Isolation of intact vacuoles from Arabidopsis rosette leaf-derived protoplasts

Nature Protocols

Volumn 2, Issue 2, 2007, Pages 259-262

  Robert, Stéphanie ^a   Zouhar, Jan ^a,b   Carter, Clay ^a,c   Raikhel, Natasha ^a  

^a UNIVERSITY OF CALIFORNIA   (United States)

^b CENTRO NACIONAL DE BIOTECNOLOGÍA   (Spain)

^c UNIVERSITY OF MINNESOTA DULUTH   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ARABIDOPSIS; ARTICLE; CELL COMPARTMENTALIZATION; CELL ISOLATION; CELL VACUOLE; DENSITY GRADIENT; FRACTIONATION; NONHUMAN; OSMOSIS; PHYTOCHEMISTRY; PLANT LEAF; PRIORITY JOURNAL; PROTOPLAST; PURIFICATION; CELL FRACTIONATION; CHEMISTRY; CYTOLOGY; METHODOLOGY;

ARABIDOPSIS;

ARABIDOPSIS; CELL FRACTIONATION; PLANT LEAVES; PROTOPLASTS; VACUOLES;

EID: 34347226331     PISSN: 17542189     EISSN: 17502799     Source Type: Journal    
DOI: 10.1038/nprot.2007.26     Document Type: Article

References (21)

1. Girke, T., Ozkan, M., Carter, D. & Raikhel, N.V. Towards a modeling infrastructure for studying plant cells. Plant Physiol. 132, 410-414 (2003).
2. Millar, A.H., Sweetlove, L.J., Giege, P. & Leaver, C.J. Analysis of the Arabidopsis mitochondrial proteome. Plant Physiol. 127, 1711-1727 (2001).
3. Fitzpatrick, L.M. & Keegstra, K. A method for isolating a high yield of Arabidopsis chloroplasts capable of efficient import of precursor proteins. Plant J. 27, 59-65 (2001).
4. Matile, P. & Wiemken, A. Vacuoles and spherosomes. Methods Enzymol. 31, 572-578 (1974).
5. Cocking, E.C. A method for the isolation of plant protoplasts and vacuoles. Nature 187, 962-963 (1960).
6. Strobel, G.A. & Hess, W.M. Evidence for the presence of the toxin-binding protein on the plasma membrane of sugarcane cells. Proc. Natl. Acad. Sci. USA 71, 1413-1417 (1974).
7. Wagner, G.J. & Siegelman, H.W. Large-scale isolation of intact vacuoles and isolation of chloroplasts from protoplasts of mature plant-tissues. Science 190, 1298-1299 (1975).
8. Boller, T. & Kende, H. Hydrolytic enzymes in the central vacuole of plant-cells. Plant Physiol. 63, 1123-1132 (1979).
9. Sonnewald, U., Brauer, M., von Schaewen, A., Stitt, M. & Willmitzer, L. Transgenic tobacco plants expressing yeast-derived invertase in either the cytosol, vacuole or apoplast: A powerful tool for studying sucrose metabolism and sink/source interactions. Plant J. 1, 95-106 (1991).
10. Gomez, L. & Chrispeels, M.J. Tonoplast and soluble vacuolar proteins are targeted by different mechanisms. Plant Cell 5, 1113-1124 (1993).
11. Leigh, R.A. & Branton, D. Isolation of vacuoles from root storage tissue of Beta vulgaris L. Plant Physiol. 58, 656-662 (1976).
12. Wagner, G.J. Content and vacuole/extravacuole distribution of neutral sugars, free amino acids, and anthocyanin in protoplasts. Plant Physiol. 64, 88-93 (1979).
13. Nishimura, M. & Beevers, H. Hydrolases in vacuoles from castor bean endosperm. Plant Physiol. 62, 44-48 (1978).
14. Granstedt, R.C. & Huffaker, R.C. Identification of the leaf vacuole as a major nitrate storage pool. Plant Physiol. 70, 410-413 (1982).
15. Canovas, F.M. et al. Plant proteome analysis. Proteomics 4, 285-298 (2004).
16. Rossignol, M. et al. Plant proteome analysis: A 2004-2006 update. Proteomics 6, 5529-5548 (2006).
17. Carter, C. et al. The vegetative vacuole proteome of Arabidopsis thaliana reveals predicted and unexpected proteins. Plant Cell 16, 3285-3303 (2004).
18. Shimaoka, T. et al. Isolation of intact vacuoles and proteomic analysis of tonoplast from suspension-cultured cells of Arabidopsis thaliana. Plant Cell Physiol. 45, 672-683 (2004).
19. Rojo, E., Zouhar, J., Carter, C., Kovaleva, V. & Raikhel, N.V. A unique mechanism for protein processing and degradation in Arabidopsis thaliana. Proc. Natl. Acad. Sci. USA 100, 7389-7394 (2003).
20. Dubrovsky, J.G. et al. Neutral red as a probe for confocal laser scanning microscopy studies of plant roots. Ann. Bot. (Lond) 97 1127-1138 (2006).
21. Ahmed, S.U. et al. The plant vacuolar sorting receptor AtELP is involved in transport of NH(2)-terminal propeptide-containing vacuolar proteins in Arabidopsis thaliana. J. Cell. Biol. 149, 1335-1344 (2000).