Enhanced expression, detection and purification of recombinant proteins using RNA stem loop and tandem fusion tags

Applied Microbiology and Biotechnology

Volumn 75, Issue 6, 2007, Pages 1385-1392

  Seleem, Mohamed ^a   Ali, Mohammed ^b   Al Azeem, M W Abd ^c   Boyle, Stephen M ^a   Sriranganathan, Nammalwar ^a  

^a VIRGINIA MARYLAND REGIONAL COLLEGE OF VETERINARY MEDICINE   (United States)

^b Assiut University   (Egypt)

^c South Valley University   (Egypt)

Author keywords

Brucella; Gene expression; His tag; Protein; Purification; RNA stem loop; Vector

Indexed keywords

BIOSYNTHESIS; ESCHERICHIA COLI; GENE EXPRESSION; PURIFICATION; RNA;

PROMOTERS; RECOMBINANT PROTEINS; RNA STEM LOOPS;

PROTEINS;

GREEN FLUORESCENT PROTEIN; HYBRID PROTEIN; RECOMBINANT PROTEIN; RNA;

BACTERIUM; DETECTION METHOD; GENE EXPRESSION; PROTEIN; RECOMBINATION; RNA;

ARTICLE; BRUCELLA; COMPARATIVE STUDY; CONTROLLED STUDY; ESCHERICHIA COLI; EXPRESSION VECTOR; HOST; NONHUMAN; PROTEIN EXPRESSION; PROTEIN PURIFICATION; PROTEIN SYNTHESIS;

BRUCELLA; ESCHERICHIA COLI; HISTIDINE; RECOMBINANT PROTEINS; RNA; SALMONELLA;

BRUCELLA; ESCHERICHIA; ESCHERICHIA COLI; SALMONELLA;

EID: 34250880239     PISSN: 01757598     EISSN: None     Source Type: Journal    
DOI: 10.1007/s00253-007-0970-4     Document Type: Article

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