Direct transfer and expression of human GM-CSF in tobacco suspension cell using Agrobacterium-mediated transfer system

Plant Cell, Tissue and Organ Culture

Volumn 78, Issue 2, 2004, Pages 133-138

  Kim, Young Sook ^a   Kwon, Tae Ho ^a   Sik, Yang Moon ^a  

^a Chonbuk National University   (South Korea)

Author keywords

Direct cell transformation; ELISA; hGM CSF; Suspension cell

Indexed keywords

BACTERIA; GENE TRANSFER; PROTEINS; SYNTHESIS (CHEMICAL); TOBACCO;

CELL SUSPENSIONS; TRANSGENIC CELLS;

PLANT CELL CULTURE;

AGROBACTERIUM; BACTERIA (MICROORGANISMS); NICOTIANA; NICOTIANA TABACUM; RHIZOBIUM;

EID: 3442902015     PISSN: 01676857     EISSN: None     Source Type: Journal    
DOI: 10.1023/B:TICU.0000022555.50772.09     Document Type: Article

References (17)

1. Chiou CJ & Wu MC (1990) Expression of human granulocyte-macrophage colony-stimulating factor gene in insect cells by a baculovirus vector. FEBS Lett. 259: 249-253
2. Doran PM (2000) Foreign protein production in plant tissue cultures. Curr. Opin. Biotech. 11: 199-204
3. Herbers K & Sonnewald U (1999) Production of new/modified proteins in transgenic plants. Curr. Opin. Biotechnol. 10: 163-168
4. Hoekema A, Hirsch PR, Hooykaas PJJ & Schilperoort RA (1983) A binary plant vector strategy based on separation of vir-and T-region of the Agrobacterium tumefaciens Ti-plasmid. Nature 303: 79-180
5. James EA, Eang C, Wang Z & Reeves R (2000) Production and characterization of biologically active human GM-CSF secreted by genetically modified plant cells. Protein Express. Purif. 19: 131-138
6. Kim MJ, Kwon TH, Jang YS, Yang MS & Kim DH (2000) Expression of murine GM-CSF in recombinant Aspergillus niger. J. Microbiol. Biot. 10: 287-292
7. Kim YS, Kim MY, Kwon TH & Yang MS (2003) Production of hGM-CSF from cell suspension culture of transformed lettuce using Agrobacterium-mediated transformation system. Korean J. Plant Biotech. 30: 97-102
8. Kwon TH, Seo JE, Kim J, Lee JH, Jang YS & Yang MS (2003a) Expression and secretion of the heterodimeric protein interleukin-12 in plant cell suspension culture. Biotech. Bioeng. 81: 870-875
9. Kwon TH, Shin YM, Kim YS, Jang YS & Yang MS (2003b) Secretory production of hGM-CSF with a high specific biological activity by transgenic plant cell suspension culture. Biotech. Biopro. Eng. 8: 135-141
10. Lee JS, Choi SJ, Kang HS, Oh WG, Cho KH, Kwon TH, Kim DH, Jang YS & Yang MS (1997) Establishment of the transgenic tobacco cell suspension culture system for producing murine granulocyte-macrophage colony-stimulating factor. Mol. Cells 7: 783-787
11. Libby RT, Braedt G, Kronheim SR, March CJ, Urdal DL, Chiaverotti TA, Tushinski RJ, Mochizuki DY, Hopp TP & Cosman D (1987) Expression and purification of native human granulocyte-macrophage colony-stimulating factor from an Escherichia coli secretion vector. DNA 6: 221-229
12. Miele L (1997) Plants as bioreactors for biopharmaceuticals: regulatory considerations. Trends Biotechnol. 15: 45-50
13. Murashige T & Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plant 15: 473-497
14. O'Brein IE, Baguley BC, Murray BG, Morris BA & Ferguson IB (1998) Early stage of the apoptotic pathway in plant cells are reversible. Plant J. 13: 803-814
15. Price V, Mochizuki D, March CJ, Cosman D, Deeley MC, Klinke R, Clevenger W, Gillis S, Baker P & Urdal D (1987) Expression, purification and characterization of recombinant murine granulocyte-macrophage colony-stimulating factor and bovine interleukin-2 from yeast. Gene 55: 287-293
16. Rasko JE & Gough NM (1994) Granulocyte-macrophage colony-stimulating factor. In: Thomson A (ed) The Cytokine Handbook (pp. 343-369). Academic Press, London
17. Sambrook J, Fritsch EF & Maniatis T (1989) Molecular Cloning. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY