, Wiemann S, Poustka A (2005) Large-scale protein expression for proteome research. Proteomics 5(14):3571-3580
9. Meziani A, Touraud D, Zradba A, Pulvin S, Pezron I, Clausse M, Kunz W (1997) Comparison of enzymatic activity and nanostructures in water/ethanol/Brij 35 and water/1-pentanol/Brij 35 systems. J Phys Chem B 101:3620-3625
10. Mordi RC, Walton JC, Burton GW, Hughes L, Ingold KU, Lindsay DA, Moffatt DJ (1993) Oxidative degradation of beta-carotene and beta-Apo-8′- carotenal. Tetrahedron 49(4):911-928
11. Nomine Y, Ristriani T, Laurent C, Lefevre JF, Weiss E, Trave G (2001) A strategy for optimizing the monodispersity of fusion proteins: application to purification of recombinant HPV E6 oncoprotein. Protein Eng 14(4):297-305
12. Olson JA, Hayaishi O (1965) The enzymatic cleavage of beta-carotene into vitamin A by soluble enzymes of rat liver and intestine. Proc Natl Acad Sci USA 54(5):1364-1370
13. Schiffer CA, Dötsch V (1996) The role of protein-solvent interactions in protein unfolding. Curr Opin Biotechnol 7(4):428-432
14. Schirmer C, Liu Y, Touraud D, Meziani A, Pulvin S, Kunz W (2002) Horse liver alcohol dehydrogenase as a probe for nanostructuring effects of alcohols in water/nonionic surfactant systems. J Phys Chem B 106:7414-7421
15. Schmidt H, Kurtzer R, Eisenreich W, Schwab W (2006) The carotenase AtCCD1 from Arabidopsis thaliana is a dioxygenase. J Biol Chem 281(15):9845-9851
16. Schwartz SH, Tan BC, Gage DA, Zeevaart JAD, McCarty DR (1997) Specific oxidative cleavage of carotenoids by VP14 of maize. Science 276:1872-1874
17. Schwartz SH, Qin X, Zeevaart JAD (2001) Characterization of a novel carotenoid cleavage dioxygenase from plants. J Biol Chem 27:25208-25211
18. Schwartz SH, Qin X, Loewen MC (2004) The biochemical characterization of two carotenoid cleavage enzymes from Arabidopsis indicates that a carotenoid-derived compound inhibits lateral branching. J Biol Chem 279(45):46940-46945
19. Shih YP, Kung WM, Chen JC, Yeh CH, Wang AH, Wang TF (2002) High-throughput screening of soluble recombinant proteins. Protein Sci 11(7):1714-1719
20. Tan BC, Joseph LM, Deng WT, Liu L, Li QB, Cline K, McCarty DR (2003) Molecular characterization of the Arabidopsis 9-cis epoxycarotenoid dioxygenase gene family. Plant J 35(1):44-56
21. Tomsic M, Bester-Rogac M, Jamnik A, Kunz W, Touraud D, Bergmann A, Glatter O (2006) Ternary systems of nonionic surfactant Brij 35, water and various simple alcohols: structural investigations by small-angle X-ray scattering and dynamic light scattering. J Colloid Interface Sci 294(1):194-211
22. Waché Y, Bosser-DeRatuld A, Mai Ly H, Belin JM (2002) Co-oxidation of beta-carotene in biphasic media. J Mol Catal B Enzym 19-20:197-201
23. Wehbi H, Feng J, Roberts MF (2003) Water-miscible organic cosolvents enhance phosphatidylinositol-specific phospholipase C phosphotransferase as well as phosphodiesterase activity. Biochim Biophys Acta 1613(1-2):15-27
24. Winterhalter P, Rouseff R (2002) Carotenoid-derived aroma compounds: an introduction. ACS Symp Ser 802:1-17
25. Wu Y, Roberts MF (1997) Phosphatidylinositol-specific phospholipase C cyclic phosphodiesterase activity depends on solvent polarity. Biochemistry 36(28):8514-8521
26. Zorn H, Langhoff S, Schreibner M, Nimtz M, Berger RG (2003) A peroxidase from Lepista irina cleaves β-carotene to flavor compounds. Biol Chem 384:1049-1056