Positive selection vectors to generate fused genes for the expression of His-Tagged proteins

BioTechniques

Volumn 25, Issue 5, 1998, Pages 898-904

  Van Reeth, T ^a   Dreze P L ^a   Szpirer, J ^a   Szpirer, C ^a   Gabant, P ^a  

^a UNIVERSITÉ LIBRE DE BRUXELLES   (Belgium)

Author keywords

[No Author keywords available]

Indexed keywords

EPITOPE; PROTEIN;

ARTICLE; CLONING VECTOR; ESCHERICHIA COLI; GENE EXPRESSION REGULATION; GENE FUSION; GENE INSERTION; NUCLEOTIDE SEQUENCE;

ESCHERICHIA COLI;

EID: 0031739351     PISSN: 07366205     EISSN: None     Source Type: Journal    
DOI: 10.2144/98255pf01     Document Type: Article

References (12)

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2. Bernard, P. and P. Gabant, inventors. Université Libre de Bruxelles, assignee. Cloning and/or sequencing vector. PCT patent PCT/BE93/00051 and Belgian patent 09200696.
3. Bernard, P., P. Gabant, El.M. Bahassi and M. Couturier. 1994. Positive-selection vectors using the F plasmid ccdB killer gene. Gene 148:71-74.
4. Gabant, P., P.-L. Drèze, T. Van Reeth, J. Szpirer and C. Szpirer. 1997. Bifunctional lacZα-ccdB genes for selective cloning of PCR products. BioTechniques 25:938-941.
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11. Mead, D.A., N.K. Pey, C. Herrnstadt, R.A. Marcil and L.M. Smith. 1991. Universal method for the direct cloning of PCR amplified nucleic acid. Bio/Technology 9:657-663.
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