Generation, characterization and crystallization of a highly active and stable cytochrome bc1 complex mutant from Rhodobacter sphaeroides

Biochimica et Biophysica Acta - Bioenergetics

Volumn 1757, Issue 7, 2006, Pages 835-840

  Elberry, Maria ^a   Xiao, Kunhung ^a   Esser, Lothar ^b   Xia, Di ^b   Yu, Linda ^a   Yu, Chang An ^a  

^a OKLAHOMA STATE UNIVERSITY   (United States)

^b NATIONAL CANCER INSTITUTE   (United States)

Author keywords

Cytochrome bc1 complex

Indexed keywords

STIGMATELLIN; UBIQUINOL CYTOCHROME C REDUCTASE;

ARTICLE; CELL MUTANT; COMPARATIVE STUDY; CONTROLLED STUDY; CRYSTALLIZATION; DIFFERENTIAL SCANNING CALORIMETRY; ELECTRON TRANSPORT; ENZYME ACTIVITY; ENZYME DENATURATION; ENZYME ENGINEERING; ENZYME PURIFICATION; ENZYME STABILITY; ENZYME STRUCTURE; NONHUMAN; PRIORITY JOURNAL; RHODOBACTER SPHAEROIDES; STRUCTURE ANALYSIS; THERMODYNAMICS; WILD TYPE; X RAY CRYSTALLOGRAPHY;

CALORIMETRY, DIFFERENTIAL SCANNING; CRYSTALLIZATION; CRYSTALLOGRAPHY, X-RAY; ELECTRON TRANSPORT COMPLEX III; ELECTROPHORESIS, POLYACRYLAMIDE GEL; PROTEIN ENGINEERING; RHODOBACTER SPHAEROIDES;

BACTERIA (MICROORGANISMS); RHODOBACTER SPHAEROIDES;

EID: 33746509579     PISSN: 00052728     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.bbabio.2006.05.031     Document Type: Article

References (22)

1. Trumpower B.L., and Gennis R.B. Energy transduction by cytochrome complexes in mitochondrial and bacterial respiration: the enzymology of coupling electron transfer reactions to transmembrane proton translocation. Ann. Rev. Biochem. 63 (1994) 675-716
2. Yang X.H., and Trumpower B.L. Isolation of a three-subunit cytochrome bc1 complex from Paracoccus denitrificans. Methods Enzymol. 126 (1986) 316-325
3. Robertson D.E., Ding H., Chelminski P.R., Slaughter C., Hsu J., Moomaw C., Tokito M., Daldal F., and Dutton P.L. Hydroubiquinone-cytochrome c2 oxidoreductase from Rhodobacter capsulatus: definition of a minimal, functional isolated preparation. Biochemistry 32 (1993) 1310-1317
4. Schagger H., Brandt U., Gencic S., and Von Jagow G. Ubiquinol-cytochrome c reductase from human and bovine mitochondria. Methods Enzymol. 260 (1995) 82-96
5. Trumpower B.L. The protonmotive Q cycle. Energy transduction by coupling of proton translocation to electron transfer by the cytochrome bc1 complex. J. Biol. Chem. 265 (1990) 11409-11412
6. Mitchell P. Possible molecular mechanisms of the protonmotive function of cytochrome systems. J. Theor. Biol. 62 (1976) 327-367
7. Ljungdahl P.O., Pennoyer J.D., Robertson D.E., and Trumpower B.L. Purification of highly active cytochrome bc1 complexes from phylogenetically diverse species by a single chromatographic procedure. Biochim. Biophys. Acta 891 (1987) 227-241
8. Yu L., Tso S.C., Shenoy S.K., Quinn B.N., and Xia D. The role of the supernumerary subunit of Rhodobacter sphaeroides cytochrome bc1 complex. J. Bioenerg. Biomembranes 3 (1999) 251-257
9. Xia D., Yu C.A., Kim H., Xia J.Z., Kachurin A.M., Zhang L., Yu L., and Deinsenhofer J. Crystal structure of the cytochrome bc1 complex from bovine heart mitochondria. Science 277 (1997) 60-66
10. Esser L., Quinn B., Li Y.F., Zhang M., Elberry M., Yu L., Yu C.A., and Xia D. Crystallographic studies of quinol oxidation site inhibitors: a modified classification of inhibitors for the cytochrome bc1 complex. J. Mol. Biol. 341 (2004) 281-302
11. Huang L.S., Cobessi D., Tung E.Y., and Berry E. Binding of the respiratory chain inhibitor antimycin to the mitochondrial bc1 complex: a new crystal structure reveals an altered intramolecular hydrogen-bonding pattern. J. Mol. Biol. 351 (2005) 573-597
12. Berry E.A., Huang L.S., Saechao L.K., Ning G.P., Valkova-Valchanova M., and Daldal F. X-ray structure of Rhodobacter capsulatus cytochrome bc1: comparison with its mitochondrial and chloroplast counterparts. Photosynth. Res. 81 (2004) 251-275
13. Yu C.A., and Yu L. Synthesis of biologically active ubiquinone derivatives. Biochemistry 21 (1982) 4096-4101
14. Mather M.W., Yu L., and Yu C.A. The involvement of threonine 160 of cytochrome b of Rhodobacter sphaeroides cytochrome bc1 complex in quinone binding and interaction with subunit IV. J. Biol. Chem. 270 (1995) 28668-28675
15. Tian H., Yu L., Mather M.W., and Yu C.A. The involvement of serine 175 and alanine 185 of cytochrome b of Rhodobacter sphaeroides cytochrome bc1 complex in interaction with iron-sulfur protein. J. Biol. Chem. 272 (1997) 23722-23728
16. Tian H., Yu L., Mather M.W., and Yu C.A. Flexibility of the neck region of the Rieske iron-sulfur protein is functionally important in the cytochrome bc1 complex. J. Biol. Chem. 273 (1998) 27953-27959
17. Berden J.A., and Slater E.C. The reaction of antimycin with a cytochrome b preparation active in reconstitution of the respiratory chain. Biochim. Biophys. Acta 216 (1970) 237-249
18. Yu L., Dong J.H., and Yu C.A. Characterization of purified cytochrome c1 from Rhodobacter sphaeroides R-26. Biochim. Biophys. Acta 852 (1986) 203-211
19. Laemmli U.K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227 (1970) 680-685
20. Otwinowski Z., and Minor W. Processing of X-ray diffraction data collected in oscillation mode. Methods Enzymol. 276 (1997) 307-326
21. Xiao K., Yu L., and Yu C.A. Confirmation of the involvement of protein domain movement during the catalytic cycle of the cytochrome bc1 complex by the formation of an intersubunit disulfide bond between cytochrome b and the iron-sulfur protein. J. Biol. Chem. 275 (2000) 38597-38604
22. Elberry M., Yu L., and Yu C.A. The disulfide bridge in the head domain of Rhodobacter sphaeroides cytochrome c1 is needed to maintain its structural integrity. Biochemistry 45 (2006) 4991-4997