Co-bombardment, integration and expression of rice chitinase and thaumatin-like protein genes in barley (Hordeum vulgare cv. Conlon)

Plant Cell Reports

Volumn 26, Issue 5, 2007, Pages 631-639

  Tobias, Dennis J ^a   Manoharan, Muthusamy ^b   Pritsch, Clara ^c   Dahleen, Lynn S ^d  

^a NORTH DAKOTA STATE UNIVERSITY   (United States)

^b UNIVERSITY OF ARKANSAS AT PINE BLUFF   (United States)

^c UNIVERSIDAD DE LA REPÚBLICA   (Uruguay)

^d AGRICULTURAL RESEARCH SERVICE   (United States)

Author keywords

Oryza sativa; PR proteins; Transgene expression; Transgene silencing

Indexed keywords

ORYZA SATIVA; PR PROTEINS; TRANSGENE EXPRESSION; TRANSGENE SILENCING;

ENZYMES; FUNGI; GENE EXPRESSION;

CROPS;

CHITINASE; MESSENGER RNA; PLANT DNA; THAUMATIN PROTEIN, PLANT; UNCLASSIFIED DRUG; VEGETABLE PROTEIN;

ARTICLE; BARLEY; CHI SQUARE DISTRIBUTION; CHROMOSOME SEGREGATION; CLASSIFICATION; ENZYMOLOGY; GENE; GENE EXPRESSION REGULATION; GENETICS; GENOME; METABOLISM; PLANT LEAF; PLASMID; RICE; SOUTHERN BLOTTING; TRANSGENIC PLANT; WESTERN BLOTTING;

BLOTTING, SOUTHERN; BLOTTING, WESTERN; CHI-SQUARE DISTRIBUTION; CHITINASE; CHROMOSOME SEGREGATION; DNA, PLANT; GENE EXPRESSION REGULATION, ENZYMOLOGIC; GENE EXPRESSION REGULATION, PLANT; GENES, PLANT; GENOME, PLANT; HORDEUM; ORYZA SATIVA; PLANT LEAVES; PLANT PROTEINS; PLANTS, GENETICALLY MODIFIED; PLASMIDS; RNA, MESSENGER;

BARLEY; ENZYMES; FUNGI; GENES; RICE;

FUNGI; HORDEUM; HORDEUM VULGARE; HORDEUM VULGARE SUBSP. VULGARE; ORYZA SATIVA; ZEA MAYS;

EID: 34247607316     PISSN: 07217714     EISSN: None     Source Type: Journal    
DOI: 10.1007/s00299-006-0263-x     Document Type: Article

References (47)

1. Abad LR, D'Urzo MP, Liu D, Narasimhan ML, Reuveni M, Zhu JK, Niu X, Singh NK, Hasegawa PM, Bressan RA (1996) Antifungal activity of tobacco osmotin has specificity and involves plasma membrane permeabilization. Plant Sci 118:11-23
2. Anand A, Zhou T, Trick HN, Gill BS, Bockus WW, Muthukrishnan S (2003a) Greenhouse and field testing of transgenic wheat plants stably expressing genes for thaumatin-like protein, chitinase and glucanase against Fusarium graminearum. J Exp Bot 54:1101-1111
3. Anand A, Trick HN, Gill BS, Muthukrishnan S (2003b) Stable transgene expression and random silencing in wheat. Plant Biotechnol J 1:241-251
4. Bradford MM (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 72:248-254
5. Bregitzer P, Zhang S, Cho MJ, Lemaux PG (2002) Reduced somaclonal variation in barley is associated with culturing highly differentiated, meristematic tissues. Crop Sci 42:1303-1308
6. Broglie K, Chet I, Holliday M, Cressman R, Biddle P, Knowlton S, Mauvais CJ, Broglie R (1991) Transgenic plants with enhanced resistance to the fungal pathogen Rhizoctonia solani. Science 254:1194-1197
7. Chen WP, Gu X, Liang GH, Muthukrishnan S, Chen PD, Liu DJ, Gill BS (1998) Introduction and constitutive expression of a rice chitinase gene in bread wheat using biolistic bombardment and the bar gene as a selectable marker. Theor Appl Genet 97:1296-1306
8. Chen WP, Chen PD, Liu DJ, Kynast R, Friebe B, Velazhahan R, Muthukrishnan S, Gill BS (1999) Development of wheat scab symptoms is delayed in transgenic wheat plants that constitutively express a rice thaumatin-like protein gene. Theor Appl Genet 99:755-760
9. Dahleen LS, Okubara PA, Blechl AE (2001) Transgenic approaches to combat Fusarium head blight in wheat and barley. Crop Sci 41:628-637
10. Dahleen LS, Bregitzer P (2002) An improved media system for high regeneration rates from barley immature embryo-derived callus of commercial cultivars. Crop Sci 42:934-938
11. Datta K, Velazhahan R, Oliva N, Ona I, Mew T, Khush GS, Muthukrishnan S, Datta SK (1999) Over-expression of the cloned rice thaumatin-like protein (PR-5) gene in transgenic rice plants enhances environmental friendly resistance to Rhizoctonia solani causing sheath blight disease. Theor Appl Genet 98:1138-1145
12. Datta K, Tu J, Oliva N, Ona I, Velazhahan R, Mew TW, Muthukrishnan S, Datta SK (2001) Enhanced resistance to sheath blight by constitutive expression of infection-related rice chitinase in transgenic elite indica rice cultivars. Plant Sci 160:405-414
13. Fu X, Kohli A, Twyman RM, Christou P (2000) Alternative silencing effects involve distinct types of non-spreading cytosine methylation at a three-gene, single-copy transgenic locus in rice. Mol Gen Genet 263:106-118
14. Grison R, Grezes-BassetB, ScneiderM, Lucante N, Olsen L, Leguay JJ, Toppan A (1996) Field tolerance to fungal pathogens of Brassica napus constitutively expressing a chimeric chitinase gene. Nat Biotechnol 14:643-646
15. Horvath H, Rostoks N, Brueggeman R, Steffenson B, vonWettstein D, Kleinhofs A (2003) Genetically engineered stem rust resistance in barley using Rpg1 gene. Proc Natl Acad Sci USA 100:364-369
16. Hu X, Reddy ASN (1997) Cloning and expression of a PR-5-like protein from Arabidopsis: inhibition of fungal growth by bacterially-expressed protein. Plant Mol Biol 34:949-959
17. Ignatius SMJ, Huang JK, Chopra RK, Muthukrishnan S (1994) Isolation and characterization of a barley chitinase genomic clone: expression in powdery mildew infected barley. J Plant Biochem Biotechnol 3:91-95
18. Iyer LM, Kumpatla SP, Chandrasekharan MB, Hall TC (2000) Transgene silencing in monocots. Plant Mol Biol 43:323-346
19. Jach G, Gornhardt B, Mundy J, Logemann J, Pinsdorf E, Leah R, Schell J, Mass C (1995) Enhanced quantitative resistance against fungal disease by combinatorial expression of different barley antifungal proteins in transgenic tobacco. Plant J 8:97-109
20. Jongedijk E, Tigelaar H, Van Roeckel JSC, Bres-Vloemans SA, Dekker I, van den Elzen PJM, Cornelissen BJC, Melchers LS (1995) Synergistic activity of chitinases and β-1,3-glucanases enhances fungal resistance in transgenic tomato plants. Euphytica 85:173-180
21. Kim JK, Jang IC, Wu R, Zuo WN, Boston RS, Lee YH, Ahn IP, Nahm BH (2003) Co-expression of a modified maize ribosome-inactivating protein and a rice chitinase gene in transgenic rice plants confers enhanced resistance to sheath blight. Transgenic Res 12:475-484
22. Kohli A, Gahakwa D, Vain P, Laurie DA, Christou P (1999) Transgene expression in rice engineered through particle bombardment: molecular factors controlling stable expression and gene silencing. Planta 208:88-97
23. Kumpatla SP, Hall TC (1999) Organizational complexity of a rice transgene locus susceptible to methylation-based silencing. IUBMB Life 48:459-467
24. Laemmli UK (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227:680-685
25. Leah R, Tommerup H, Svendsen I, Mundy J (1991) Biochemical and molecular characterization of three barley seed proteins with antifungal properties. J Biol Chem 266:1464-1473
26. Lemaux PG, Cho MJ, Zhang S, Bregitzer P (1999) Transgenic cereals: Hordeum vulgare L. (barley). In: Vasil IK (ed) Molecular improvement of cereal crops. Kluwer Academic Publishers, Dordrecht, The Netherlands, pp 255-316
27. Lin W, Anuratha CS, Datta K, Potrykus I, Muthukrishnan S, Datta SK (1995) Genetic engineering of rice for resistance to sheath blight. Bio/Technology 13:686-691
28. Manoharan M, Dahleen LS (2002) Genetic transformation of the commercial barley (Hordeum vulgare L.) cultivar Conlon by particle bombardment of callus. Plant Cell Rep 21:76-80
29. Marchant R, Davey MR, Lucas JA, Lamb CJ, Dixon RA, Power JB (1998) Expression of a chitinase transgene in rose (Rosa hybrida L.) reduces development of black spot disease (Diplocarpon rosae Wolf.). Mol Breed 4:187-194
30. Matzke MA, Matzke AJM (1995) How and why do plants inactivate homologous transgenes? Plant Physiol 107:679-685
31. Mauch F, Mauch-Mani B, Boller T (1988) Antifungal hydrolases in pea tissue: inhibition of fungal growth by combinations of chitinase and β-1,3-glucanase. Plant Physiol 88:936-942
32. Meng L, Bregitzer P, Zhang S, Lemaux P (2003) Methylation of the exon/intron region in the Ubi1 promoter complex correlates with transgene silencing in barley. Plant Mol Biol 53:327-340
33. Meyer P, Saedler H (1996) Homology dependent gene silencing in plants. Ann Rev Plant Physiol Plant Mol Biol 47:23-48
34. Murashige T, Skoog FA (1962) A revised medium for rapid growth and bioassays with tobacco tissue culture. Plant Physiol 15:473-497
35. Murray MG, Thompson WF (1980) Rapid isolation of high molecular weight plant DNA. Nucleic Acids Res 8: 4321-4325
36. Oldach KH, Becker D, Lorz H (2001) Heterologous expression of genes mediating enhanced fungal resistance in transgenic wheat. Mol Plant Microbe Interact 14:832-838
37. Roberts WK, Selitrennikoff CP (1990) Zeamatin, an antifungal protein from maize with membrane-permeabilizing activity. J Gen Microbiol 136:1771-1778
38. Schlumbaum A, Mauch F, Vogeli U, Boller T (1986) Plant chitinases are potent inhibitors of fungal growth. Nature 324:365-367
39. Tabei Y, Kitade S, Nishizawa Y, Kikuchi N, Kayano T, Hibi T, Akutsu K (1998) Transgenic cucumber plants harboring a rice chitinase gene exhibit enhanced resistance to gray mold (Botrytis cinerea). Plant Cell Rep 17:159-164
40. Terakawa T, Takaya N, Horiuchi H, Koike M, Takagi M (1997) A fungal chitinase gene from Rhizopus oligosporus confers antifungal activity to transgenic tobacco. Plant Cell Rep 16:439-443
41. Thompson CJ, Novva NR, Tizard T, Crameri R, Davies JE, Lauwereys M, Botterman J (1987) Characterization of the herbicide-resistance gene bar from Streptomyces hygroscopicus. EMBO J 6:2519-2523
42. Vaucheret H, Beclin C, Elmayan T, Feuerbach F, Godon C, Morel JB, Mourrain P, Palauqui JC, Vernhettes S (1998) Transgene-induced gene silencing in plants. Plant J 16:651-659
43. Velazhahan R, Datta SK, Muthukrishnan S (1999) The PR-5 family; thaumatin-like proteins. In: Datta SK, Muthukrishnan S (eds) Pathogenesis-related proteins in plants. CRC Press, Boca Raton, FL, pp 107-129
44. Velazhahan R, Samiyappan R, Vidhyasekaran P (2000) Purification of an elicitor-inducible antifungal chitinase from suspension-cultured rice cells. Phytoparasitica 28:131-139
45. Velazhahan R, Muthukrishnan S (2003) Transgenic tobacco plants constitutively overexpressing a rice thaumatin-like protein (PR-5) show enhanced resistance to Alternaria alternate. Biol Plantarum 47:347-354
46. Vigers AJ, Roberts WK, Selitrennikoff CP (1991) A new family of plant antifungal proteins. Mol Plant Microbe Interact 4:315-323
47. Zhu Q, Maher EA, Masoud S, Dixon RA, Lamb CJ (1994) Enhanced protection against fungal attack by constitutive expression of chitinase and glucanase genes in transgenic tobacco. Bio/Technology 12:807-812