Use of green fluorescent fusion protein to track activation of the transcription factor osterix during early osteoblast differentiation

Biochemical and Biophysical Research Communications

Volumn 333, Issue 4, 2005, Pages 1116-1122

  Tai, Guangping ^a,b   Christodoulou, Ioannis ^a,b   Bishop, Anne E ^a   Polak, Julia M ^a  

^a IMPERIAL COLLEGE LONDON   (United Kingdom)

^b UNIVERSITY OF EDINBURGH   (United Kingdom)

Author keywords

Differentiation; Embryonic stem cells; Osteoblasts; Osterix

Indexed keywords

GREEN FLUORESCENT PROTEIN; HYBRID PROTEIN; TRANSCRIPTION FACTOR; TRANSCRIPTION FACTOR OSTERIX; UNCLASSIFIED DRUG;

ANIMAL CELL; ANIMAL CELL CULTURE; ARTICLE; BONE DEVELOPMENT; CELL DIFFERENTIATION; CELL LINEAGE; CELLULAR DISTRIBUTION; CONTROLLED STUDY; CYTOPLASM; EMBRYO; EMBRYONIC STEM CELL; HUMAN; HUMAN CELL; HUMAN CELL CULTURE; MESENCHYMAL STEM CELL; MESENCHYME CELL; NONHUMAN; OSTEOBLAST; PRIORITY JOURNAL; PROADIPOCYTE; PROTEIN ANALYSIS; PROTEIN FUNCTION; PROTEIN LOCALIZATION; RODENT; SYNOVIOCYTE;

3T3-L1 CELLS; ANIMALS; CELL DIFFERENTIATION; CELL LINE; GREEN FLUORESCENT PROTEINS; HUMANS; MESENCHYMAL STEM CELLS; MICE; MICROSCOPY, FLUORESCENCE; NIH 3T3 CELLS; OSTEOBLASTS; RECOMBINANT FUSION PROTEINS; TRANS-ACTIVATION (GENETICS); TRANSCRIPTION FACTORS;

MURINAE;

EID: 21344440967     PISSN: 0006291X     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.bbrc.2005.05.195     Document Type: Article

References (16)

1. P. Ducy, R. Zhang, V. Geoffroy, A.L. Ridall, and G. Karsenty Osf2/Cbfa1: a transcriptional activator of osteoblast differentiation Cell 89 1997 747 754
2. X. Yang, and G. Karsenty Transcription factors in bone: developmental and pathological aspects Trends Mol. Med. 8 2002 340 345
3. J.B. Lian, A. Javed, S.K. Zaidi, C. Lengner, M. Montecino, A.J. van Wijnen, J.L. Stein, and G.S. Stein Regulatory controls for osteoblast growth and differentiation: role of Runx/Cbfa/AML factors Crit. Rev. Eukaryot. Gene Expr. 14 2004 1 41
4. K. Nakashima, X. Zhou, G. Kunkel, Z. Zhang, J.M. Deng, R.R. Behringer, and C.B. de The novel zinc finger-containing transcription factor osterix is required for osteoblast differentiation and bone formation Cell 108 2002 17 29
5. K. Nakashima, and C.B. de Transcriptional mechanisms in osteoblast differentiation and bone formation Trends Genet. 19 2003 458 466
6. J.B. Lian, and G.S. Stein Runx2/Cbfa1: a multifunctional regulator of bone formation Curr. Pharm. Des. 9 2003 2677 2685
7. D.S. de Jong, B.L. Vaes, K.J. Dechering, A. Feijen, J.M. Hendriks, R. Wehrens, C.L. Mummery, E.J. van Zoelen, W. Olijve, and W.T. Steegenga Identification of novel regulators associated with early-phase osteoblast differentiation J. Bone Miner. Res. 19 2004 947 958
8. C.B. de, V. Lefebvre, and K. Nakashima Regulatory mechanisms in the pathways of cartilage and bone formation Curr. Opin. Cell Biol. 13 2001 721 727
9. I.D. Xynos, A.J. Edgar, L.D. Buttery, L.L. Hench, and J.M. Polak Ionic products of bioactive glass dissolution increase proliferation of human osteoblasts and induce insulin-like growth factor II mRNA expression and protein synthesis Biochem. Biophys. Res. Commun. 276 2000 461 465
10. L.D. Buttery, S. Bourne, J.D. Xynos, H. Wood, F.J. Hughes, S.P. Hughes, V. Episkopou, and J.M. Polak Differentiation of osteoblasts and in vitro bone formation from murine embryonic stem cells Tissue Eng. 7 2001 89 99
11. G. Tai, J.M. Polak, A.E. Bishop, I. Christodoulou, and L.D. Buttery Differentiation of osteoblasts from murine embryonic stem cells by overexpression of the transcriptional factor osterix Tissue Eng. 10 2004 1456 1466
12. R.C. Bielby, A.R. Boccaccini, J.M. Polak, and L.D. Buttery In vitro differentiation and in vivo mineralization of osteogenic cells derived from human embryonic stem cells Tissue Eng. 10 2004 1518 1525
13. K. Sakai, L. Hiripi, V. Glumoff, O. Brandau, R. Eerola, E. Vuorio, Z. Bosze, R. Fassler, and A. Aszodi Stage- and tissue-specific expression of a Col2a1-Cre fusion gene in transgenic mice Matrix Biol. 19 2001 761 767
14. J.E. Kim, K. Nakashima, and C.B. de Transgenic mice expressing a ligand-inducible cre recombinase in osteoblasts and odontoblasts: a new tool to examine physiology and disease of postnatal bone and tooth Am. J. Pathol. 165 2004 1875 1882
15. J.E. Aubin Bone stem cells J. Cell. Biochem. Suppl. 30-31 1998 73 82
16. J.Y. Choi, J. Pratap, A. Javed, S.K. Zaidi, L. Xing, E. Balint, S. Dalamangas, B. Boyce, A.J. van Wijnen, J.B. Lian, J.L. Stein, S.N. Jones, and G.S. Stein Subnuclear targeting of Runx/Cbfa/AML factors is essential for tissue-specific differentiation during embryonic development Proc. Natl. Acad. Sci. USA 98 2001 8650 8655