Differential role of the intermolecular base-pairs G292-C75 and G293-C74 in the reaction catalyzed by Escherichia coli RNase P RNA

Journal of Molecular Biology

Volumn 299, Issue 4, 2000, Pages 941-951

  Busch, Silke ^a   Kirsebom, Leif A ^b   Notbohm, Holger ^a   Hartmann, Roland K ^a  

^a UNIVERSITY OF LÜBECK   (Germany)

^b UPPSALA UNIVERSITY   (Sweden)

Author keywords

Point mutations; RCCA RNase P RNA interaction; Ribonuclease P; Single turnover kinetics; tRNA binding

Indexed keywords

BACTERIAL RNA; CYTOSINE; FLUORESCEIN; GUANINE; RIBONUCLEASE P; TRANSFER RNA;

ARTICLE; BASE PAIRING; CATALYSIS; CHEMICAL REACTION KINETICS; CIRCULAR DICHROISM; CONTROLLED STUDY; DEPHOSPHORYLATION; ESCHERICHIA COLI; FLUORESCENCE; NONHUMAN; NUCLEIC ACID BASE SUBSTITUTION; POINT MUTATION; PRIORITY JOURNAL; THERMODYNAMICS;

ESCHERICHIA COLI;

EID: 0034674157     PISSN: 00222836     EISSN: None     Source Type: Journal    
DOI: 10.1006/jmbi.2000.3789     Document Type: Article

References (34)

1. Ribonuclease P
2. RNase P from a photosynthetic organelle contains an RNA homologous to the cyanobacterial counterpart
3. A kinetic mechanism for cleavage of precursor tRNA(Asp) catalyzed by the RNA component of Bacillus subtilis ribonuclease P
4. Nuclear retention of RNA as a mechanism for localization
5. RNase P RNA structure and cleavage reflect the primary structure of tRNA genes
6. 3'-phosphatase activity in T4 polynucleotide kinase
7. Lead-ion induced cleavage of RNase P RNA
8. Ribonuclease P RNA: Models of the 15/16 bulge from Escherichia coli and the P15 stem loop of Bacillus subtilis
9. Hydrogen bonding and biological specificity analysed by protein engineering
10. Improved free-energy parameters for predictions of RNA duplex stability
11. Differences in the interaction of Escherichia coli RNase P RNA with tRNAs containing a short or a long extra arm
12. The RNA moiety of ribonuclease P is the catalytic subunit of the enzyme
13. Gel retardation analysis of E. coli M1 RNA-tRNA complexes
14. Kinetics and thermodynamics of the RNase P RNA cleavage reaction: Analysis of tRNA 3'-end variants
15. Rp-phosphorothioate modifications in RNase P RNA that interfere with tRNA binding
16. 2 groups of E. coli RNase P RNA involved in intramolecular tertiary contacts and direct interactions with tRNA
17. RNase P RNA from Prochlorococcus marinus: Contribution of substrate domains to recognition by a cyanobacterial ribozyme
18. RNase P and its substrate
19. Base pairing between Escherichia coli RNase P RNA and its substrate
20. Protection from chemical modification of nucleotides in complexes of M1 RNA, the catalytic subunit of RNase P from E. coli, and tRNA precursors
21. Residues in Escherichia coli RNase P RNA important for cleavage site selection and divalent metal ion binding
22. The P15-loop of Escherichia coli RNase P RNA is an autonomous divalent metal ion binding domain
23. Formation of SRP-like particle induces a conformational change in E. coli 4.5 S RNA
24. The cleavage step of ribonuclease P catalysis is determined by ribozyme-substrate interactions both distal and proximal to the cleavage site
25. 2+ ions by ribonuclease P
26. Intermediates and kinetic traps in the folding of a large ribozyme revealed by circular dichroism and UV absorbance spectroscopies and catalytic activity
27. Substrate binding and catalysis by ribonuclease P from cyanobacteria and Escherichia coli are affected differently by the 3' terminal CCA in tRNA precursors
28. Transient conformational states of aminoacyl-tRNA during ribosome binding catalyzed by elongation factor Tu
29. Energetics and cooperativity of tertiary hydrogen bonds in RNA structure
30. Phylogenetic comparative mutational analysis of the base-pairing between RNase P RNA and its substrate
31. The RNase P RNA from cyanobacteria: Short tandemly repeated repetitive (STRR) sequences are present within the RNase P RNA gene in heterocyst-forming cyanobacteria
32. Protein-RNA interactions in the RNase P holoenzyme from Escherichia coli
33. 2+-ribozyme by a single Rp-phosphorothioate modification in the precursor tRNA at the RNase P cleavage site
34. Role of metal ions in the hydrolysis reaction catalyzed by RNase P RNA from Bacillus subtilis