A method for isolating a high yield of Arabidopsis chloroplasts capable of efficient import of precursor proteins

Plant Journal

Volumn 27, Issue 1, 2001, Pages 59-65

  Fitzpatrick, L M ^a   Keegstra, K ^a  

^a MICHIGAN STATE UNIVERSITY   (United States)

Author keywords

Arabidopsis; ATP; Chloroplast isolation; Protein import

Indexed keywords

AGAR; CHLOROPLAST; CULTURE MEDIUM; PROTEIN BINDING; PROTEIN PRECURSOR; PURIFICATION;

NETWORK PROTOCOLS; PROTEINS; SOILS;

CHLOROPLASTS;

PLANTS (BOTANY);

ARABIDOPSIS; CHLOROPLASTS; ENDOPEPTIDASES; NUCLEOTIDES; PROTEIN TRANSPORT;

ARABIDOPSIS; PISUM SATIVUM;

EID: 0034895894     PISSN: 09607412     EISSN: None     Source Type: Journal    
DOI: 10.1046/j.0960-7412.2001.01061.x     Document Type: Article

References (22)

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3. Kinetic analysis of the transport of thylakoid lumenal proteins in experiments using intact chloroplasts
4. In vitro import of proteins into chloroplasts
5. A mutant deficient in the plastid lipid DGD is defective in protein import into chloroplasts
6. Precursors to two nuclear-encoded chloroplast proteins bind to the outer envelope membrane before being imported into chloroplasts
7. Developmental regulation of the plastid protein import apparatus
8. Energy-dependent uptake of cytoplasmically synthesised polypeptides by chloroplasts
9. Functional analysis of the two Arabidopsis homologues of Toc34, a component of the chloroplast protein import apparatus
10. Adenine nucleotide translocation in spinach chloroplasts
11. An Arabidopsis mutant defective in the plastid general protein import apparatus
12. Protein import and routing systems of chloroplasts
13. T-DNA as an insertional mutagen in Arabidopsis
14. Cleavage of structural proteins during the assembly of the head of bacteriophage T4
15. Efficient in vitro import of a cytosolic heat shock protein into pea chloroplasts
16. ATP is required for the binding of precursor proteins to chloroplasts
17. Protein import in chloroplasts requires a chloroplast ATPase
18. Domains of a transit sequence required for in vivo import in Arabidopsis chloroplasts
19. A chilling-sensitive mutant of Arabidopsis is deficient in chloroplast protein accumulation at low temperature
20. A new chloroplast protein import intermediate reveals distinct translocation machineries in the two envelope membranes: Energetics and mechanistic implications
21. Internal ATP is the only energy requirement for the translocation of precursor proteins across chloroplastic membranes