Mutational analysis reveals that all tailoring region genes are required for production of polyketide antibiotic mupirocin by Pseudomonas fluorescens: Pseudomonic acid B biosynthesis precedes pseudomonic acid A

Journal of Biological Chemistry

Volumn 282, Issue 21, 2007, Pages 15451-15461

  Hothersall, Joanne ^a   Wu, Ji'en ^b   Rahman, Ayesha S ^a   Shields, Jennifer A ^a   Haddock, James ^a   Johnson, Nicola ^a   Cooper, Sian M ^a   Stephens, Elton R ^a   Cox, Russell J ^b   Crosby, John ^b   Willis, Christine L ^b   Simpson, Thomas J ^b   Thomas, Christopher M ^a  

^a UNIVERSITY OF BIRMINGHAM   (United Kingdom)

^b UNIVERSITY OF BRISTOL   (United Kingdom)

Author keywords

[No Author keywords available]

Indexed keywords

MUTATIONAL ANALYSIS; OXIDATION STATES; PSEUDOMONIC ACID; TRANSCRIPTIONAL UNITS;

ADDITION REACTIONS; ANTIBIOTICS; BIOSYNTHESIS; CARBOXYLIC ACIDS; MUTAGENESIS; OXIDATION;

PROTEINS;

ACID; ACYL CARRIER PROTEIN; ACYL COA SYNTHASE; ANTIBIOTIC AGENT; BACTERIAL PROTEIN; CYTOCHROME P450; DIENOYL COA REDUCTASE; KETONE DERIVATIVE; METHYL GROUP; MONIC ACID; OXIDOREDUCTASE; POLYKETIDE; PROTEIN MUPH; PROTEIN MUPJ; PROTEIN MUPK; PSEUDOMONIC ACID; TETRAHYDROPYRAN DERIVATIVE; UNCLASSIFIED DRUG; FATTY ACID; MACROLIDE; PSEUDOMONIC ACID I;

ARTICLE; BACTERIAL GENE; BACTERIAL MUTATION; BACTERIUM MUTANT; DRUG MANUFACTURE; DRUG SYNTHESIS; GENE CLUSTER; GENE DELETION; GENETIC TRANSCRIPTION; MUTATIONAL ANALYSIS; NONHUMAN; OPEN READING FRAME; OXIDATION; PRIORITY JOURNAL; PROMOTER REGION; PSEUDOMONAS FLUORESCENS; BIOSYNTHESIS; ENZYMOLOGY; GENETICS; METABOLISM; METHYLATION; MULTIGENE FAMILY; NUCLEOTIDE SEQUENCE; OXIDATION REDUCTION REACTION;

PSEUDOMONAS FLUORESCENS;

BACTERIAL PROTEINS; BASE SEQUENCE; FATTY ACIDS; GENES, BACTERIAL; MACROLIDES; METHYLATION; MULTIGENE FAMILY; MUPIROCIN; OPEN READING FRAMES; OXIDATION-REDUCTION; OXIDOREDUCTASES; PSEUDOMONAS FLUORESCENS; SEQUENCE DELETION;

EID: 34447532448     PISSN: 00219258     EISSN: 1083351X     Source Type: Journal    
DOI: 10.1074/jbc.M701490200     Document Type: Article

References (42)

1. Hughes, J., and Mellows, G. (1978) Biochem. J. 176, 305-318
2. Chain, E. B., and Mellows, G. (1974) J. Chem. Soc. Chem. Commun. 847-848
3. Chain, E. B., and Mellows, G. (1977) J. Chem. Soc. Perkin Trans. 1, 294-309
4. Chain, E. B., and Mellows, G. (1977) J. Chem. Soc. Perkin Trans. 1, 318-322
5. Clayton, J. P., O'Hanlon, P. J., and Rogers, N. H. (1980) Tetrahedron Lett. 21, 881-884
6. O'Hanlon, P. J., Rogers, N. H., and Tyler, J. W. (1983) J. Chem. Soc. Perkin Trans. 1, 2655-2665
7. Staunton, J., and Weissmann, K. J. (2001) Nat. Prod. Rep. 18, 380-416
8. Weissman, K. J., and Leadlay, P. F. (2005) Nat. Rev. Microbiol. 3, 925-936
9. Rix, U., Fischer, C., Remsing, L. L., and Rohr, J. (2002) Nat. Prod. Rep. 19, 542-580
10. El-Sayed, A. K., Hothersall, J., Cooper, S. M., Stephens, E., Simpson, T. J., and Thomas, C. M. (2003) Chem. Biol. 10, 419-430
11. Feline, T. C., Jones, R. B., Mellows, G., and Phillips, L. (1977) J. Chem. Soc. Perkin Trans. 1, 309-318
12. Martin, F. M., and Simpson, T. J. (1989) J. Chem. Soc. Perkin Trans. 1, 207-209
13. Cooper, S. M., Cox, R. J., Crosby, J., Crump, M. P., Hothersall, J., Laosripaiboon, W., Simpson, T. J., and Thomas, C. M. (2005) Chem. Commun. 1179-1181
14. Cooper, S. M., Laosripaiboon, W., Rahman, A. S., Hothersall, J., El-Sayed, A. K., Winfield, C., Crosby, J., Cox, R. J., Simpson, T. J., and Thomas, C. M. (2005) Chem. Biol. 12, 825-833
15. Whatling, C. A., Hodgson, J. E., Burnham, M. K. R., Clarke, N. J., Franklin, F. C. H., and Thomas, C. M. (1995) Microbiology 141, 973-982
16. Hanahan, D. (1983) J. Mol. Biol. 166, 557-580
17. Simon, R., Priefer, U., and Puhler, A. (1983) Bio-Technology 1, 784-791
18. Moir, A., Lafferty, E., and Smith, D. A. (1979) J. Gen. Microbiol. 111, 165-168
19. Kahn, M., Kolter, R., Thomas, C. M., Figurski, D. H., Meyer, R., Remeut, E., and Helinski, D. R. (1979) Methods Enzymol. 68, 268-280
20. Birnboim, H. C., and Doly, J. (1979) Nucleic Acids Res. 7, 1513-1523
21. Sambrook, J., Fritsch, E. F., and Maniatis, T. (1989) Molecular Cloning: A Laboratory Manual, 2nd Ed., Cold Spring Harbor Laboratory, Cold Spring Harbor, NY
22. Cohen, S. N., Chang, A. C. Y., and Hsu, H. (1972) Proc. Natl. Acad. Sci. U. S. A. 69, 2110-2114
23. Sanger, F., Nicklen, S., and Coulson, A. R. (1977) Proc. Natl. Acad. Sci. U. S. A. 74, 4563-4567
24. Yanagisawa, T., and Kawakami, M. (2003) J. Biol. Chem. 278, 25887-25894
25. Albertini, A. M., Caramori, T., Scoffone, F., Scotti, C., and Galizzi, A. (1995) Microbiology 141, 299-309
26. Chen, X. H., Vater, J., Piel, J., Franke, P., Scholz, R., Schneider, K., Koumoutsi, A., Hitzeroth, G., Grammel, N., Strittmatter, A. W., Gottschalk, G., Sussmuth, R. D., and Borriss, R. (2006) J. Bacteriol. 188, 4024-4036
27. Butcher, R. A., Schroeder, F. C., Fischbach, M. A., Straight, P. D., Kolter, R., Walsh, C. T., and Clardy, J. (2007) Proc. Natl. Acad. Sci. U. S. A. 104, 1506-1509
28. Gu, L. C., Jia, J. Y., Liu, H. C., Hakansson, K., Gerwick, W. H., and Sherman, D. H. (2006) J. Am. Chem. Soc. 128, 9014-9015
29. Edwards, D. J., Marquez, B. L., Nogle, L. M., McPhail, K., Goeger, D. E., Roberts, M. A., and Gerwick, W. H. (2004) Chem. Biol. 11, 817-833
30. Calderone, C. T., Kowtoniuk, W. E., Kelleher, N. L., Walsh, C. T., and Dorrestein, P. C. (2006) Proc. Natl. Acad. Sci. U. S. A. 103, 8977-8982
31. Wu, J.-E., Cooper, S. M., Cox, R. J., Crosby, J., Crump, M. P., Hothersall, J., Simpson, T. J., Thomas, C. M., and Willis, C. L. (2007) Chem. Commun. 10.1039/B700613F
32. Brusilow, W. A. (1993) Mol. Microbiol. 9, 419-424
33. Rao, R., Pagan, J., and Senior, A. E. (1988) J. Biol. Chem. 263, 15957-15963
34. Humbert, R., and Altendorf, K. (1989) J. Bacteriol. 171, 1435-1444
35. Basu, A., Chatterjee, S., and Gupta, S. K. D. (2004) J. Bacteriol. 186, 335-342
36. Basu, A., Chawla-Sarkar, M., Chakrabarti, S., and Gupta, S. K. D. (2002) J. Bacteriol. 184, 2204-2214
37. Van de Casteele, M., Legrain, C., Desmarez, L., Chen, P. G., Piérard, A., and Glansdorff, N. (1997) Comp. Biochem. Physiol. 118A, 463-473
38. El-Sayed, A. K., Hothersall, J., and Thomas, C. M. (2001) Microbiology 147, 2127-2139
39. Xu, J., Wan, E., Kim, C. J., Floss, H. G., and Mahmud, T. (2005) Microbiology 151, 2515-2528
40. Zhu, L., Ostash, B., Rix, U., Nur-e-Alam, M., Mayers, A., Luzhetskyy, A., Mendez, C., Salas, J. A., Bechthold, A., Fedorenko, V., and Rohr, J. (2005) J. Org. Chem. 70, 631-638
41. Weitnauer, G., Mühlenweg, A., Trefzer, A., Hoffmeister, D., Süβmuth, R. D., Jung, G., Welzel, K., Vente, A., Girreser, U., and Bechthold, A. (2001) Chem. Biol. 8, 569-581
42. Julien, B., Tian, Z.-Q., Reid, R., and Reeves, C. D. (2006) Chem. Biol. 13, 1277-1286