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Volumn 276, Issue 5320, 1997, Pages 1872-1874

Specific oxidative cleavage of carotenoids by VP14 of maize

Author keywords

[No Author keywords available]

Indexed keywords

ABSCISIC ACID; CAROTENOID; VEGETABLE PROTEIN;

EID: 1842338033     PISSN: 00368075     EISSN: None     Source Type: Journal    
DOI: 10.1126/science.276.5320.1872     Document Type: Article
Times cited : (764)

References (33)
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    • (1983) Biosynthesis of Isoprenoid Compounds , pp. 437-462
    • Bu'Lock, J.D.1
  • 2
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    • G. Wald, Science 162, 230 (1968).
    • (1968) Science , vol.162 , pp. 230
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    • K. W. Foster et al., Nature 311, 756 (1984).
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  • 15
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    • W. J. Davies and H. G. Jones, Eds. Bios Scientific, Oxford
    • I. B. Taylor, in Abscisic Acid, Physiology and Biochemistry, W. J. Davies and H. G. Jones, Eds. (Bios Scientific, Oxford, 1991), pp. 23-37.
    • (1991) Abscisic Acid, Physiology and Biochemistry , pp. 23-37
    • Taylor, I.B.1
  • 18
    • 1842323667 scopus 로고    scopus 로고
    • note
    • 4, catalase (1 mg/ml), and VP14 protein. Assays were incubated for 15 min at 22° to 24°C in a total volume of 100 μl. The appropriate substrate was added in 3 μl of ethanol. The enzyme assay and all subsequent procedures were performed under red light to minimize photo-oxidation of the precursors and products.
  • 19
    • 1842315953 scopus 로고    scopus 로고
    • note
    • 25 product from violaxanthin was 382.2501, with an error of -1.8 ppm.
  • 21
    • 1842354688 scopus 로고    scopus 로고
    • note
    • 3+ + 10 mM ascorbate, 432 ± 8.4.
  • 23
    • 1842314097 scopus 로고    scopus 로고
    • note
    • 2) has a theoretical mass of 234.1620; and the experimentally determined mass was 234.1611, with an error of -3.7 ppm.
  • 24
    • 0030606607 scopus 로고    scopus 로고
    • T. Kaneko, et al., DNA Res. 3, 109 (1996).
    • (1996) DNA Res. , vol.3 , pp. 109
    • Kaneko, T.1
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    • P. J. Lea, Ed. Academic Press, New York
    • A. D. Parry, in Methods in Plant Biochemistry, P. J. Lea, Ed. (Academic Press, New York, 1993), vol. 9, pp. 381-402.
    • (1993) Methods in Plant Biochemistry , vol.9 , pp. 381-402
    • Parry, A.D.1
  • 31
    • 1842346736 scopus 로고    scopus 로고
    • note
    • 2O was added to the reactions. The reaction products were then partitioned three times into an equal volume of ethyl acetate with vigorous stirring on a vortex mixer. The ethyl acetate fractions were pooled and dried, and methyl ABA was added as an injection standard. The samples were stored under Ar at -80°C until analysis. Samples were injected on a μPorasil column (Waters) (30 by 0.4 cm) equilibrated with 90% hexane and 10% ethyl acetate. The column was eluted with a linear gradient to 20% hexane and 80% ethyl acetate over 17 min. A standard curve was constructed by injecting known quantities of the compounds analyzed and integrating the peak areas. The integrated peak area of methyl ABA was used to correct for variations in injections. The correlation coefficients were generally greater than 0.99.
  • 32
    • 1842324621 scopus 로고    scopus 로고
    • note
    • The carotenoid substrates were isolated from spinach leaves (30). The abal-5 mutant of Arabidopsis thaliana was used as a source of all-trans-zeaxanthin, which was isomerized with iodine (22) and re-chromatographed to isolate 9-cis-zeaxanthin.
  • 33
    • 1842306497 scopus 로고    scopus 로고
    • note
    • Supported by U.S. DOE grant DE-FGO2-91ER20021, NSF grant IBN-9118377 (J.A.D.Z.), NIH grant RR-00480 to the MSU Mass Spectrometry Facility (D.A.G), and NSF grant IBN-9406692 (D.R.M).


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.