Osteogenic differentiation of adipose derived stem cells promoted by overexpression of osterix

Molecular and Cellular Biochemistry

Volumn 301, Issue 1-2, 2007, Pages 83-92

  Wu, Ling ^a   Wu, Yao ^a   Lin, Yunfeng ^a   Jing, Wei ^a   Nie, Xin ^a   Qiao, Ju ^a   Liu, Lei ^a   Tang, Wei ^a   Tian, Weidong ^a  

^a SICHUAN UNIVERSITY   (China)

Author keywords

Adipose derived stem cells; Osteoblasts; Osteogenic differentiation; Osterix; Overexpression

Indexed keywords

ALKALINE PHOSPHATASE; BONE SIALOPROTEIN; COLLAGEN TYPE 1; MATRIX PROTEIN; OSTEOCALCIN; OSTEOPONTIN; PROTEIN OSTERIX; TRANSCRIPTION FACTOR; UNCLASSIFIED DRUG;

ADIPOSE TISSUE CELL; ANIMAL CELL; ANIMAL TISSUE; ARTICLE; CELL DIFFERENTIATION; CELL PROLIFERATION; CELLULAR DISTRIBUTION; CONTROLLED STUDY; DNA MODIFICATION; ENZYME ACTIVITY; GENE CONSTRUCT; GENE OVEREXPRESSION; GENETIC TRANSFECTION; HYPOTHESIS; IMMUNOHISTOCHEMISTRY; IN VITRO STUDY; MOUSE; NONHUMAN; NUCLEOTIDE SEQUENCE; OSTEOBLAST; PLASMID; PROTEIN LOCALIZATION; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; STEM CELL; TISSUE ENGINEERING;

ADIPOSE TISSUE; ANIMALS; BONE AND BONES; CALCIFICATION, PHYSIOLOGIC; CELL DIFFERENTIATION; CELL PROLIFERATION; CELLS, CULTURED; CULTURE MEDIA; GENE EXPRESSION REGULATION; MICE; MICE, INBRED BALB C; OSTEOGENESIS; STEM CELLS; TRANSCRIPTION FACTORS;

MURINAE;

EID: 34250209505     PISSN: 03008177     EISSN: 15734919     Source Type: Journal    
DOI: 10.1007/s11010-006-9399-9     Document Type: Article

References (45)

1. Grove JE, Bruscia E, Krause DS (2004) Plasticity of bone marrow-derived stem cells. Stem Cells 22:487-500
2. Pomerantz J, Blau HM (2004) Nuclear reprogramming: A key to stem cell function in regenerative medicine. Nat Cell Biol 6:810-816
3. Kofron MD, Laurencin CT (2006) Bone tissue engineering by gene delivery. Adv Drug Deliv Rev 58:555-576
4. Zuk PA, Zhu M, Mizuno H, Huang J, Futrell JW, Katz AJ, Benhaim P, Lorenz HP, Hedrick MH (2001) Multilineage cells from human adipose tissue: implications for cell-based therapies. Tissue Eng 7:211-228
5. Lin Y, Liu L, Li Z, Qiao J, Wu L, Tang W, Zheng X, Chen X, Yan Z, Tian W (2006) Pluripotency potential of human adipose-derived stem cells marked with exogenous green fluorescent protein. Mol Cell Biochem 291:1-10
6. Zuk PA, Zhu M, Ashjian P, De Ugarte DA, Huang JI, Mizuno H, Alfonso ZC, Fraser JK, Benhaim P, Hedrick MH (2002) Human adipose tissue is a source of multipotent stem cells. Mol Cell Biol 13:4279-4295
7. Reyes M, Lund T, Lenvik T, Aguiar D, Koodie L, Verfaillie CM (2001) Purification and ex vivo expansion of postnatal human marrow mesodermal progenitor cells. Blood 98:2615-2625
8. Jaiswal N, Haynesworth SE, Caplan AI, Bruder SP (1997) Osteogenic differentiation of purified, culture expanded human mesenchymal stem cells in vitro. J Cell Biochem 64:295-312
9. Majumdar MK, Thiede MA, Haynesworth SE, Bruder SP, Gerson SL (2000) Human marrow-derived mesenchymal stem cells (MSCs) express hematopoietic cytokines and support long-term hematopoiesis when differentiated toward stromal and osteogenic lineages. J Hematother Stem Cell Res 9:841-848
10. Zhang X, Yang M, Lin L, Chen P, Ma KT, Zhou CY, Ao YF (2006) Runx2 overexpression enhances osteoblastic differentiation and mineralization in adipose-derived stem cells in vitro and in vivo. Calcif Tissue Int 79:169-78
11. Wan DC, Shi YY, Nacamuli RP, Quarto N, Lyons KM, Longaker MT (2006) Osteogenic differentiation of mouse adipose-derived adult stromal cells requires retinoic acid and bone morphogenetic protein receptor type IB signaling. Proc Natl Acad Sci USA 103:12335-12340
12. Quarto N, Longaker MT (2006) FGF-2 inhibits osteogenesis in mouse adipose tissue-derived stromal cells and sustains their proliferative and osteogenic potential state. Tissue Eng 12:1405-1418
13. Hattori H, Sato M, Masuoka K, Ishihara M, Kikuchi T, Matsui T, Takase B, Ishizuka T, Kikuchi M, Fujikawa K, Ishihara M (2004) Osteogenic potential of human adipose tissue-derived stromal cells as an alternative stem cell source. Cells Tissues Organs 178:2-12
14. Nakashima K, Zhou X, Kunkel G, Zhang Z, Deng JM, Behringer RR, de Crombrugghe B (2002) The novel zinc finger-containing transcription factor osterix is required for osteoblast differentiation and bone formation. Cell 108:17-29
15. Philipsen S, Suske G (1999) A tale of three fingers: The family of mammalian Sp/XKLF transcription factors. Nucleic Acids Res 27:2991-3000
16. Milona MA, Gough JE, Edgar AJ (2003) Expression of alternatively spliced isoforms of human Sp7 in osteoblast-like cells. BMC Genomics 4:43-53
17. Yagi K, Tsuji K, Nifuji A, Shinomiya K, Nakashima K, DeCrombrugghe B, Noda M (2003) Bone morphogenetic protein-2 enhances osterix gene expression in chondrocytes. J Cell Biochem 88:1077-1083
18. Cao Y, Zhou Z, de Crombrugghe B, Nakashima K, Guan H, Duan X, Jia SF, Kleinerman ES (2005) Osterix, a transcription factor for osteoblast differentiation, mediates antitumor activity in murine osteosarcoma. Cancer Res 65:1124-1128
19. Tai G, Christodoulou I, Bishop AE, Polak JM (2005) Use of green fluorescent fusion protein to track activation of the transcription factor osterix during early osteoblast differentiation. Biochem Biophys Res Commun 333:1116-1122
20. Tu Q, Valverde P, Chen J (2006) Osterix enhances proliferation and osteogenic potential of bone marrow stromal cells. Biochem Biophys Res Commun 341:1257-1265
21. Lin Y, Tian W, Chen X, Yan Z, Li Z, Qiao J, Liu L, Tang W, Zheng X (2005) Expression of exogenous or endogenous green fluorescent protein in adipose tissue-derived stromal cells during chondrogenic differentiation. Mol Cell Biochem 277:181-190
22. Gao Y, Jheon A, Nourkeyhani H, Kobayashi H, Ganss B (2004) Molecular cloning, structure, expression, and chromosomal localization of the human Osterix (SP7) gene. Gene 341:101-110
23. Lin Y, Chen X, Yan Z, Liu L, Tang W, Zheng X, Li Z, Qiao J, Li S, Tian W (2006) Multilineage differentiation of adipose-derived stromal cells from GFP transgenic mice. Mol Cell Biochem 285:69-78
24. Mosmann T (1983) Rapid colorimetric assay for cellular growth and survival: Application to proliferation and cytotoxicity assays. J Immunol Methods 65:55-63
25. Johnson RL, Tabin CJ (1997) Molecular models for vertebrate limb development. Cell 90:979-990
26. Olsen BR, Reginato AM, Wang W (2000) Bone development. Annu Rev Cell Dev Biol 16:191-220
27. Harada S, Rodan GA (2003) Control of osteoblast function and regulation of bone mass. Nature 423:349-355
28. Ganss B, Kim RH, Sodek J (1999) Bone sialoprotein. Crit Rev Oral Biol Med 10:79-98
29. Viereck V, Siggelkow H, Tauber S, Raddatz D, Schutze N, Hufner M (2002) Differential regulation of Cbfa1/Runx2 and osteocalcin gene expression by vitamin-D3, dexamethasone, and local growth factors in primary human osteoblasts. J Cell Biochem 86:348-356
30. Standal T, Borset M, Sundan A (2004) Role of osteopontin in adhesion, migration, cell survival and bone remodeling. Exp Oncol 26:179-184
31. Beck GR Jr, Sullivan EC, Moran E, Zerler B (1998) Relationship between alkaline phosphatase levels, osteopontin expression, and mineralization in differentiating MC3T3-E1 osteoblasts. J Cell Biochem 68:269-280
32. Tai G, Polak JM, Bishop AE, Christodoulou I, Buttery LD (2004) Differentiation of osteoblasts from murine embryonic stem cells by overexpression of the transcriptional factor osterix. Tissue Eng 10:1456-1466
33. Kim YJ, Kim HN, Park EK, Lee BH, Ryoo HM, Kim SY, Kim IS, Stein JL, Lian JB, Stein GS, van Wijnen AJ, Choi JY (2006) The bone-related Zn finger transcription factor Osterix promotes proliferation of mesenchymal cells. Gene 366:145-151
34. Huang W, Rudkin GH, Carlsen B, Ishida K, Ghasri P, Anvar B, Yamaguchi DT, Miller TA (2002) Overexpression of BMP-2 modulates morphology, growth, and gene expression in osteoblastic cells. Exp Cell Res 274:226-234
35. Cho HH, Park HT, Kim YJ, Bae YC, Suh KT, Jung JS (2005) Induction of osteogenic differentiation of human mesenchymal stem cells by histone deacetylase inhibitors. J Cell Biochem 96:533-542
36. Igarashi M, Kamiya N, Hasegawa M, Kasuya T, Takahashi T, Takagi M (2004) Inductive effects of dexamethasone on the gene expression of Cbfa1, Osterix and bone matrix proteins during differentiation of cultured primary rat osteoblasts. J Mol Histol 35:3-10
37. Lee RH, Kim B, Choi I, Kim H, Choi HS, Suh K, Bae YC, Jung JS (2004) Characterization and expression analysis of mesenchymal stem cells from human bone marrow and adipose tissue. Cell Physiol Biochem 14:311-324
38. Bouwman P, Philipsen S (2002) Regulation of the activity of Sp1-related transcription factors. Mol Cell Endocrinol 195:27-38
39. Ganss B, Jheon A (2004) Zinc finger transcription factors in skeletal development. Crit Rev Oral Biol Med 15:282-297
40. Opitz O, Rustgi A (2000) Interaction between Sp1 and cell cycle regulatory proteins is important in transactivation of a differentiation-related gene. Cancer Res, 60:2825-2830
41. Aslam F, Palumbo L, Augenlicht LH, Velcich A (2001) The Sp family of transcription factors in the regulation of the human and mouse MUC2 gene promoters. Cancer Res 61:570-576
42. Kaczynski J, Cook T, Urrutia R (2003) Sp1- and Krüppel-like transcription factors. Genome Biol 4:206-213
43. Wu D, Razzano P, Grande DA (2003) Gene therapy and tissue engineering in repair of the musculoskeletal system. J Cell Biochem 88:467-481
44. Gazit D, Turgeman G, Kelley P, Wang E, Jalenak M, Zilberman Y, Moutsatsos I (1999) Engineered pluripotent mesenchymal cells integrate and differentiate in regenerating bone: A novel cell-mediated gene therapy. J Gene Med 1:121-133
45. Pola E, Gao W, Zhou Y, Pola R, Lattanzi W, Sfeir C, Gambotto A, Robbins PD (2004) Efficient bone formation by gene transfer of human LIM mineralization protein-3. Gene Ther 11:683-693