Nucleocytoplasmic shuttling of the ecdysteroid receptor (EcR) and of ultraspiracle (Usp) from Drosophila melanogaster in mammalian cells: Energy requirement and interaction with exportin

Archives of Insect Biochemistry and Physiology

Volumn 65, Issue 3, 2007, Pages 134-142

  Betanska, Katarzyna ^a   Nieva, Claudia ^a,b   Spindler Barth, Margarethe ^a   Spindler, Klaus Dieter ^a,c  

^a UNIVERSITY OF ULM   (Germany)

^b INSTITUT DE BIOLOGIA MOLECULAR DE BARCELONA   (Spain)

^c Abt Allg Zool und Endokrinologie   (Germany)

Author keywords

Cycloheximide; Leptomycin B; Nuclear receptor; Oligomycin; Ran

Indexed keywords

CELL RECEPTOR; CF1 PROTEIN, INSECT; DNA BINDING PROTEIN; ECDYSONE RECEPTOR; EXPORTIN 1 PROTEIN; KARYOPHERIN; LEPTOMYCIN B; OLIGOMYCIN; STEROID RECEPTOR; TRANSCRIPTION FACTOR; UNCLASSIFIED DRUG; UNSATURATED FATTY ACID;

ACTIVE TRANSPORT; ANIMAL; CHO CELL; CONFERENCE PAPER; CRICETULUS; DROSOPHILA MELANOGASTER; ENERGY METABOLISM; HAMSTER; HELA CELL; HUMAN; METABOLISM; PHYSIOLOGY; PROTEIN BINDING;

ACTIVE TRANSPORT, CELL NUCLEUS; ANIMALS; CHO CELLS; CRICETINAE; CRICETULUS; DNA-BINDING PROTEINS; DROSOPHILA MELANOGASTER; ENERGY METABOLISM; FATTY ACIDS, UNSATURATED; HELA CELLS; HUMANS; KARYOPHERINS; OLIGOMYCINS; PROTEIN BINDING; RECEPTORS, CYTOPLASMIC AND NUCLEAR; RECEPTORS, STEROID; TRANSCRIPTION FACTORS;

DROSOPHILA MELANOGASTER; MAMMALIA;

EID: 34447515364     PISSN: 07394462     EISSN: 15206327     Source Type: Journal    
DOI: 10.1002/arch.20185     Document Type: Conference Paper

References (29)

1. Arts GJ, Fornerod M, Mattaj IW. 1998. Identification of a nuclear export receptor for tRNA. Curr Biol 12:305-314.
2. Baumann CT, Maruvada P, Hager GL, Yen PM. 2001. Nuclear cytoplasmic shuttling by thyroid hormone receptors. Multiple protein interactions are required for nuclear retention. J Biol Chem 276:237-245.
3. Bunn CF, Neidig JA, Freidinger KE, Stankiewicz TA, Weaver BS, McGrew J, Allison LA. 2001. Nucleocytoplasmic shuttling of the thyroid hormone receptor alpha. Mol Endocrinol 15:512-533.
4. Cronauer MV, Braun S, Tremmel Ch, Kröncke K-D, Spindler-Barth M. 2007. Nuclear localization and DNA binding of ecdysone Receptor and ultraspiracle. Arch Insect Biochem Physiol 65:125-133 (this issue).
5. Damelin M, Silver PA, Corbett AH, 2002. Nuclear protein transport. Methods Enzymol 351:587-607.
6. Fahrenkrog B, Aebi U. 2003. The nucleopore complex: nucleocytoplasmic transport and beyond. Nature Mol Cell Biol 4:757-766.
7. Fischer U, Huber J, Boelens WC, Mattaj IW, Luhrmann R. 1995. The HIV-1 Rev activation domain is a nuclear export signal that accesses an export pathway used by specific cellular RNAs. Cell 11:475-483.
8. Guichon-Mantel A, Lescop P, Christin-Maitre S, Loosfeldt H, Perrot-Applanat M, Milgrom E. 1991. Nucleocytoplasmic shuttling of the progesterone receptor. EMBO J 10: 3851-3859.
9. Gwozdz T, Dutko-Gwozdz J, Nieva C, Betanska K, Orlowski M, Kowalska A, Dobrucki J, Spindler-Barth M, Spindler KD, Ozyhar A. 2007. EcR and Usp, components of the ecdysteroid nuclear receptor complex, exhibit differential distribution of molecular determinants directing subcellular trafficking. Cell Signal 19:490-503.
10. Harel A, Forbes DJ. 2004. Importin beta: conducting a much larger cellular symphony. Mol Cell 16:319-339.
11. Holaska JM, Black BE, Love DC, Hanover JA, Leszyk KJ. 2001. Calreticulin is a receptor for nuclear export. J Cell Biol 152:127-140.
12. Kaffman A, Rank NM, O'Neill EM, Huang LS, O'Shea EK. 1998. The receptor Msn5 exports the phosphorylated transcription factor Pho4 out of the nucleus. Nature 396:482-486.
13. Khyse-Andersen J. 1984. Electroblotting of multiple gels: a simple apparatus without buffer tank for rapid transfer of proteins from polyacrylamide to nitrocellulose. J. Biochem Biophys Methods 10:203-209.
14. Kutay U, Guttinger S. 2005. Leucine-rich nuclear-export signals: born to be weak. Trends Cell Biol 15:121-124.
15. Kutay U, Bischoff FR, Kostka S, Kraft R, Görlich D. 1997. Export of importin alpha from the nucleus is mediated by a specific nuclear transport factor. Cell 90:1061-1071.
16. Kutay U, Lipowsky G, Izaurralde E, Bischoff FR, Schwarzmaier P, Hartmann E, Görlich D. 1998. Identification of a tRNA-specific nuclear export receptor. Mol Cell 1:359-369.
17. Laemmli UK. 1970. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 27:680-685.
18. Laudet V, Gronemeyer H. 2002. The nuclear receptors. New York: Academic Press.
19. Maruvada P, Baumann CT, Hager GL, Yen PM. 2003. Dynamic shuttling and intracellular mobility of nuclear hormone receptors. J Biol Chem 278:12425-12432.
20. Newmeyer DD, Forbes DJ. 1988. Nuclear import can be separated into distinct steps in vitro: nuclear pore binding and translocation. Cell 52:641-653.
21. Nieva C, Gwozdz T, Dutko-Gwozdz J, Wiedenmann J, Spindler-Barth M, Wieczorek E, Dobrucki J, Duś D, Henrich VC. Oæyhar A, Spindler K-D. 2005. Ultraspiracle promotes nuclear localization of ecdysteroid receptor in mammalian cells. Biol Chem 386:463-470.
22. Prüfer K, Barsony J. 2002. Retinoid X receptor dominates the nuclear import and export of the unliganded vitamin D receptor. Mol Endocrinol 16:1738-1751.
23. Richardson WD, Mills AD, Dilworth SM, Laskey RA, Dingwall C. 1988. Nuclear protein migration involves two steps: rapid binding at the nuclear envelope followed by slower translocation through nuclear pores. Cell 52:655-664.
24. Saporita AJ, Zhang Q, Navai N, Dincer Z, Hahn J, Cai X, Wang Z. 2003. Identification and characterization of a ligand-regulated nuclear export signal in androgen receptor. J Biol Chem 278:41998-42005.
25. Tyagi RK, Amazit L, Lescop P, Milgrom E, Guiochon-Mantel A. 1998. Mechanisms of progesterone receptor export from nuclei: role of nuclear localization signal, nuclear export signal, and ran guanosine triphosphate. Mol Endocrinol 12:1684-1695.
26. Weis K. 2003. Regulating access to the genome: nucleocytoplasmic transport throughout the cell cycle. Cell 112:441-451.
27. Wen W, Meinkoth JL, Tsien RY, Taylor SS. 1995. Identification of a signal for rapid export of proteins from the nucleus. Cell 82:463-473.
28. Whitaker HC, Hanrahan S, Totty N, Gamble SC, Waxman J, Cato ACB, Hurst HC, Bevan CL. 2004. Androgen receptor is targeted to distinct subcellular compartments in response to different therapeutic antiandrogens. Clin Cancer Res 10:7392-7401.
29. Yoshida M, Horinouchi S. 1999. Trichostatin and leptomycin. Inhibition of histone deacetylation and signal dependent nuclear export. Ann NY Acad Sci 886:23-36.