Flavodoxin:quinone reductase (FqrB): A redox partner of pyruvate:ferredoxin oxidoreductase that reversibly couples pyruvate oxidation to NADPH production in Helicobacter pylori and Campylobacter jejuni

Journal of Bacteriology

Volumn 189, Issue 13, 2007, Pages 4764-4773

  St Maurice, Martin ^c,f   Cremades, Nunilo ^d   Croxen, Matthew A ^a,b,c   Sisson, Gary ^c   Sancho, Javier ^d   Hoffman, Paul S ^a,b,c,e  

^a UNIVERSITY OF VIRGINIA   (United States)

^b UNIVERSITY OF VIRGINIA   (United States)

^c DALHOUSIE UNIVERSITY   (Canada)

^d UNIVERSITY OF ZARAGOZA   (Spain)

^e UNIVERSITY OF VIRGINIA HEALTH SYSTEM   (United States)

^f UNIVERSITY OF WISCONSIN MADISON   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

5,5' DITHIOBIS(2 NITROBENZOIC ACID); BENZOQUINONE; CYTOCHROME C; DNA; FLAVODOXIN; FLAVODOXIN:QUINONE REDUCTASE; MENADIONE; PYRUVATE SYNTHASE; REDUCED NICOTINAMIDE ADENINE DINUCLEOTIDE PHOSPHATE; UNCLASSIFIED DRUG;

ARTICLE; CAMPYLOBACTER JEJUNI; CARBON DIOXIDE FIXATION; CATALYSIS; CELL LYSATE; ELECTRON TRANSPORT; ENZYME MECHANISM; ENZYME SUBSTRATE; FQRB GENE; GENE; GENETIC CONSERVATION; HELICOBACTER PYLORI; LIFESTYLE; NONHUMAN; OXYGEN TENSION; PRIORITY JOURNAL;

AMINO ACID SEQUENCE; CAMPYLOBACTER JEJUNI; COMPUTATIONAL BIOLOGY; FLAVODOXIN; HELICOBACTER PYLORI; KINETICS; MOLECULAR SEQUENCE DATA; NAD(P)H DEHYDROGENASE (QUINONE); NADP; OXIDATION-REDUCTION; PYRUVATE SYNTHASE; PYRUVIC ACID; SEQUENCE HOMOLOGY, AMINO ACID; SUBSTRATE SPECIFICITY;

CAMPYLOBACTER JEJUNI; EPSILONPROTEOBACTERIA; ESCHERICHIA COLI; HELICOBACTER PYLORI;

EID: 34347386473     PISSN: 00219193     EISSN: None     Source Type: Journal    
DOI: 10.1128/JB.00287-07     Document Type: Article

References (43)

1. Alm, R. A., L. L. Ling, D. T. Moir, B. L. King, E. D. Brown, P. C. Doig, D. R. Smith, B. Noonan, B. C. Guild, B. L. deJonge, G. Carmel, P. J. Tummino, A. Caruso, M. Uria-Nickelsen, D. M. Mills, C. Ives, R. Gibson, D. Merberg, S. D. Mills, Q. Jiang, D. E. Taylor, G. F. Vovis, and T. J. Trust. 1999. Genomic sequence comparison of two unrelated isolates of the human gastric pathogen Helicobacter pylori. Nature 397:186-190.
2. Baker, L. M. S., A. Raudonikiene, P. S. Hoffman, and L. B. Poole. 2001. Essential thioredoxin-dependent peroxiredoxin system from Helicobacter pylori: genetic and kinetic characterization. J. Bacteriol. 183:1961-1973.
3. Blaser, M. J., and D. E. Berg. 2001. Helicobacter pylori genetic diversity and risk of human disease. J. Clin. Investig. 107:767-773.
4. 2 for growth. Microbiology 141:3113-3118.
5. Butzler, J.-P. 2004. Campylobacter, from obscurity to celebrity. Clin. Microbiol. Infect. 10:868-876.
6. Chalker, A. F., H. W. Minehart, N. J. Hughes, K. K. Koretke, M. A. Lonetto, K. K. Brinkman, P. V. Warren, A. Lupas, M. J. Stanhope, J. R. Brown, and P. S. Hoffman. 2001. Systematic identification of selective essential genes in Helicobacter pylori by genome prioritization and allelic replacement mutagenesis. J. Bacteriol. 183:1259-1268.
7. Chang, H. T., S. W. Marcelli, A. A. Davison, P. A. Chalk, R. K. Poole, and R. J. Miles. 1995. Kinetics of substrate oxidation by whole cells and cell membranes of Helicobacter pylori. FEMS Microbiol. Lett. 129:33-38.
8. Chen, M., L. P. Anderson, L. Zhai, and A. Kharazmi. 1999. Characterization of the respiratory chain of Helicobacter pylori. FEMS Immunol. Med. Microbiol. 24:169-174.
9. Comtois, S. L., M. D. Gidley, and D. J. Kelly. 2003. Role of the thioredoxin system and the thiol-peroxidase Tpx and Bcp in mediating resistance to oxidative and nitrosative stress in Helicobacter pylori. Microbiology 149:121-129.
10. Cremades, N., M. Bueno, M. Toja, and J. J. Sancho. 2005. Towards a new therapeutic target: Helicobacter pylori flavodoxin. Biophys. Chem. 115:267-276.
11. Doig, P., B. L. de Jounge, R. A. Alm, E. D. Brown, M. Uria-Nickelson, B. Noonan, S. D. Mills, P. Tummino, G. Carmel, B. C. Guild, D. T. Moir, G. F. Vovis, and T. J. Trust. 1999. Helicobacter pylori physiology predicted from genomic comparison of two strains. Microbiol. Mol. Biol. Rev. 63:675-707.
12. Ernst, P. B., and B. D. Gold. 2000. The disease spectrum of Helicobacter pylori: the immunopathogenesis of gastroduodenal ulcer and gastric cancer. Annu. Rev. Microbiol. 54:615-640.
13. Glupczynski, Y. 1998. Antimicrobial resistance in Helicobacter pylori: a global overview. Acta Gastroenterol. Belgium 61:357-366.
14. Goodman, T. G., and P. S. Hoffman. 1983. Hydrogenase activity in catalase-positive strains of Campylobacter spp. J. Clin. Microbiol. 18:825-829.
15. Goodwin, A., D. Kersulyte, G. Sisson, S. J. O. Veldhuyzen van Zanten, D. E. Berg, and P. S. Hoffman. 1998. Metronidazole resistance in Helicobacter pylori is due to null mutations in a gene (rdxA) that encodes an oxygen-insensitive NADPH nitroreductase. Mol. Microbiol. 28:383-393.
16. Hazell, S. L., A. G. Harris, and M. A. Trend. 2001. Evasion of the toxic effects of oxygen, p. 167-175. In H. L. T. Mobley, G. L. Mendz, and S. L. Hazell (ed.), Helicobacter pylori: physiology and genetics. ASM Press, Washington, DC.
17. Hoffman, P. S., and T. G. Goodman. 1982. Respiratory physiology and energy conservation efficiency of Campylobacter jejuni. I. Bacteriol. 150:319-326.
18. Hoffman, P. S., A. Goodwin, J. Johnsen, K. Magee, and S. J. O. Veldhuyzen van Zanten. 1996. Metabolic activities of metronidazole-sensitive and -resistant strains of Helicobacter pylori: repression of pyruvate oxidoreductase and expression of isocitrate lyase activity correlate with resistance. J. Bacteriol. 178:4822-4829.
19. Hoffman, P. S., N. R. Krieg, and R. M. Smibert. 1979. Studies of the microaerophilic nature of Campylobacter fetus subsp. jejuni. I. Physiological aspects of enhanced aerotolerance. Can, J. Microbiol. 25:1-7.
20. Hoffman, P. S., G. Sission, M. A. Croxen, K. Welch, W. D. Harman, N. Cremades, and M. G. Morash. 2007. Antiparasitic drug nitazoxanide inhibits the pyruvate oxidoreductases of Helicobacter pylori, selected anaerobic bacteria and parasites, and Campylobacter jejuni. Antimicrob. Agents Chemother. 51:868-876.
21. Horner, D. S., R. P. Hirt, and T. M. Embley. 1999. A single eubacterial origin of eukaryotic pyruvate:ferredoxin oxidoreductase genes: implications for the evolution of anaerobic eukaryotes. Mol. Biol. Evol. 16:1280-1291.
22. Hughes, N. J., P. A. Chalk, C. L. Clayton, and D. J. Kelly. 1995. Identification of carboxylation enzymes and characterization of a novel four-subunit pyruvate:flavodoxin oxidoreductase from Helicobacter pylori. J. Bacteriol. 177: 3953-3959.
23. Hughes, N. J., C. L. Clayton, P. A. Chalk, and D. J. Kelly. 1998. Helicobacter pylori porCDAB and oorDABC genes encode distinct pyruvate:flavodoxin and 2-oxoglutarate:acceptor oxidoreductases which mediate electron transport to NADP. J. Bacteriol. 180:1119-1128.
24. Jeong, J.-Y., A. K. Mukhopadhyay, D. Dailidiene, Y. Wang, B. Velapatińo, R. H. Gilman, A. J. Parkinson, G. B. Nair, B. C. Y. Wong, S. K. Lam, R. Mistry, I. Segal, Y. Yuan, H. Gao, T. Alarcon, M. L. Brea, Y. Ito, D. Kersulyte, H.-K. Lee, Y. Gong, A. Goodwin, P. S. Hoffman, and D. E. Berg. 2000. Sequential inactivation of rdxA (HP0954) and frxA (HP0642) nitroreductase genes causes moderate and high-level metronidazole resistance in Helicobacter pylori. J. Bacteriol. 182:5082-5090.
25. Jorgensen, M. A, M. A. Trend, S. L. Hazell, and G. L. Mendz. 2001. Potential involvement of several nitroreductases in metronidazole resistance in Helicobacter pylori. Arch. Biochem. Biophys. 392:180-191.
26. Koder, R. L., and A. F. Miller. 1998. Steady state kinetic mechanism, stereospecificity, substrate and inhibitor specificity of Enterobacter cloacae nitroreductase. Biochim. Biophys. Acta 1387:395-405.
27. Krieg, N. R., and P. S. Hoffman. 1986. Microaerophily and oxygen toxicity. Annu. Rev. Microbiol. 40:107-130.
28. Maier, R. J. 2005. Use of molecular hydrogen as an energy substrate by human pathogenic bacteria. Biochem. Soc. Trans. 33:83-85.
29. Marcelli, S. W., H. T. Chang, T. Chapman, P. A. Chalk, R. J. Miles, and R. K. Poole. 1996. The respiratory chain of Helicobacter pylori: identification of cytochromes and the effects of oxygen on cytochrome and menaquinone levels. FEMS Microbiol. Lett. 138:59-64.
30. Mukhopadhyay, A. K., J. Y. Jeong, D. Dailidiene, P. S. Hoffman, and D. E. Berg. 2003. The fdxA ferredoxin gene can down regulate frxA nitroreductase gene expression and is essential in many strains of Helicobacter pylori. J. Bacteriol. 185:2927-2935.
31. Olczak, A. A., J. W. Olson, and R. J. Maier. 2002. Oxidative-stress resistance mutants of Helicobacter pylori. J. Bacteriol. 184:3186-3193.
32. Parsonnet, J., G. D. Friedman, D. P. Vandersteen, Y. Chang, J. H. Vogelman, N. Orentreich, and R. K. Sibley. 1991. Helicobacter pylori infection and the risk of gastric carcinoma. N. Engl. J. Med. 325:1127-1131.
33. r thioredoxin reductase. Eur. J. Biochem. 267:6126-6133.
34. Race, P. R., A. L. Lovering, R. M. Green, A. Ossor, S. A. White, P. F. Searle, C. J. Wrighton, and E. I. Hyde. 2005. Structural and mechanistic studies of the Escherichia coli nitroreductase with the antibiotic nitrofurazole. J. Biol. Chem. 280:13256-13264.
35. Sambrook, J., E. F. Fritsch, and T. Maniatis. 1989. Molecular cloning: a laboratory manual, 2nd ed. Cold Spring Harbor Laboratory, Cold Spring Harbor, NY.
36. Schultz, J., F. Milpetz, P. Bork, and C. P. Ponting. 1998. SMART, a simple modular architecture research tool: identification of signaling domains. Proc. Natl. Acad. Sci. USA 95:5857-5864.
37. Sisson, G., A. Goodwin, A. Raudonikiene, N. J. Birks, H. Han, A. Mukhopadhyay, D. E. Berg, and P. S. Hoffman. 2002. Enzymes associated with the reductive activation and action of nitazoxanide, nitrofurans and metronidazole in Helicobacter pylori. Antimicrob. Agents Chemother. 46:2116-2123.
38. + (nitroreductase) gene. J. Bacteriol. 182:5091-5096.
39. Tomb, J. F., O, White, A. R. Kerlavage, R. A. Clayton, G. G. Sutton, R. D. Fleischmann, K. Ketchum, H. Klenk, S. Gill, B. Dougherty, K. Nelson, J. Quackenbush, L. Zhou, E. Kirkness, S. Peterson, B. Loftus, D. Richardson, R. Dodson, H. Khalak, A. Glodek, K. McKenney, L. Fitzegerald, N. Lee, M. Adams, E. Hickey, D. Berg, J. Gocayne, T. Utterback, J. Peterson, J. Kelley, M. Cotton, J. Weidman, C. Fujii, C. Bowman, L. Watthey, E. Wallin, W. Hayes, M. Borodovsky, P. Karp, H. Smith, C. Fraser, and J. Venter. 1997. The complete genome sequence of the gastric pathogen Helicobacter pylori. Nature 388:539-547.
40. Velayndhan, J., and D. J. Kelly. 2002. Analysis of gluconeogenic and anaplerotic enzymes in Campylobacter jejuni: an essential role for phosphoenolpyruvate carboxykinase. Microbiology 148:685-694.
41. Wang, G., P. Alamuri, and R. J. Maier. 2006. The diverse antioxidant systems of Helicobacter pylori. Mol. Microbiol. 61:847-860.
42. Wang, G., and R. J. Maier. 2004. An NADPH quinone reductase of Helicobacter pylori plays an important role in oxidative stress resistance and host colonization. Infect. Immun. 72:1391-1396.
43. Zenno, S., H. Koike, M. Tanokura, and K. Saigo. 1996. Gene cloning, purification, and characterization of NfsB, a minor oxygen-insensitive nitroreductase from Escherichia coli, similar in biochemical properties to Frase I, the major flavin reductase in Vibrio fischeri. J. Biochem. 120:736-744.