The transmembrane domain of AtTco64 and its C-terminal lysine-rich flanking region are targeting signals to the chloroplast outer envelope membrane

Molecules and Cells

Volumn 17, Issue 2, 2004, Pages 281-291

  Lee, Yong Jik ^a   Sohn, Eun Ju ^a   Lee, Kwang Hee ^a   Lee, Dong Wok ^a   Hwang, Inhwan ^a  

^a Pohang University of Science and Technology (POSTECH)   (South Korea)

Author keywords

AtToc64; Lysine rich flanking region; Side chain of hydrophobic amino acid; Targeting to chloroplast outer envelope membrane

Indexed keywords

AMINO ACID DERIVATIVE; CHLOROPLAST RNA; DOCKING PROTEIN; LYSINE; OUTER MEMBRANE PROTEIN; PHENYLALANINE DERIVATIVE; PROTEIN ATTOC64; UNCLASSIFIED DRUG; ARABIDOPSIS PROTEIN; HYBRID PROTEIN; MEMBRANE PROTEIN; SIGNAL PEPTIDE; TOC64 PROTEIN, ARABIDOPSIS;

AMINO ACID COMPOSITION; AMINO ACID SEQUENCE; ARABIDOPSIS; ARTICLE; CARBOXY TERMINAL SEQUENCE; CELL MEMBRANE; CELL TRANSPORT; CHLOROPLAST GENETICS; CONTROLLED STUDY; CYTOSOL; DNA FLANKING REGION; GENE TARGETING; GENE TRANSLOCATION; GENETIC CODE; HYDROPHOBICITY; IN VIVO STUDY; NONHUMAN; PROTEIN DOMAIN; PROTEIN FUNCTION; PROTOPLAST; RNA TRANSLATION; SIGNAL TRANSDUCTION; CELL FRACTIONATION; CHEMISTRY; CHLOROPLAST; GENETICS; INTRACELLULAR MEMBRANE; METABOLISM; MOLECULAR GENETICS; PROTEIN TERTIARY STRUCTURE; SEQUENCE ALIGNMENT;

AMINO ACID SEQUENCE; ARABIDOPSIS PROTEINS; CHLOROPLASTS; INTRACELLULAR MEMBRANES; LYSINE; MEMBRANE PROTEINS; MOLECULAR SEQUENCE DATA; PROTEIN SORTING SIGNALS; PROTEIN STRUCTURE, TERTIARY; RECOMBINANT FUSION PROTEINS; SEQUENCE ALIGNMENT; SUBCELLULAR FRACTIONS;

EID: 3442899083     PISSN: 10168478     EISSN: None     Source Type: Journal    
DOI: 10.1016/s1016-8478(23)13039-1     Document Type: Article

References (35)

1. Bauer, J., Chen, K., Hiltbunner, A., Wehrli, E., Eugster, M., Schnell, D., and Kessler, F. (2000) The major protein import receptor of plastids is essential for chloroplast biogenesis. Nature 403, 203-207.
2. Beddoe, T. and Lithgow, T. (2000) Delivery of nascent polypeptides to the mitochondrial surface. Biochim. Biophys. Acta 1592, 35-39.
3. Beilharz, T., Egan, B., Silver, P.A., Hofmann, K., and Lithgow, T. (2003) Bipartite signals mediate subcellular targeting of tail-anchored membrane proteins in Saccharomyces cerevisiae. J. Biol. Chem. 278, 8219-8223.
4. Brandizzi, F., Frangne, N., Marc-Martin, S., Hawes, C., Neuhaus, J. M., and Paris, N. (2002) The destination for single-pass membrane proteins is influenced markedly by the length of the hydrophobic domain. Plant Cell 14, 1077-1092.
5. Chen, D. and Schnell, D. J. (1997) Insertion of the 34-kDa chloroplast protein import component, IAP34, into the chloroplast outer membrane is dependent on its intrinsic GTP-binding capacity. J. Biol. Chem. 272, 6614-6620.
6. Cline, K., Werner-Washburne, M., Lubben, T. H., and Keegstra, K. (1985) Precursors to two nuclear-encoded chloroplast proteins bind to the outer envelope membrane before being imported into chloroplasts. J. Biol. Chem. 260, 3691-3696.
7. Dammai, V. and Subramani, S. (2001) The human peroxisomal targeting signal receptor, Pex5p, is translocated into the peroxisomal matrix and recycled to the cytosol. Cell 105, 187-196.
8. Driouich, A., Zhang, G. F., and Staehelin, L. A. (1993) Effect of brefeldin A on the structure of the Golgi apparatus and on the synthesis and secretion of proteins and polysaccharides in sycamore maple (Acer pseudoplatanus) suspension-cultured cells. Plant Physiol. 101, 1363-1373.
9. George, R., Beddoe, T., Landl, K., and Lithgow, T. (1998) The yeast nascent polypeptide-associated complex initiates protein targeting to mitochondria. Proc. Natl. Acad. Sci. USA 95, 2296-2301.
10. High, S., Flint, N., and Dobberstein, B. (1991) Requirements for the membrane insertion of signal-anchor type proteins. J. Cell Biol. 113, 25-34.
11. Jarvis, P. and Soll, J. (2002) Toc, tic, and chloroplast protein import. Biochim. Biophys. Acta 1590, 177-189.
12. Jin, J. B., Kim, Y. A., Kim, S. J., Lee, S. H., Kim, D. H., Cheong, G.-W., and Hwang, I. (2001) A new dynamin-like protein, ADL6, is involved in trafficking from the trans-Golgi network to the central vacuole in Arabidopsis. Plant Cell 13, 1511-1526.
13. Kanaji, S., Iwahashi, J., Kida, Y., Sakaguchi, M., and Mihara, K. (2000) Characterization of the signal that directs Tom20 to the mitochondrial outer membrane. J. Cell Biol. 151, 277-288.
14. Keegstra, K. and Froehlich, J. E. (1999) Protein import into chloroplasts. Curr. Opin. Plant Biol. 2, 471-476.
15. Kim, D. H., Eu, Y.-J., Yoo, C. M., Kim, Y. W., Pih, K. T., Jin, J. B., Kim, S. J., Stenmark, H., and Hwang, I. (2001) Trafficking of phosphatidylinositol 3-phosphate from the trans-Golgi network to the lumen of the central vacuole in plant cells. Plant Cell 13, 287-301.
16. Kouranov, A., Wang, H., and Schnell, D. J. (1999) Tic22 is targeted to the intermembrane space of chloroplasts by a novel pathway. J. Biol. Chem. 274, 25181-25186.
17. Kyte, J. and Doolittle, R. F. (1982) A simple method for displaying the hydropathic character of a protein. J. Mol. Biol. 157, 105-132.
18. Lee, K. H., Kim, D. H., Lee, S. W., Kim, Z. H., and Hwang, I. (2002) In vivo import experiments in protoplasts reveal the importance of the overall context but not specific amino acid residues of the transit peptide during import into chloroplasts. Mol. Cells 14, 388-397.
19. Lee, Y. J., Kim, D. H., Kim, Y.-W., and Hwang, I. (2001) Identification of a signal that distinguishes between the chloroplast outer envelope membrane and the endomembrane system in vivo. Plant Cell 13, 2175-2190.
20. Li, H. M. and Chen, L. J. (1996) Protein targeting and integration signal for the chloroplastic outer envelope membrane. Plant Cell 8, 2117-2126.
21. Li, H. M., Moore, T., and Keegstra, K. (1991) Targeting of proteins to the outer envelope membrane uses a different pathway than transport into chloroplasts. Plant Cell 3, 709-717.
22. Moroianu, J. (1999) Nuclear import and export: transport factors, mechanisms and regulation. Crit. Rev. Eukaryot. Gene Exp. 9, 89-106.
23. Muckel, E. and Soll, J. (1996) A protein import receptor of chloroplast is inserted into the outer envelope membrane by a novel pathway. J. Biol. Chem. 271, 23846-23852.
24. Pedrazzini, E., Villa, A., and Borgese, N. (1996) A mutant cytochrome b5 with a lengthened membrane anchor escapes from the endoplasmic reticulum and reaches the plasma membrane. Proc. Natl. Acad. Sci. USA 93, 4207-4212.
25. Rensink, W. A., Pilon, M., and Weisbeek, P. (1998) Domains of a transit sequence required for in vivo import in Arabidopsis chloroplasts. Plant Physiol. 118, 691-699.
26. Sakaguchi, M., Mihara, K., and Sato, R. (1987) A short amino-terminal segment of microsomal cytochrome P-450 functions both as an insertion signal and as a stop-transfer sequence. EMBO J. 6, 2425-2431.
27. Salomon, M., Fischer, K., Flugge, U. I., and Soll, J. (1990) Sequence analysis and protein import studies of an outer chloroplast envelope polypeptide. Proc. Natl. Acad. Sci. USA 87, 5778-5782.
28. Schleiff, E., Tien, R., Salomon, M., and Soll, J. (2001) Lipid composition of outer leaflet of chloroplast outer envelope determines topology of OEP7. Mol. Biol. Cell 12, 4090-4102.
29. Schleiff, E., Soll, J., Kuchler, M., Kuhlbrandt, W., and Harrer, R. (2003) Characterization of the translocon of the outer envelope of chloroplasts. J. Cell Biol. 160, 541-551.
30. Schnell, D.J., Blobel, G., and Pain, D. (1990) The chloroplast import receptor is an integral membrane protein of chloroplast envelope contact sites. J. Cell Biol. 111, 1825-1838.
31. Sohrt, K. and Soll, J. (2000) Toc64, a new component of the protein translocon of chloroplasts. J. Cell Biol. 148, 1213-1221.
32. Steel, G. J., Brownsword, J., and Stirling, C. J. (2002) Tail-anchored protein insertion into yeast ER requires a novel posttranslational mechanism which is independent of the SEC machinery. Biochemistry 41, 11914-1920.
33. Tranel, P. J., Froehlich, J., Goyal, A., and Keegstra, K. (1995) A component of the chloroplastic protein import apparatus is targeted to the outer envelope membrane via a novel pathway. EMBO J. 14, 2436-2446.
34. Tu, S.-L. and Li, H. (2000) Insertion of OEP14 into the outer envelope membrane is mediated by proteinaceous components of chloroplasts. Plant Cell 12, 1951-1959.
35. Wu, C. and Ko, K. 1(993) Identification of an uncleavable targeting signal in the 70-kilodalton spinach chloroplast outer envelope membrane protein. J. Biol. Chem. 268, 19384-19391.