A Novel Approach to Analyze Membrane Proteins by Laser Mass Spectrometry: From Protein Subunits to the Integral Complex

Journal of the American Society for Mass Spectrometry

Volumn 18, Issue 8, 2007, Pages 1429-1438

  Morgner, Nina ^a   Kleinschroth, Thomas ^a   Barth, Hans Dieter ^a   Ludwig, Bernd ^a   Brutschy, Bernhard ^a  

^a GOETHE UNIVERSITY   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

HYDROPHOBIC PROTEIN COMPLEXES; INTEGRAL COMPLEX; IR-LASER DESORBED; LASER INDUCED LIQUID BEAD ION DESORPTION; LASER MASS SPECTROMETRY; MICRODROPLETS;

DETERGENTS; HYDROPHOBICITY; INFRARED RADIATION; MASS SPECTROMETRY; PARAMETER ESTIMATION; POLYPEPTIDES;

PROTEINS;

CALNEXIN; CYTOCHROME C OXIDASE; MEMBRANE PROTEIN; PROTEIN SUBUNIT; SUCCINATE DEHYDROGENASE (UBIQUINONE); UBIQUINOL CYTOCHROME C REDUCTASE;

ARTICLE; CALIBRATION; COMPLEX FORMATION; DESORPTION; GAS; HYDROPHOBICITY; ISOELECTRIC POINT; MASS SPECTROMETRY; PARACOCCUS DENITRIFICANS; POLYACRYLAMIDE GEL ELECTROPHORESIS; PROTEIN ANALYSIS; PROTEIN QUATERNARY STRUCTURE; SOIL MICROFLORA; SOLUBILIZATION;

ELECTRON TRANSPORT COMPLEX III; ELECTRON TRANSPORT COMPLEX IV; MASS SPECTROMETRY; MEMBRANE PROTEINS; MODELS, MOLECULAR; PARACOCCUS DENITRIFICANS; PROTEIN SUBUNITS;

BACTERIA (MICROORGANISMS); PARACOCCUS DENITRIFICANS;

EID: 34447643582     PISSN: 10440305     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jasms.2007.04.013     Document Type: Article

References (51)

1. Heijne G. v. Membrane-Protein Topology. Nat. Rev. Mol. Cell. Biol 7 (2006) 909-918
2. Fenn J.B., Mann M., Meng C.K., Wong S.F., and Whitehouse C.M. Electrospray Ionization for Mass Spectrometry of Large Biomolecules. Science 246 (1989) 64-71
3. Tanaka K., Waki H., Ido Y., Akita S., Yoshida Y., and Yoshida T. Protein and Polymer Analyses up to m/z 100 000 by Laser Ionization Time-of-Flight Mass Spectrometry. Rapid Commun. Mass Spectrom 2 (1988) 151-153
4. Sobott F., and Robinson C.V. Protein Complexes Gain Momentum. Curr. Opin. Struct. Biol 12 (2002) 729-734
5. Miranker A.D. Protein Complexes and Analysis of Their Assembly by Mass Spectrometry. Curr. Opin. Struct. Biol 10 (2000) 601-606
6. Loo J.A. Studying Noncovalent Protein Complexes by Electrospray Ionization Mass Spectrometry. Mass Spectrom. Rev 16 (1997) 1-23
7. Rostom A.A., Fucini P., Benjamin D.R., Juenemann R., Nierhaus K.H., Hartl F.U., Dobson C.M., and Robinson C.V. Detection and Selective Dissociation of Intact Ribosomes in a Mass Spectrometer. Proc. Natl. Acad. Sci. U.S.A 97 (2000) 5185-5190
8. Fitzgerald M.C., Chernushevich I., Standing K.G., Whitman C.P., and Kent S.B. Probing the Oligomeric Structure of an Enzyme by Electrospray Ionization Time-of-Flight Mass Spectrometry. Proc. Natl. Acad. Sci. U.S.A 93 (1996) 6851-6856
9. Loo R.R.O., Dales N., and Andrews P.C. Surfactant Effects on Protein -Structure Examined by Electrospray-Ionization Mass-Spectrometry. Protein Sci 3 (1994) 1975
10. Rundlett K.L., and Armstrong D.W. Mechanism of Signal Suppression by an Ionic Surfactant in Capillary Electrophoresis Electrospray Ionization Mass Spectrometry. Anal. Chem 68 (1996) 3493-3497
11. Whitelegge J.P., Gomez S.M., and Faull K.F. Proteomics of Membrane Proteins. Adv. Protein Chem 65 (2003) 271-307
12. Whitelegge J.P. Tandem Mass Spectrometry of Integral Membrane Proteins for Top-down Proteomics. TrAC, Trends Anal. Chem 24 (2005) 576-582
13. Whitelegge J.P., Le Coutre J., Lee J.C., Engel C.K., Privè G.G., Faull K.F., and Kaback H.R. Toward the Bilayer Proteome, Electrospray Ionization-Mass Spectrometry of Large, Intact Transmembrane Proteins. Proc. Natl. Acad. Sci. U.S.A 96 (1999) 10695-10698
14. Hufnagel P., Schweiger U., Eckerskorn C., and Oesterhelt D. Electrospray Ionization Mass Spectrometry of Genetically and Chemically Modified Bacteriorhodopsins. Anal. Biochem 243 (1996) 46-54
15. Caroll J., Fearnley I.M., and Walker J.E. Definition of the Mitochondrial Proteome by Measurements of Molecular Masses of Membrane Proteins. Proc. Natl. Acad. Sci. U.S.A 103 (2006) 16170-16175
16. Cohen S.L., and Chait B.T. Mass Spectrometry of Whole Proteins Eluted from Sodium Dodecyl Sulfate-Polyacrylamide Gel Electrophoresis Gels. Anal. Biochem 247 (1997) 257-267
17. Schindler P.A., Van Dorsselaer A., and Falick A.M. Analysis of Hydrophobic Proteins and Peptides by Electrospray Ionization Mass Spectrometry. Anal. Biochem 213 (1993) 256-263
18. Ghaim J.B., Tsatsos P.H., Katsonouri A., Mitchell D.M., Salcedo-Hernandez R., and Gennis R.B. Matrix-Assisted Laser Desorption Ionization Mass Spectrometry of Membrane Proteins: Demonstration of a Simple Method to Determine Subunit Molecular Weights of Hydrophobic Subunits. Biochim. Biophys. Acta 1330 (1997) 113-120
19. Morgner N., Barth H.-D., and Brutschy B. A New Way to Detect Noncovalently Bonded Complexes of Biomolecules from Liquid Micro-Droplets by Laser Mass Spectrometry. Aust. J. Chem 59 (2006) 109-114
20. Sobott F., Schunk S.A., Schüth F., and Brutschy B. Examination of Condensation Products of Group 4 Alkoxides with Laser-Induced Liquid Beam Ionization/Desorption Mass Spectrometry. Chem. Eur. J 4 (1998) 2353-2359
21. Kleinekofort W., Avdiev J., and Brutschy B. A New Method of Laser Desorption Mass Spectrometry for the Study of Biological Macromolecules. Int. J. Mass Spectrom. Ion Processes 152 (1996) 135-142
22. Kleinekofort W., Pfenninger A., Plomer T., Griesinger C., and Brutschy B. Observation of Noncovalent Complexes Using Laser-induced Liquid Beam Ionization/Desorption. Int. J. Mass Spectrom. Ion Processes 156 (1996) 195-202
23. Kleinekofort W., Schweitzer M., Engels J., and Brutschy B. Analysis of Double-stranded Oligonucleotides by Laser-induced Liquid Beam Mass Spectrometry. Int. J. Mass Spectrom. Ion Processes 163 (1997) L1-L4
24. Baker S.C., Ferguson S.J., Ludwig B., Page M.D., Richter O.-M.H., and van Spanning R.J.M. Molecular Genetics of the Genus Paracoccus-Metabolically Versatile Bacteria with Bioenergetic Flexibility. Microbiol. Mol. Biol. Rev 62 (1998) 1046-1078
25. 552 from Paracoccus denitrificans in the Oxidized and Reduced States. J. Mol. Biol 295 (2000) 667-678
26. Iwata S., Ostermeier C., Ludwig B., and Michel H. Structure at 2.8 Å Resolution of Cytochrome c Oxidase from Paracoccus denitrificans. Nature 376 (1995) 660-669
27. Charvat A., Bogehold A., and Abel B. Time-resolved Micro Liquid Desorption Mass Spectrometry: Mechanism, Features, and Kinetic Applications. Aust. J. Chem 59 (2006) 81-103
28. Kohno J.Y., Toyama N., Buntine M.A., Mafune F., and Kondow T. Gas Phase Ion Formation from a Liquid Beam of Arginine in Aqueous Solution by IR Multiphoton Excitation. Chem. Phys. Lett 420 (2006) 18-23
29. 552, and Its Truncated and Site-directed Mutants. Biochemistry 41 (2002) 10629-10634
30. 1 Complex in Paracoccus denitrificans. Eur. J. Biochem 255 (1998) 100-106
31. 1 Complex from Paracoccus denitrificans: Evidence for Protonation Reactions Coupled with Quinone Binding. Biochemistry 42 (2003) 12391-12399
32. 3 from Paracoccus denitrificans and the 13-Subunit Beef Heart Enzyme. Biophys. J 60 (1991) 415-423
33. 3) from Paracoccus denitrificans. Proc. Natl. Acad. Sci. U.S.A 77 (1980) 196-200
34. 552 Acting as Electron Donor to the Paracoccus denitrificans Cytochrome c Oxidase. Biochim. Biophys. Acta 1411 (1999) 114-120
35. Franck E.U., and Deul R. Dielectric Behaviour of Methanol and Related Polar Fluids at High Pressures and Temperatures. Faraday Discuss. Chem. Soc 66 (1978) 191-198
36. Dessiaterik Y., Nguyen T., Baer T., and Miller R.E. IR Vaporization Mass Spectrometry of Aerosol Particles with Ionic Solutions: The Problem of Ion-Ion Recombination. J. Phys. Chem. A 107 (2003) 11245-11252
37. Moller J.V., and le Maire M. Detergent Binding as a Measure of Hydrophobic Surface Area of Integral Membrane Proteins. J. Biol. Chem 268 (1993) 18659-18672
38. Seddon A.M., Curnow P., and Booth P.J. Membrane Proteins, Lipids and Detergents: Not Just Soap Opera. Biochim. Biophys. Acta 1666 (2004) 105-117
39. Garavito R.M., and Ferguson-Miller S. Detergents as Tool in Membrane Biochemistry. J. Biol. Chem 276 (2001) 32403-32406
40. Richter O.-M.H., and Ludwig B. Cytochrome c Oxidase-Structure, Function, and Physiology of a Redox-driven Molecular Machine. Rev. Physiol. Biochem. Pharmacol 147 (2003) 47-74
41. Hosler J.P., Ferguson-Miller S., and Mills D.A. Energy Transduction: Proton Transfer through the Respiratory Complexes. Annu. Rev. Biochem 75 (2006) 165-187
42. Witt H., and Ludwig B. Isolation, Analysis, and Deletion of the Gene Coding for Subunit IV of Cytochrome c Oxidase in Paracoccus denitrificans. J. Biol. Chem 272 (1997) 5514-5517
43. Ludwig B., Grabo M., Gregor I., Lustig A., Regenass M., and Rosenbusch J. Solubilized Cytochrome c Oxidase from Paracoccus denitrificans is a Monomer. J. Biol. Chem 257 (1982) 5576-5578
44. 1 Complex from Paracoccus denitrificans in Solutions of Dedecyl Maltoside. Prog. Colloid Polym. Sci 119 (2002) 77-83
45. 1 Complex from the Yeast Saccharomyces cerevisiae Co-crystallized with an Antibody Fv-Fragment. Structure 8 (2000) 669-684
46. 1 Complex-Nucleotide Sequence and Homologies between Bacterial and Mitochondrial Subunits. J. Biol. Chem 262 (1987) 13805-13811
47. 1 Complex Mutant from Rhodobacter sphaeroides. Biochim. Biophys. Acta 1757 (2006) 835-840
48. 1: Comparison with Its Mitochondrial and Chloroplast Counterparts. Photosynth. Res 81 (2004) 251-275
49. Berry E.A., Guergova-Kuras M., Huang L.-S., and Crofts A.R. Structure and Function of Cytochrome bc Complexes. Annu. Rev. Biochem 69 (2000) 1005-1075
50. Stroh A., Anderka O., Pfeiffer K., Yagi T., Finel M., Ludwig B., and Schägger H. Assembly of Respiratory Complexes I, III, and IV into NADH Oxidase Supercomplex Stabilizes Complex I in Paracoccus denitrificans. J. Biol. Chem 279 (2004) 5000-5007
51. Howlett G.J., Minton A.P., and Rivas G. Analytical Ultracentrifugation for the Study of Protein Association and Assembly. Sci. Direct 10 (2006) 430-436