Expression, purification, crystallization and preliminary X-ray diffraction analysis of the DDX3 RNA helicase domain

Acta Crystallographica Section F: Structural Biology and Crystallization Communications

Volumn 63, Issue 4, 2007, Pages 283-286

  Rodamilans, Bernardo ^a   Montoya, Guillermo ^a  

^a Structural Biology and Biocomputing Programme   (Spain)

Author keywords

DDX3; RNA helicases

Indexed keywords

ESCHERICHIA COLI;

COMPLEMENTARY DNA; DDX3X PROTEIN, HUMAN; DEAD BOX PROTEIN; UNCLASSIFIED DRUG;

ARTICLE; CHEMISTRY; CRYSTALLIZATION; ESCHERICHIA COLI; GENETICS; ISOLATION AND PURIFICATION; MOLECULAR CLONING; POLYACRYLAMIDE GEL ELECTROPHORESIS; POLYMERASE CHAIN REACTION; PROTEIN CONFORMATION; X RAY CRYSTALLOGRAPHY;

CLONING, MOLECULAR; CRYSTALLIZATION; CRYSTALLOGRAPHY, X-RAY; DEAD-BOX RNA HELICASES; DNA, COMPLEMENTARY; ELECTROPHORESIS, POLYACRYLAMIDE GEL; ESCHERICHIA COLI; POLYMERASE CHAIN REACTION; PROTEIN CONFORMATION;

EID: 34249723733     PISSN: 17443091     EISSN: 17443091     Source Type: Journal    
DOI: 10.1107/S1744309107006434     Document Type: Article

References (19)

1. Bradford, M. (1976). Anal. Biochem. 72, 248-254.
2. Caruthers, J. M, Johnson, E. R. & McKay, D. B. (2000). Proc. Natl Acad. Sci. USA, 97, 13080-13085.
3. Chang, P. C., Chi, C. W., Chau, G. Y., Li, F. Y., Tsai, Y. H., Wu, J. C. & Wu Lee, Y. H. (2006). Oncogene, 25, 1991-2003.
4. Chao, C. H., Chen, C. M., Cheng, P. L., Shih, J. W., Tsou, A. P. & Lee, Y. H. (2006). Cancer Res. 66, 6579-6588.
5. Cordin, O., Tanner, N. K., Doere,M., Linder, P. & Banroques, J. (2004). EMBO J. 23, 2478-2487.
6. Cruz, J., Kressler, D. & Linder, P. (1999). Trends Biochem. Sci. 24, 192-198.
7. Kanai, Y., Dohmae, N. & Hirokawa, N. (2004). Neuron, 43, 513-525.
8. Krishnan, V. & Zeichner, S. L. (2004). Retrovirology, 1, 42.
9. Lahn, B. & Page, D. (1997). Science, 278, 675-680.
10. Mamiya, N. & Worman, H. (1999). J. Biol. Chem. 274, 15751-15756.
11. Otwinowski, Z. & Minor, W. (1997). Methods Enzymol. 276, 307-326.
12. Owsianka, A. & Patel, A. (1999). Virology, 257, 330-340.
13. Rocak, S. & Linder, P. (2004). Nature Rev. Mol. Cell Biol. 5, 232-241.
14. Schwer, B. (2001). Nature Struct. Biol. 8, 113-116.
15. Sengoku, T., Nureki, O., Nakamura, A., Kobayashi, S. & Yokoyama, S. (2005). Cell, 125, 287-300.
16. Story, R. M., Li, H. & Abelson, J. N. (2001). Proc. Natl Acad. Sci. USA, 98, 1465-1470.
17. Yedavalli, V., Neuveut, C., Chi, Y., Kleiman, L. & Jeang, K. (2004). Cell, 119, 381-392.
18. You, L., Chen, C., Yeh, T., Tsai, T., Mai, R., Lin, C. & Lee, Y. (1999). J. Virol. 73, 2841-2853.
19. Zhou, Z., Licklider, L., Gygi, S. & Reed, R. (2002). Nature (London), 419, 182-185.