Evaluation of fatty acid extraction methods for Thraustochytrium sp. ONC-T18

Journal of Agricultural and Food Chemistry

Volumn 55, Issue 12, 2007, Pages 4795-4801

  Burja, Adam M ^a   Armenta, Roberto E ^a,b   Radianingtyas, Helia ^a   Barrow, Colin J ^a  

^a Ocean Nutrition Canada Ltd   (Canada)

^b PEI Food Technology Centre   (Canada)

Author keywords

3 fatty acids; Docosahexaenoic acid; Eicosapentaenoic acid; Extractions; Polyunsaturated fatty acids; Thraustochytrium sp. ONC T18, lipids; Ultrasonics

Indexed keywords

ESTER; FATTY ACID; LIPID; SOLVENT;

ARTICLE; BIOMASS; CELL WALL; CHEMISTRY; FERMENTATION; FUNGUS; GROWTH, DEVELOPMENT AND AGING; ISOLATION AND PURIFICATION; ULTRASTRUCTURE;

BIOMASS; CELL WALL; ESTERS; FATTY ACIDS; FERMENTATION; FUNGI; LIPIDS; SOLVENTS;

THRAUSTOCHYTRIUM SP.;

EID: 34250716332     PISSN: 00218561     EISSN: None     Source Type: Journal    
DOI: 10.1021/jf070412s     Document Type: Article

References (29)

1. Covington, M. B. Omega-3 fatty acids. Am. Fam. Phys. 2004, 70, 133-140.
2. Nettleton, J. A. Omega-3 fatty acids: comparison of plant and seafood sources in human nutrition. J. Am. Diet. Assoc. 1991, 91, 331-337.
3. Napier, J. A. Plumbing the depths of PUFA biosynthesis: A novel polyketide synthase-like pathway from marine organisms. Trends Plant Sci. 2002, 7, 51-54.
4. Aki, T.; Hachida, K.; Yoshinaga, M.; Katai, Y.; Yamasaki, T.; Kawamoto, S.; Kakizono, T.; Maoka, T.; Shigeta, S.; Suzuki, O.; Ono, K. Thraustochytrid as a potential source of carotenoids. J. Am. Oil Chem. Soc. 2003, 80, 789-794.
5. Lewis, T. E.; Nichols, P. D.; McMeekin, T. A. The biotechnological potential of thraustochytrids. Mar. Biotechnol. 1999, 1, 580-587.
6. Burja, A. M.; Radianingtyas, H.; Windust, A.; Barrow, C. J. Isolation and characterization of polyunsaturated fatty acid producing Thraustochytrium species: Screening of strains and optimization of omega-3 production. Appl. Microbiol. Biotechnol. 2006, 72, 1161-1169.
7. Cartens, M.; Grima, E. M.; Medina, A. R.; Gimenez, A. C.; Gonzalez, J. I. Eicosapentaenoic acid (20:5n-3) from the marine microalga Phaeodactylum tricornutum. J. Am. Oil Chem. Soc. 1996, 73, 1025-1031.
8. Grima, E. M.; Medina, A. R.; Gimenez, A. G.; Perez, J. A. S.; Camacho, F. G.; Sanchez, J. L. G. Comparison between extraction of lipids and fatty acids from microalgal biomass. J. Am. Oil Chem. Soc. 1994, 71, 955-959.
9. Lewis, T.; Nichols, P. D.; McMeekin, T. A. Evaluation of extraction methods for recovery of fatty acids from lipid-producing microheterotrophs. J. Microbiol. Methods 2000, 43, 107-116.
10. Nishihara, M.; Koga, Y. Extraction and composition of polar lipids from the Archaebacterium, Methanobacterium thermoautotrophicum: Effective extraction of tetraether lipids by an acidified solvent. J. Biochem. 1987, 101, 997-1005.
11. Pinkart, H. C.; Devereux, R.; Chapman, P. J. Rapid separation of microbial lipids using solid phase extraction columns. J. Microbiol. Methods 1998, 34, 9-15.
12. Bligh, E. G.; Dyer, W. J. A Rapid method of total lipid extraction and purification. Can. J. Biochem. Phys. 1959, 37, 911-917.
13. White, D. C.; Davis, W. M.; Nickels, J. S.; King, J. D.; Bobbie, R. J. Determination of the sedimentary microbial biomass by extractable lipid phosphate. Oecologia 1979, 40, 51-62.
14. Dunstan, G. A.; Volkman, J. K.; Barrett, S. M. The effect of lyophilization on the solvent-extraction of lipid classes, fatty-acids and sterols from the oyster Crassostrea gigas. Lipids 1993, 28, 937-944.
15. Dunstan, G. A.; Volkman, J. K.; Jeffrey, S. W.; Barrett, S. M. Biochemical-composition of microalgae from the green algal classes Chlorophyceae and Prasinophyceae. 2. Lipid classes and fatty-acids. J. Exp. Mar. Biol. Ecol. 1992, 161, 115-134.
16. Kehrmeyer, S. R.; Applegate, B. M.; Pinkart, H. C.; Hedrick, D. B.; White, D. C.; Sayler, G. S. Combined lipid/DNA extraction method for environmental samples. J. Microbiol. Methods 1996, 25, 153-163.
17. Fernandez-Reiriz, M. J.; Labarta, U. Lipid classes and fatty acid composition of rotifers (Brachionus plicatilis) fed two algal diets. Hydrobiologia 1996, 330, 73-79.
18. Funteu, F.; Guet, C.; Wu, B. Z.; Tremolieres, A. Effects of environmental factors on the lipid metabolism in Spirulina platensis. Plant Phys. Biochem. 1997, 35, 63-71.
19. Gonzalez, M. J. I.; Medina, A. R.; Grima, E. M.; Gimenez, A. G.; Cartens, M.; Cerdan, L. E. Optimization of fatty acid extraction from Phaeodactylum tricornutum UTEX 640 biomass. J. Am. Oil Chem. Soc. 1998, 75, 1735-1740.
20. Guil-Guerrero, J. L.; Belarbi, E. H.; Rebolloso-Fuentes, M. M. Eicosapentaenoic and arachidonic acids purification from the red microalga Porphyridium cruentum. Bioseparation 2000, 9, 299-306.
21. Guckert, J. B.; White, D. C. Evaluation of a hexane isopropanol lipid solvent system for analysis of bacterial phospholipids and application to chloroform-soluble nuclepore (polycarbonate) membranes with retained bacteria. J. Microbiol. Methods 1988, 8, 131-137.
22. Guckert, J. B.; Cooksey, K. E.; Jackson, L. L. Lipid solvent systems are not equivalent for analysis of lipid classes in the microeukaryotic green alga, Chlorella. J. Microbiol. Methods 1988, 8, 139-149.
23. DeLong, E. F.; Yayanos, A. A. Adaptation of the membrane lipids of a deep-sea bacterium to changes in hydrostatic pressure. Science 1985, 228, 1101-1103.
24. Kenyon, C. N. Fatty-acid composition of unicellular strains of blue-green algae. J. Bacteriol. 1972, 109, 827-834.
25. Bowles, R. D.; Hunt, A. E.; Bremer, G. B.; Duchars, M. G.; Eaton, R. A. Long-chain n-3 polyunsaturated fatty acid production by members of the marine protistan group the thraustochytrids: screening of isolates and optimisation of docosahexaenoic acid production. J. Biotechnol. 1999, 70, 193-202.
26. AOAC Official Method 970.51. Fats (animal) in vegetable fats and oils (determination of cholesterol). In Official Methods of Analysis of AOAC International, 17th ed.; Gaithersburg, MD, 2002; Vol. II, pp 47-49.
27. Mason, T. J.; Lorimer, J. P. Introduction to applied ultrasonics. In Applied Sonochemistry; Wiley-VCH Verlag GmbH: Weinheim, 2002; pp 1-24.
28. Sijtsma, L.; de Swaaf, M. E. Biotechnological production and applications of the Ω-3 polyunsaturated fatty acid docosahexaenoic acid. Appl. Microbiol. Biotechnol. 2004, 64, 146-153.
29. Bockisch, M. Importance of fats, and animal fats and oils. In Fats and Oils Handbook; AOCS Press: Chicago, IL, 1993; pp 1-52 and 121-173.