Selection strategy and the design of hybrid oligonucleotide primers for RACE-PCR: Cloning a family of toxin-like sequences from Agelena orientalis

BMC Molecular Biology

Volumn 8, Issue , 2007, Pages

  Pan, Zhensheng ^a   Barry, Richard ^a   Lipkin, Alexey ^b   Soloviev, Mikhail ^a  

^a UNIVERSITY OF LONDON   (United Kingdom)

^b SHEMYAKIN OVCHINNIKOV INSTITUTE OF BIOORGANIC CHEMISTRY   (Russian Federation)

Author keywords

[No Author keywords available]

Indexed keywords

MESSENGER RNA; SPIDER VENOM; COMPLEMENTARY DNA; PRIMER DNA; TOXIN;

3' UNTRANSLATED REGION; 5' UNTRANSLATED REGION; AMINO ACID SEQUENCE; ARTICLE; COMPUTER PROGRAM; EXPRESSED SEQUENCE TAG; GENE AMPLIFICATION; GENETIC IDENTIFICATION; GENETIC SELECTION; HIGH THROUGHPUT SCREENING; MOLECULAR CLONING; MULTIGENE FAMILY; NONHUMAN; NUCLEIC ACID HYBRIDIZATION; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; SPIDER; TOXIN ANALYSIS; ANIMAL; CHEMISTRY; CONSENSUS SEQUENCE; DNA SEQUENCE; GENETICS; METHODOLOGY; NUCLEOTIDE SEQUENCE; PHYLOGENY; POLYMERASE CHAIN REACTION; PROTEIN DATABASE; SEQUENCE ALIGNMENT; TRANSITION TEMPERATURE;

AGELENA; AGELENA ORIENTALIS; ARANEAE;

AMINO ACID SEQUENCE; ANIMALS; BASE SEQUENCE; CLONING, MOLECULAR; CONSENSUS SEQUENCE; DATABASES, PROTEIN; DNA PRIMERS; DNA, COMPLEMENTARY; PHYLOGENY; POLYMERASE CHAIN REACTION; SEQUENCE ALIGNMENT; SEQUENCE ANALYSIS, DNA; SPIDERS; TOXINS, BIOLOGICAL; TRANSITION TEMPERATURE;

EID: 34249030936     PISSN: None     EISSN: 14712199     Source Type: Journal    
DOI: 10.1186/1471-2199-8-32     Document Type: Article

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