메뉴 건너뛰기




Volumn 2, Issue 1, 2007, Pages 187-190

Live-cell assay for detection of apoptosis by dual-laser flow cytometry using Hoechst 33342 and 7-amino-actinomycin D

Author keywords

[No Author keywords available]

Indexed keywords

7 AMINODACTINOMYCIN; FLUORESCEIN ISOTHIOCYANATE; FLUORESCENT DYE; HOE 33342; MEMBRANE ANTIGEN; MONOCLONAL ANTIBODY; 7-AMINOACTINOMYCIN D; BENZIMIDAZOLE DERIVATIVE; DACTINOMYCIN; DIAGNOSTIC AGENT; DRUG DERIVATIVE; UNCLASSIFIED DRUG;

EID: 34249999528     PISSN: 17542189     EISSN: 17502799     Source Type: Journal    
DOI: 10.1038/nprot.2006.458     Document Type: Article
Times cited : (86)

References (19)
  • 2
    • 0026492817 scopus 로고
    • Features of apoptotic cells measured by flow cytometry
    • Darzynkiewicz, Z. et al. Features of apoptotic cells measured by flow cytometry. Cytometry 13, 795-808 (1992).
    • (1992) Cytometry , vol.13 , pp. 795-808
    • Darzynkiewicz, Z.1
  • 3
    • 0027979892 scopus 로고
    • Sensitive method for measuring apoptosis and cell surface phenotype in human thymocytes by flow cytometry
    • Schmid, I., Uittenbogaart, C.H. & Giorgi, J.V. Sensitive method for measuring apoptosis and cell surface phenotype in human thymocytes by flow cytometry. Cytometry 15, 12-20 (1994).
    • (1994) Cytometry , vol.15 , pp. 12-20
    • Schmid, I.1    Uittenbogaart, C.H.2    Giorgi, J.V.3
  • 4
    • 0026543962 scopus 로고
    • Analysis and discrimination of necrosis and apoptosis (programmed cell death) by multiparameter flow cytometry
    • Dive, C. et al. Analysis and discrimination of necrosis and apoptosis (programmed cell death) by multiparameter flow cytometry. Biochim. Biophys. Acta 1133, 275-285 (1992).
    • (1992) Biochim. Biophys. Acta , vol.1133 , pp. 275-285
    • Dive, C.1
  • 5
    • 0026655823 scopus 로고
    • Apoptosis in interleukin-3-dependent haemopoietic cells: Quantification by two flow cytometric methods
    • Ormerod, M.G., Collins, M.K.L., Rodriguez-Tarduchy, G. & Robertson, D. Apoptosis in interleukin-3-dependent haemopoietic cells: Quantification by two flow cytometric methods. J. Immunol. Methods 153, 57-65 (1992).
    • (1992) J. Immunol. Methods , vol.153 , pp. 57-65
    • Ormerod, M.G.1    Collins, M.K.L.2    Rodriguez-Tarduchy, G.3    Robertson, D.4
  • 6
    • 0027209579 scopus 로고
    • Increased membrane permeability of apoptotic thymocytes: A flow cytometric study
    • Ormerod, M.G. et al. Increased membrane permeability of apoptotic thymocytes: A flow cytometric study. Cytometry 14, 595-602 (1993).
    • (1993) Cytometry , vol.14 , pp. 595-602
    • Ormerod, M.G.1
  • 7
    • 0025726216 scopus 로고
    • A rapid and simple method for measuring thymocyte apoptosis by propidium iodide staining and flow cytometry
    • Nicoletti, I., Migliorati, G., Pagliacci, M.C., Grignani, F. & Riccardi, C. A rapid and simple method for measuring thymocyte apoptosis by propidium iodide staining and flow cytometry. J. Immunol. Methods 139, 271-279 (1991).
    • (1991) J. Immunol. Methods , vol.139 , pp. 271-279
    • Nicoletti, I.1    Migliorati, G.2    Pagliacci, M.C.3    Grignani, F.4    Riccardi, C.5
  • 8
    • 0032411886 scopus 로고    scopus 로고
    • Mercuric chloride induces apoptosis in human T lymphocytes: Evidence of mitochondrial dysfunction
    • Guo, T.L., Miller, M.A., Shapiro, I.M. & Shenker, B.J. Mercuric chloride induces apoptosis in human T lymphocytes: Evidence of mitochondrial dysfunction. Toxicol. Appl. Pharmacol. 153, 250-257 (1998).
    • (1998) Toxicol. Appl. Pharmacol , vol.153 , pp. 250-257
    • Guo, T.L.1    Miller, M.A.2    Shapiro, I.M.3    Shenker, B.J.4
  • 9
    • 0018882157 scopus 로고
    • Separation of viable T and B lymphocytes using a cytochemical stain, Hoechst 33342
    • Loken, M.R. Separation of viable T and B lymphocytes using a cytochemical stain, Hoechst 33342. J. Histochem. Cytochem. 28 36-39 (1980).
    • (1980) J. Histochem. Cytochem , vol.28 , pp. 36-39
    • Loken, M.R.1
  • 10
    • 0026540818 scopus 로고
    • Comparative evaluation of several DNA binding dyes in the detection of apoptosis-associated chromatin degradation by flow cytometry
    • Telford, W.G., King, L.E. & Fraker, P.J. Comparative evaluation of several DNA binding dyes in the detection of apoptosis-associated chromatin degradation by flow cytometry. Cytometry 13, 137-143 (1992).
    • (1992) Cytometry , vol.13 , pp. 137-143
    • Telford, W.G.1    King, L.E.2    Fraker, P.J.3
  • 11
    • 0027931215 scopus 로고
    • Mitochondrial modifications during rat thymocyte apoptosis: A study at the single cell level
    • Cossarizza, A. et al. Mitochondrial modifications during rat thymocyte apoptosis: A study at the single cell level. Exp. Cell Res. 214, 323-330 (1994).
    • (1994) Exp. Cell Res , vol.214 , pp. 323-330
    • Cossarizza, A.1
  • 12
    • 0027269557 scopus 로고
    • Detection of DNA strand breaks in individual apoptotic cells by the in situ terminal deoxynucleotidyl transferase and nick translation assays
    • Gorczyca, W., Gong, J. & Darzynkiewicz, Z. Detection of DNA strand breaks in individual apoptotic cells by the in situ terminal deoxynucleotidyl transferase and nick translation assays. Cancer Res. 53, 1945-1951 (1993).
    • (1993) Cancer Res , vol.53 , pp. 1945-1951
    • Gorczyca, W.1    Gong, J.2    Darzynkiewicz, Z.3
  • 13
    • 0029043888 scopus 로고
    • A novel assay for apoptosis: Flow cytometric detection of phosphatidylserine expression on early apoptotic cells using fluorescein labelled Annexin V
    • Vermes, I., Haanen, C., Steffens-Nakken, H. & Reutellingsperger, C. A novel assay for apoptosis: Flow cytometric detection of phosphatidylserine expression on early apoptotic cells using fluorescein labelled Annexin V. J. Immunol. Methods 184, 39-51 (1995).
    • (1995) J. Immunol. Methods , vol.184 , pp. 39-51
    • Vermes, I.1    Haanen, C.2    Steffens-Nakken, H.3    Reutellingsperger, C.4
  • 14
    • 0034714009 scopus 로고    scopus 로고
    • Activation of caspases measured in situ by binding of fluorochrome-labeled inhibitors of caspases (FLICA): Correlation with DNA fragmentation
    • Bedner, E., Smolewski, P., Amstad, P. & Darzynkiewicz, Z. Activation of caspases measured in situ by binding of fluorochrome-labeled inhibitors of caspases (FLICA): Correlation with DNA fragmentation. Exp. Cell Res. 259, 308-313 (2000).
    • (2000) Exp. Cell Res , vol.259 , pp. 308-313
    • Bedner, E.1    Smolewski, P.2    Amstad, P.3    Darzynkiewicz, Z.4
  • 15
    • 0035234207 scopus 로고    scopus 로고
    • Difficulties and pitfalls in analysis of apoptosis
    • Darzynkiewicz, Z., Bedner, E. & Traganos, F. Difficulties and pitfalls in analysis of apoptosis. Methods Cell Biol. 63, 527-546 (2001).
    • (2001) Methods Cell Biol , vol.63 , pp. 527-546
    • Darzynkiewicz, Z.1    Bedner, E.2    Traganos, F.3
  • 16
    • 0022385290 scopus 로고
    • Flow-cytometric detection of changes in the fluorescence emission spectrumof a vital DNA-specific dye in human tumour cells
    • Smith, P.J., Nakeff, A. & Watson, J.V. Flow-cytometric detection of changes in the fluorescence emission spectrumof a vital DNA-specific dye in human tumour cells. Exp. Cell Res. 139, 37-46 (1985).
    • (1985) Exp. Cell Res , vol.139 , pp. 37-46
    • Smith, P.J.1    Nakeff, A.2    Watson, J.V.3
  • 17
    • 0028295351 scopus 로고
    • A rapid method for measuring apoptosis and dual-color immunofluorescence by single laser flow cytometry
    • Schmid, I., Uittenbogaart, C.H., Keld, B. & Giorgi, J.V. A rapid method for measuring apoptosis and dual-color immunofluorescence by single laser flow cytometry. J. Immunol. Methods 170, 145-157 (1994).
    • (1994) J. Immunol. Methods , vol.170 , pp. 145-157
    • Schmid, I.1    Uittenbogaart, C.H.2    Keld, B.3    Giorgi, J.V.4
  • 18
    • 0026556554 scopus 로고
    • Dead cell discrimination with 7-amino-actinomycin D in combination with dual color immunofluorescence in single laser flow cytometry
    • Schmid, I., Krall, W.J., Uittenbogaart, C.H., Braun, J. & Giorgi, J.V. Dead cell discrimination with 7-amino-actinomycin D in combination with dual color immunofluorescence in single laser flow cytometry. Cytometry 13, 204-208 (1992).
    • (1992) Cytometry , vol.13 , pp. 204-208
    • Schmid, I.1    Krall, W.J.2    Uittenbogaart, C.H.3    Braun, J.4    Giorgi, J.V.5
  • 19
    • 0032919403 scopus 로고    scopus 로고
    • Flow cytometric analysis of live cell proliferation and phenotype in populations with low viability
    • Schmid, I., Ferbas, J., Uittenbogaart, C.H. & Giorgi, J.V. Flow cytometric analysis of live cell proliferation and phenotype in populations with low viability. Cytometry 35, 64-74 (1999).
    • (1999) Cytometry , vol.35 , pp. 64-74
    • Schmid, I.1    Ferbas, J.2    Uittenbogaart, C.H.3    Giorgi, J.V.4


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.