Hemi-nested touchdown PCR combined with primer-template mismatch PCR for rapid isolation and sequencing of low molecular weight glutenin subunit gene family from a hexaploid wheat BAC library

BMC Genetics

Volumn 8, Issue , 2007, Pages

  Huang, Xiu Qiang ^a   Cloutier, Sylvie ^a  

^a AGRICULTURE AND AGRI FOOD CANADA   (Canada)

Author keywords

[No Author keywords available]

Indexed keywords

ADENINE; ALCOHOL; GENOMIC DNA; GLUTENIN; GUANINE; PLANT DNA; THYMINE; GLUTEN; PRIMER DNA;

5' UNTRANSLATED REGION; ARTICLE; BACTERIAL ARTIFICIAL CHROMOSOME; BIOFILTER; CONSENSUS SEQUENCE; CONTROLLED STUDY; GENE AMPLIFICATION; GENE ISOLATION; GENETIC CODE; HEMINESTED TOUCHDOWN POLYMERASE CHAIN REACTION; HEXAPLOIDY; INTERMETHOD COMPARISON; MOLECULAR CLONING; MULTIGENE FAMILY; NONHUMAN; NUCLEOTIDE SEQUENCE; PLANT GENETICS; POLYMERASE CHAIN REACTION; PRIMER TEMPLATE MISMATCH POLYMERASE CHAIN REACTION; PURIFICATION; SEQUENCE ANALYSIS; SEQUENCE HOMOLOGY; TEMPERATURE SENSITIVITY; WHEAT; GENE; GENE LIBRARY; GENETICS; METHODOLOGY; MOLECULAR WEIGHT; POLYPLOIDY; SEQUENCE ALIGNMENT;

BACTERIA (MICROORGANISMS); TRITICUM AESTIVUM;

BASE SEQUENCE; CHROMOSOMES, ARTIFICIAL, BACTERIAL; CLONING, MOLECULAR; DNA PRIMERS; GENE LIBRARY; GENES, PLANT; GLUTEN; MOLECULAR WEIGHT; POLYMERASE CHAIN REACTION; POLYPLOIDY; SEQUENCE ALIGNMENT; TRITICUM;

EID: 34250175858     PISSN: None     EISSN: 14712156     Source Type: Journal    
DOI: 10.1186/1471-2156-8-18     Document Type: Article

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