An immunomagnetic separation polymerase chain reaction assay for rapid and ultra-sensitive detection of Cryptosporidium parvum in drinking water

FEMS Microbiology Letters

Volumn 176, Issue 2, 1999, Pages 285-289

  Hallier Soulier, Sylvie ^a   Guillot, Emmanuelle ^a  

^a CIRSEE   (France)

Author keywords

Cryptosporidium parvum; Drinking water; Immunomagnetic separation polymerase chain reaction

Indexed keywords

ANTIBODY; DIGOXIGENIN; DRINKING WATER; PROTOZOAL DNA;

ARTICLE; CELL SEPARATION; CONTROLLED STUDY; CRYPTOSPORIDIUM PARVUM; MAGNETISM; NONHUMAN; OLIGONUCLEOTIDE PROBE; OOCYST; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; TECHNIQUE; WATER CONTAMINATION;

ANIMALS; CRYPTOSPORIDIUM PARVUM; IMMUNOLOGIC TECHNIQUES; IMMUNOMAGNETIC SEPARATION; MICROSPHERES; POLYMERASE CHAIN REACTION; SENSITIVITY AND SPECIFICITY; WATER MICROBIOLOGY; WATER SUPPLY;

CRYPTOSPORIDIUM PARVUM;

EID: 0033051958     PISSN: 03781097     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0378-1097(99)00246-3     Document Type: Article

References (14)

1. Current W.L., Garcia L.S. Cryptosporidiosis Clin. Microbiol. Rev. 4:1991;325-358.
2. Korich D.G., Mead J.R., Madore M.S., Sinclair N.A., Sterling C.R. Effects of ozone, chlorine dioxide, chlorine, and monochloramine on Cryptosporidium parvum oocyst viability. Appl. Environ. Microbiol. 56:1990;1423-1428.
3. DuPont H.L., Chappell C.L., Sterling C.R., Okhuysen P.C., Rose J.B., Jakubowski W. The infectivity of Cryptosporidium parvum in healthy volunteers. N. Engl. J. Med. 332:1995;855-859.
4. Anonymous (1991) Proposed test method for Giardia cysts and Cryptosporidium oocysts in low-turbidity water by a fluorescence procedure. In: Annual Book of the American Society for Testing and Materials, Vol. 11, pp. 925-934. Philadelphia, PA.
5. Anonymous (1997) Method 1622: Cryptosporidium in Water by Filtration/IMS/FA. EPA 821-R-97-021 United States Environmental Protection Agency, Washinton, DC.
6. Laxer M.A., Timblin B.K., Patel R.J. DNA sequences for the specific detection of Cryptosporidium parvum by the polymerase chain reaction. Am. J. Trop. Med. Hyg. 45:1991;688-694.
7. Stinear T., Matusan A., Hines K., Sander M. Detection of a single viableCryptosporidium parvum oocyst in environmental water concentrate by reverse transcription-PCR. Appl. Environ. Microbiol. 62:1996;3385-3390.
8. Johnson D.W., Pieniazek N.J., Griffin D.W., Misener L., Rose J.B. Development of a PCR protocol for sensitive detection of Cryptosporidium oocysts in water samples. Appl. Environ. Microbiol. 61:1995;3849-3855.
9. Rochelle P.A., De Leon R., Stewart M.H., Wolfe R.L. Comparison of primers and optimization of PCR conditions for detection of Cryptosporidium parvum and Giardia lamblia in water. Appl. Environ. Microbiol. 63:1997;106-114.
10. Olsvik O., Popovic T., Skjerve E., Cudjoe K.S., Hornes E., Ugelstad J., Uhlen M. Magnetic separation techniques in diagnostic microbiology. Clin. Microbiol. Rev. 7:1994;43-54.
11. Balatbat A.B., Jordan G.W., Tang Y.J., Silva J. Jr. Detection of Cryptosporidium parvum DNA in human feces by nested PCR. J. Clin. Microbiol. 34:1996;1769-1772.
12. Rose J.B., Landeen L.K., Riley K.R., Gerba C.P. Evaluation of immunofluorescence techniques for detection of Cryptosporidium oocysts and Giardia cysts from environmental samples. Appl. Environ. Microbiol. 55:1989;3189-3196.
13. Fricker C.R., Crabb J.H. Water-borne cryptosporidiosis: detection methods and treatment options. Adv. Parasitol. 40:1998;241-278.
14. Smith H.V., Rose J.B. Waterborne Cryptosporidiosis: Current status. Parasitol. Today. 14:1998;14-22.