Tri-cistronic cloning, overexpression and purification of human Rad9, Rad1, Hus1 protein complex

Protein Expression and Purification

Volumn 54, Issue 2, 2007, Pages 204-211

  Singh, Vinay Kumar ^a   Nurmohamed, Salima ^a   Davey, Scott K ^a   Jia, Zongchao ^a  

^a QUEEN S UNIVERSITY   (Canada)

Author keywords

Affinity purification; ASEC; Cell cycle checkpoint control; DLS; DNA damage and repair; Heterologous expression; Hus1; Rad1; Rad9; Tri protein complex

Indexed keywords

CELL CYCLE PROTEIN; EXONUCLEASE; HUS1B PROTEIN, HUMAN; PROTEIN RAD9; RAD1 PROTEIN, HUMAN; UNCLASSIFIED DRUG;

AFFINITY CHROMATOGRAPHY; ARTICLE; ESCHERICHIA COLI; GEL CHROMATOGRAPHY; GENE VECTOR; GENETICS; ISOLATION AND PURIFICATION; MACROMOLECULE; METABOLISM; MOLECULAR CLONING; POLYACRYLAMIDE GEL ELECTROPHORESIS; WESTERN BLOTTING;

BLOTTING, WESTERN; CELL CYCLE PROTEINS; CHROMATOGRAPHY, AFFINITY; CHROMATOGRAPHY, GEL; CLONING, MOLECULAR; ELECTROPHORESIS, POLYACRYLAMIDE GEL; ESCHERICHIA COLI; EXONUCLEASES; GENETIC VECTORS; MACROMOLECULAR SUBSTANCES;

ESCHERICHIA COLI; SCHIZOSACCHAROMYCES POMBE;

EID: 34249102243     PISSN: 10465928     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.pep.2007.03.011     Document Type: Article

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