A 2′-methyl or 2′-methylene group at G+1 in precursor tRNA interferes with Mg2+ binding at the enzyme-substrate interface in E-S complexes of E. coli RNase P

Biological Chemistry

Volumn 388, Issue 7, 2007, Pages 717-726

  Cuzic, Simona ^a   Hartmann, Roland K ^a  

^a PHILIPPS UNIVERSITY MARBURG   (Germany)

Author keywords

2 OCH3 substitutions at G+1 in the substrate; Escherichia coli RNase P; LNA (locked nucleic acid); Precursor tRNA processing

Indexed keywords

CARBENE; LOCKED NUCLEIC ACID; MAGNESIUM; MANGANESE; METAL ION; METHYL GROUP; RIBONUCLEASE P; RNA PRECURSOR; TRANSFER RNA; ADENINE; DIVALENT CATION; GUANINE; METHANE;

ARTICLE; BINDING SITE; BIOCHEMISTRY; CHEMICAL BINDING; COMPLEX FORMATION; CONTROLLED STUDY; ENZYME BINDING; ENZYME DEGRADATION; ENZYME SUBSTRATE; ESCHERICHIA COLI; GENE DISRUPTION; NONHUMAN; PRIORITY JOURNAL; RNA PROCESSING; RNA STRUCTURE; CHEMICAL STRUCTURE; CHEMISTRY; CONFORMATION; ENZYME SPECIFICITY; ENZYMOLOGY; GENETICS; METABOLISM; METHYLATION; MOLECULAR GENETICS; NUCLEOTIDE SEQUENCE; PROTEIN BINDING; PROTEIN TERTIARY STRUCTURE; RNA INTERFERENCE;

ESCHERICHIA COLI;

ADENINE; BASE SEQUENCE; CATIONS, DIVALENT; ESCHERICHIA COLI; GUANINE; MAGNESIUM; METHANE; METHYLATION; MODELS, MOLECULAR; MOLECULAR SEQUENCE DATA; NUCLEIC ACID CONFORMATION; PROTEIN BINDING; PROTEIN STRUCTURE, TERTIARY; RIBONUCLEASE P; RNA INTERFERENCE; RNA PRECURSORS; RNA, TRANSFER; SUBSTRATE SPECIFICITY;

EID: 34250621718     PISSN: 14316730     EISSN: 14374315     Source Type: Journal    
DOI: 10.1515/BC.2007.095     Document Type: Article

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