shRNA driven by Pol II/T7 dual-promoter system effectively induce cell-specific RNA interference in mammalian cells

Biochemical and Biophysical Research Communications

Volumn 360, Issue 2, 2007, Pages 496-500

  Peng, Ying ^a   Lu, Jian Xin ^a   Shen, Xin Feng ^a  

^a WENZHOU MEDICAL UNIVERSITY   (China)

Author keywords

RNA interference; RNA polymerase II promoter; RNA polymerase III promoter; Short hairpin RNA; T7 promoter

Indexed keywords

RNA POLYMERASE II; SHORT HAIRPIN RNA;

ARTICLE; CELL SPECIFICITY; CONTROLLED STUDY; FEMALE; GENE CONTROL; GENE EXPRESSION; HUMAN; HUMAN CELL; LIVER CELL CARCINOMA; MAMMAL CELL; PRIORITY JOURNAL; PROMOTER REGION; REPORTER GENE; RNA INTERFERENCE; RNA TRANSCRIPTION; RNA VECTOR;

DNA POLYMERASE II; GENE SILENCING; HELA CELLS; HUMANS; PROMOTER REGIONS (GENETICS); RNA INTERFERENCE; RNA, SMALL INTERFERING;

MAMMALIA;

EID: 34447099856     PISSN: 0006291X     EISSN: 10902104     Source Type: Journal    
DOI: 10.1016/j.bbrc.2007.06.100     Document Type: Article

References (23)

1. Sharp P.A., and Zamore P.D. Molecular biology. RNA interference. Science 287 (2000) 2431-2433
2. Arendt C.W., Tang G., and Zilberstein A. Vector systems for the delivery of small interfering RNAs: managing the RISC. ChemBioChem 4 (2003) 1129-1136
3. Amarzguioui M., Rossi J.J., and Kim D. Approaches for chemically synthesized siRNA and vector-mediated RNAi. FEBS Lett. 579 (2005) 5974-5981
4. Elbashir S.M., Harborth J., Lendeckel W., Yalcin A., Weber K., and Tuschl T. Duplexes of 21-nucleotide RNAs mediate RNA interference in cultured mammalian cells. Nature 411 (2001) 494-498
5. Kwak Y.D., Koike H., and Sugaya K. RNA interference with small hairpin RNAs transcribed from a human U6 promoter-driven DNA vector. J. Pharmacol. Sci. 93 (2003) 214-217
6. Miyagishi M., and Taira K. U6 promoter-driven siRNAs with four uridine 3′ overhangs efficiently suppress targeted gene expression in mammalian cells. Nat. Biotechnol. 20 (2002) 497-500
7. Sui G., Soohoo C., Affar el B., Gay F., Shi Y., Forrester W.C., and Shi Y. A DNA vector-based RNAi technology to suppress gene expression in mammalian cells. Proc. Natl. Acad. Sci. USA 99 (2002) 5515-5520
8. Yu J.Y., DeRuiter S.L., and Turner D.L. RNA interference by expression of short-interfering RNAs and hairpin RNAs in mammalian cells. Proc. Natl. Acad. Sci. USA 99 (2002) 6047-6052
9. Walther W., and Stein U. Cell type specific and inducible promoters for vectors in gene therapy as an approach for cell targeting. J. Mol. Med. 74 (1996) 379-392
10. Paddison P.J., Caudy A.A., Bernstein E., Hannon G.J., and Conklin D.S. Short hairpin RNAs (shRNAs) induce sequence-specific silencing in mammalian cells. Genes Dev. 16 (2002) 948-958
11. Lieber A., Kiessling U., and Strauss M. High level gene expression in mammalian cells by a nuclear T7-phase RNA polymerase. Nucleic Acids Res. 17 (1989) 8485-8493
12. Huber B.E., Richards C.A., and Krenitsky T.A. Retroviral-mediated gene therapy for the treatment of hepatocellular carcinoma: an innovative approach for cancer therapy. Proc. Natl. Acad. Sci. USA 88 (1991) 8039-8043
13. Ido A., Nakata K., Kato Y., Nakao K., Murata K., Fujita M., Ishii N., Tamaoki T., Shiku H., and Nagataki S. Gene therapy for hepatoma cells using a retrovirus vector carrying herpes simplex virus thymidine kinase gene under the control of human alpha-fetoprotein gene promoter. Cancer Res. 55 (1995) 3105-3109
14. Clarke D.K., Sidhu M.S., Johnson J.E., and Udem S.A. Rescue of mumps virus from cDNA. J. Virol. 74 (2000) 4831-4838
15. Snove Jr. O., and Rossi J.J. Expressing short hairpin RNAs in vivo. Nat. Methods 3 (2006) 689-695
16. Xia H., Mao Q., Paulson H.L., and Davidson B.L. siRNA-mediated gene silencing in vitro and in vivo. Nat. Biotechnol. 20 (2002) 1006-1010
17. Gou D., Narasaraju T., Chintagari N.R., Jin N., Wang P., and Liu L. Gene silencing in alveolar type II cells using cell-specific promoter in vitro and in vivo. Nucleic Acids Res. 32 (2004) e134
18. Yuan J., Wang X., Zhang Y., Hu X., Deng X., Fei J., and Li N. shRNA transcribed by RNA Pol II promoter induce RNA interference in mammalian cell. Mol. Biol. Rep. 33 (2006) 43-49
19. Boden D., Pusch O., Lee F., Tucker L., Shank P.R., and Ramratnam B. Promoter choice affects the potency of HIV-1 specific RNA interference. Nucleic Acids Res. 31 (2003) 5033-5038
20. Rao M.K., Pham J., Imam J.S., MacLean J.A., Murali D., Furuta Y., Sinha-Hikim A.P., and Wilkinson M.F. Tissue-specific RNAi reveals that WT1 expression in nurse cells controls germ cell survival and spermatogenesis. Genes Dev. 20 (2006) 147-152
21. Bartel D.P. MicroRNAs: genomics, biogenesis, mechanism, and function. Cell 116 (2004) 281-297
22. Walker S.C., Avis J.M., and Conn G.L. General plasmids for producing RNA in vitro transcripts with homogeneous ends. Nucleic Acids Res. 31 (2003) e82
23. Lai M.M. The molecular biology of hepatitis delta virus. Annu. Rev. Biochem. 64 (1995) 259-286