Improved expression vector for Brucella species

BioTechniques

Volumn 37, Issue 5, 2004, Pages 740-744

  Seleem, Mohamed N ^a   Vemulapalli, Ramesh ^b   Boyle, Stephen M ^a   Schurig, Gerhardt G ^a   Sriranganathan, Nammalwar ^a,c  

^a VIRGINIA POLYTECHNIC INSTITUTE AND STATE UNIVERSITY   (United States)

^b PURDUE UNIVERSITY   (United States)

^c CENTER FOR MOLECULAR MEDICINE AND INFECTIOUS DISEASES   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

GENOMIC DNA; GREEN FLUORESCENT PROTEIN;

ARTICLE; BACTERIAL GENE; BRUCELLA ABORTUS; EXPRESSION VECTOR; GENE EXPRESSION REGULATION; GENE MAPPING; NONHUMAN; NUCLEOTIDE SEQUENCE; PLASMID; POLYMERASE CHAIN REACTION;

BACTERIA (MICROORGANISMS); BRUCELLA; BRUCELLA MELITENSIS BIOVAR ABORTUS; VECTORS;

EID: 8444227485     PISSN: 07366205     EISSN: None     Source Type: Journal    
DOI: 10.2144/04375bm05     Document Type: Article

References (6)

1. Kovach, M.E., R.W. Phillips, P.H. Elzer, R.M. Roop II, and K.M. Peterson. 1994. pBBR1MCS: a broad-host-range cloning vector. BioTechniques 16:800-802.
2. Vemulapalli, R., Y. He, S.M. Boyle, N. Sriranganathan, and G.G. Schurig. 2000. Brucella abortus strain RB51 as a vector for heterologous protein expression and induction of specific Th1 type immune responses. Infect. Immun. 68:3290-3296.
3. Antoine, R. and C. Locht. 1992. Isolation and molecular characterization of a novel broad-host-range plasmid from Bordetella bronchiseptica with sequence similarities to plasmids from Gram-positive organisms. Mol. Microbiol. 6:1785-1799.
4. McQuiston, J.R., G.G. Schurig, N. Sriranganathan, and S.M. Boyle. 1995. Transformation of Brucella species with suicide and broad host-range plasmids. Methods Mol. Biol. 47:143-148.
5. Fernandez-Prada, C.M., E.B. Zelazowska, M. Nikolich, T.L. Hadfield, R.M. Roop II, G.L. Robertson, and D.L. Hoover. 2003. Interactions between Brucella melitensis and human phagocytes: bacterial surface O-polysaccharide inhibits phagocytosis, bacterial killing, and subsequent host cell apoptosis. Infect. Immun. 71:2110-2119.
6. Elzer, P.H., M.E. Kovach, R.W. Phillips, G.T. Robertson, K.M. Peterson, and R.M. Roop II. 1995. In vivo and in vitro stability of the broad-host-range cloning vector pBBR1MCS in six Brucella species. Plasmid 33:51-57.