A method for in vitro assembly of hepatitis C virus core protein and for screening of inhibitors

Analytical Biochemistry

Volumn 366, Issue 1, 2007, Pages 37-45

  Fromentin, Rémi ^a   Majeau, Nathalie ^a   Gagné, Marie Eve Laliberté ^a   Boivin, Annie ^a   Duvignaud, Jean Baptiste ^a   Leclerc, Denis ^a  

^a UNIVERSITÉ LAVAL   (Canada)

Author keywords

Absorbance spectrometry; Hepatitis C virus; In vitro assembly; Kinetics; Nucleocapsid

Indexed keywords

ELECTROSTATICS; ENZYME KINETICS; ESCHERICHIA COLI; NUCLEIC ACIDS; TURBIDITY;

ABSORBANCES; ASSEMBLY INHIBITORS; EXPERIMENTAL CONDITIONS; HEPATITIS C VIRUS; IN-VITRO; INHIBITORY PEPTIDES; MUTATIONAL ANALYSIS; NUCLEOCAPSIDS;

VIRUSES;

PEPTIDE DERIVATIVE; VIRUS PROTEIN;

ARTICLE; CONTROLLED STUDY; ESCHERICHIA COLI; HEPATITIS C VIRUS; HIGH THROUGHPUT SCREENING; IN VITRO STUDY; MUTATIONAL ANALYSIS; NONHUMAN; PRIORITY JOURNAL; RADIOACTIVITY; SPECTROPHOTOMETRY; TURBIDITY; VIRUS ASSEMBLY; VIRUS CORE; VIRUS NUCLEOCAPSID;

ESCHERICHIA COLI; HEPATITIS C VIRUS;

EID: 34248548509     PISSN: 00032697     EISSN: 10960309     Source Type: Journal    
DOI: 10.1016/j.ab.2007.03.033     Document Type: Article

References (26)

1. Thomson B.J., and Finch R.G. Hepatitis C virus infection. Clin. Microbiol. Infect. 11 (2005) 86-94
2. Matsuura Y., and Miyamura T. The molecular biology of hepatitis C virus. Semin. Virol. 4 (1993) 297-304
3. Saracco G., Olivero A., Ciancio A., Carenzi S., and Rizzetto M. Therapy of chronic hepatitis C: A critical review. Curr. Drug Targets Infect. Disord. 3 (2003) 25-32
4. McLauchlan J., Lemberg M.K., Hope G., and Martoglio B. Intramembrane proteolysis promotes trafficking of hepatitis C virus core protein to lipid droplets. EMBO J. 21 (2002) 3980-3988
5. Ogino T., Fukuda H., Imajoh-Ohmi S., Kohara M., and Nomoto A. Membrane binding properties and terminal residues of the mature hepatitis C virus capsid protein in insect cells. J. Virol. 78 (2004) 11766-11777
6. Suzuki R., Tamura K., Li J., Ishii K., Matsuura Y., Miyamura T., and Suzuki T. Ubiquitin-mediated degradation of hepatitis C virus core protein is regulated by processing at its carboxyl terminus. Virology 280 (2001) 301-309
7. Shih C., Chen C., Chen S., and Lee Y. Modulation of the trans-suppression activity of hepatitis C virus core protein by phosphorylation. J. Virol. 69 (1995) 1160-1171
8. Majeau N., Bolduc M., Duvignaud J.-B., Fromentin R., and Leclerc D. Effect of cAMP-dependent protein kinase A (PKA) on HCV nucleocapsid assembly and degradation. Biochem. Cell Biol. 85 (2007) 78-87
9. van der Schoot P., and Bruinsma R. Electrostatics and the assembly of an RNA virus. Phys. Rev. E 71 (2005) 061928
10. Kunkel M., Lorinczi M., Rijnbrand R., Lemon S.M., and Watowich S.J. Self-assembly of nucleocapsid-like particles from recombinant hepatitis C virus core protein. J. Virol. 75 (2001) 2119-2129
11. Shimoike T., Mimori S., Tani H., Matsuura Y., and Miyamura T. Interaction of hepatitis C virus core protein with viral sense RNA and suppression of its translation. J. Virol. 73 (1999) 9718-9725
12. Tanaka Y., Shimoike T., Ishii K., Suzuki R., Suzuki T., Ushijima H., Matsuura Y., and Miyamura T. Selective binding of hepatitis C virus core protein to synthetic oligonucleotides corresponding to the 5′ untranslated region of the viral genome. Virology 270 (2000) 229-236
13. Bukh J., Purcell R.H., and Miller R.H. Sequence analysis of the 5′ noncoding region of hepatitis C virus. Proc. Natl. Acad. Sci. USA 89 (1992) 4942-4946
14. Honda M., Beard M.R., Ping L.-H., and Lemon S.M. A phylogenetically conserved stem-loop structure at the 5′ border of the internal ribosome entry site of hepatitis C virus is required for cap-independent viral translation. J. Virol. 73 (1999) 1165-1174
15. Beales L.P., Rowlands D.J., and Holzenburg A. The internal ribosome entry site (IRES) of hepatitis C virus visualized by electron microscopy. RNA 7 (2001) 661-670
16. Piron M., Beguiristain N., Nadal A., Martinez-Salas E., and Gomez J. Characterizing the function and structural organization of the 5′ tRNA-like motif within the hepatitis C virus quasispecies. Nucleic Acids Res. 33 (2005) 1487-1502
17. Tsukiyama-Kohara K., Iizuka N., Kohara M., and Nomoto A. Internal ribosome entry site within hepatitis C virus RNA. J. Virol. 66 (1992) 1476-1483
18. Majeau N., Gagne V., Boivin A., Bolduc M., Majeau J.-A., Ouellet D., and Leclerc D. The N-terminal half of the core protein of hepatitis C virus is sufficient for nucleocapsid formation. J. Gen. Virol. 85 (2004) 971-981
19. Ikemura T. Correlation between the abundance of Escherichia coli transfer RNAs and the occurrence of the respective codons in its protein genes: A proposal for a synonymous codon choice that is optimal for the E. coli translational system. J. Mol. Biol. 151 (1981) 389-409
20. Rijnbrand R., Bredenbeek P.J., Haasnoot P.C., Kieft J.S., Spaan W.J., and Lemon S.M. The influence of downstream protein-coding sequence on internal ribosome entry on hepatitis C virus and other flavivirus RNAs. RNA 7 (2001) 585-597
21. Tellinghuisen T.L., Hamburger A.E., Fisher B.R., Ostendorp R., and Kuhn R.J. In vitro assembly of alphavirus cores by using nucleocapsid protein expressed in Escherichia coli. J. Virol. 73 (1999) 5309-5319
22. Bancroft J.B., Hiebert E., and Bracker C.E. The effects of various polyanions on shell formation of some spherical viruses. Virology 39 (1969) 924-930
23. Klein K., Dellos S., and Lingappa J. Identification of residues in the hepatitis C virus core protein that are critical for capsid assembly in a cell-free system. J. Virol. 79 (2005) 6814-6826
24. Sticht J., Humbert M., Findlow S., Bodem J., Muller B., Dietrich U., Werner J., and Krausslich H.-G. A peptide inhibitor of HIV-1 assembly in vitro. Nat. Struct. Mol. Biol. 12 (2005) 671-677
25. Rodriguez-Casado M.M.P.C.A. Conformational features of truncated hepatitis C virus core protein in virus-like particles. Biopolymers 82 (2006) 334-338
26. Zlotnick A., Ceres P., Singh S., and Johnson J.M. A small molecule inhibits and misdirects assembly of hepatitis B virus capsids. J. Virol. 76 (2002) 4848-4854