Cloning and expression of a PR5-like protein from Arabidopsis: Inhibition of fungal growth by bacterially expressed protein

Plant Molecular Biology

Volumn 34, Issue 6, 1997, Pages 949-959

  Hu, Xu ^a,b   Reddy, A S N ^a  

^a Department of Environmental and Radiological Health Sciences   (United States)

^b NATIONAL RESEARCH COUNCIL CANADA   (Canada)

Author keywords

Antifungal activity; Arabidopsis; Osmotin; Pathogenesis related protein; Plant defense; PR5 protein; Thaumatin like protein

Indexed keywords

ANTIFUNGAL AGENT; COMPLEMENTARY DNA; PATHOGENESIS RELATED PROTEINS, PLANT; PATHOGENESIS-RELATED PROTEINS, PLANT; RECOMBINANT PROTEIN; SWEETENING AGENT; THAUMATIN PROTEIN, PLANT; VEGETABLE PROTEIN;

AMINO ACID SEQUENCE; ARABIDOPSIS; ARTICLE; BIOSYNTHESIS; CANDIDA ALBICANS; COMPARATIVE STUDY; DEUTEROMYCETES; DNA SEQUENCE; DRUG EFFECT; ESCHERICHIA COLI; GENETICS; MICROBIOLOGICAL EXAMINATION; MOLECULAR CLONING; MOLECULAR GENETICS; NORTHERN BLOTTING; NUCLEOTIDE SEQUENCE; PLANT DISEASE; SEQUENCE HOMOLOGY; SOUTHERN BLOTTING;

AMINO ACID SEQUENCE; ANTIFUNGAL AGENTS; ARABIDOPSIS; BASE SEQUENCE; BLOTTING, NORTHERN; BLOTTING, SOUTHERN; CANDIDA ALBICANS; CLONING, MOLECULAR; DNA, COMPLEMENTARY; ESCHERICHIA COLI; MICROBIAL SENSITIVITY TESTS; MITOSPORIC FUNGI; MOLECULAR SEQUENCE DATA; PLANT DISEASES; PLANT PROTEINS; RECOMBINANT PROTEINS; SEQUENCE ANALYSIS, DNA; SEQUENCE HOMOLOGY, AMINO ACID; SWEETENING AGENTS;

ARABIDOPSIS; CANDIDA ALBICANS; ESCHERICHIA; ESCHERICHIA COLI; FUNGI IMPERFECTI;

EID: 0031213622     PISSN: 01674412     EISSN: None     Source Type: Journal    
DOI: 10.1023/A:1005893119263     Document Type: Article

References (48)

1. Abad LR, D'Urzo MP, Liu D, Narasimhan M, Reuveni M, Zhu JK, Niu X, Singh NK, Hasegawa P, Bressan R: Antifungal activity of tobacco osmotin has specificity and involves membrane permeabilization. Plant Sci 118: 11-23 (1996).
2. Batalia MA, Monzingo AF, Ernst S, Roberts W, Robertus JD: The crystal structure of the antifungal protein zeamatin, a member of a thaumatin-like, PR-5 protein family. Nature Struct Biol 3: 19-23 (1996).
3. Bol JF, Linthorst HJM, Cornellissen BJC: Plant pathogenesis-related proteins induced by virus infection. Annu Rev Phytopath 28: 113-138 (1990).
4. Bradford MM: A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 72: 148-254 (1976).
5. Bryngelsson T, Green B: Characterization of a pathogenesis-related, thaumatin-like protein isolated from barely challenged with an incompatible race of mildew. Physiol Mol Plant Path 35: 45-52 (1989).
6. Cammue BPA, De Bolle MFC, Terras FRG, Prrost P, Damme JV, Rees SB, Vanderleyden J, Broekaert WF: Isolation and characterization of a novel class of plant antimicrobial peptides from Mirabilis jalapa L. seeds. J Biol Chem 267: 2228-2233 (1992).
7. Caruso C, Caporale C, Chilosi G, Vacca F, Bertini L, Magro P, Poerio E, Buonocore V: Structural and antifungal properties of a pathogenesis-related protein from wheat kernel. J Protein Chem 15: 35-44 (1996).
8. Church GM, Gilbert W: Genomic sequencing. Proc Natl Acad Sci USA 81: 1991-1995 (1984).
9. Cornelissen BJC, Hooft van Huijsduijnen RAM, Bol JF: A tobacco mosaic virus-induced protein is homologous to the sweet-tasting protein thaumatin. Nature 321: 531-532 (1986).
10. Delaney TP, Uknes S, Vernooij B, Friedrich L, Weymann K, Negrotto D, Gaffney T, Gut-Rella M, Kessmann H, Ward E, Ryals J: A central role of salicylic acid in plant disease resistance. Science 266: 1247-1250 (1994).
11. Edens L, Hesling L, Klock R, Ledeboer AM, Maat J, Toonen MY, Visser C, Verrips CT: Cloning of cDNA encoding the sweet-tasting plant protein thaumatin and its expression in Escherichia coli. Gene 18: 1-12 (1982).
12. Frendo P, Didierjean J, Passelegue E, Burkard G: Abiotic stresses induce a thaumatin-like protein in maize; cDNA isolation and sequence analysis. Plant Sci 85: 61-69 (1992).
13. Gaffney T, Friedrich L, Vernooij B, Negrotto D, Nye D, Uknes S, Ward E, Kessmann H, Ryals J: Requirement of salicylic acid for the induction of systemic acquired resistance. Science 261: 754-756 (1993).
14. Golovkin M, Reddy ASN: Structure and expression of a plant U1 snRNP 70K gene: Alternative splicing of U1 snRNP 70K pre-mRNAs produces two different transcripts. Plant Cell 8: 1421-1435 (1996).
15. Hajgaard J, Jacobsen S, Svendsen I: Two antifungal thaumatin-like proteins from barley grain. FEBS Lett 291: 127-131 (1991).
16. Hu X, Nazar RN, Robb J: Quantification of Verticillium biomass in wilt disease development. Physiol Mol Path 42: 23-36 (1993).
17. Hu X, Reddy ASN: Nucleotide sequence of a cDNA clone encoding a thaumatin-like protein from Arabidopsis. Plant Physiol 107: 305-306 (1995).
18. Huynh QK, Borgmeyer JR, Zobel JF: Isolation and characterization of a 22 kDa protein with antifungal properties from maize seeds. Biochem Biophys Res Commun 182: 1-5 (1992).
19. Kauffmann S, Legrand M, Geoffroy P, Fritig B: Biological function of 'pathogenesis-related' proteins: four PR proteins of tobacco have 1,3-β glucanase activity. EMBO J 6: 3209-3212 (1987).
20. King EO, Ward MK, Raney DE: Two simple media for the demonstration of phycocyanin and fluorescin. J Lab Clin Med 44: 301-307 (1954).
21. King GJ, Turner VA, Hussey VE, Wurtele ES, Lee SM: Isolation and characterization of a tomato cDNA clone which codes for a salt-induced protein. Plant Mol Biol 10: 401-412 (1988).
22. La Rosa PC, Handa AK, Hasegawa PM, Bressan RA: Abscisic acid accelerates adaptation of cultured cells to salt. Plant Physiol 79: 138-142 (1985).
23. Legrand M, Kauffmann S, Geoffroy P, Fritig B: Biological function of 'pathogenesis-related' proteins: four tobacco PR-proteins are chitinases. Proc Natl Acad Sci USA 84: 6750-6754 (1987).
24. Linthorst HJM: Pathogenesis-related proteins of plants. CRC Cri Rev Plant Sci 10: 123-150 (1991).
25. Liu D, Raghothama KG, Hasegawa PM, Bressan RA: Osmotin overexpression in potato delays development of disease symptoms. Proc Natl Acad Sci USA 91: 1888-1892 (1994).
26. Malehorn D, Borgmeyer JR, Smith CE, Shah DM: Characterization and expression of an antifungal zeamatin-like protein (Zlp) gene from Zea mays. Plant Physiol 106: 1471-1481 (1994).
27. Mauch F, Mauch-Mani B, Boller T: Antifungal hydrolases in pea tissue. II. Inhibition of fungal growth by combinations of chitinases and β-1,3 glucanase. Plant Physiol 88: 936-942 (1988).
28. Memelink J, Linthorst HJM, Schilperoort RA, Hoge HC: Tobacco genes encoding acidic and basic pathogenesis-related proteins display different expression pattern. Plant Mol Biol 14: 119-126 (1990).
29. Payne G, Middlesteadt W, Williams S, Desai N, Parks TD, Dincher S, Carnes M, Ryals J: Isolation and nucleotide sequence of a novel cDNA clone encoding the major form of pathogenesis-related protein R. Plant Mol Biol 11: 223-224 (1988).
30. Pierpoint WS, Jackson PJ, Evans RM: The presence of a thaumatin-like protein, a chitinase and a glucanase among the pathogenesis-related proteins of potato (Solanum tuberosum). Physiol Mol Plant Path 36: 325-338 (1990).
31. Ponstein AS, Bres-vloemans SA, Sela-Buurlage MB, van den Elzen PJM, Melchers LS, Cornelissen BJC: A novel pathogenand wound-inducible tobacco (Nicotiana tabacum) protein with antifungal activity. Plant Physiol 104: 109-118 (1994).
32. Rebmann G, Mauch F, Dudler R: Sequence of a wheat cDNA encoding a pathogen-induced thaumatin-like protein. Plant Mol Biol 17: 283-285 (1991).
33. Reddy ASN, Safadi F, Narasimhulu S, Golovkin M, Hu X: A novel plant calmodulin-binding protein with a kinesin heavy chain motor domain. J Biol Chem 12: 7052-7060 (1996).
34. Reimmann C, Dudler R: cDNA cloning and sequence analysis of a pathogen-induced thaumatin-like protein from rice (Oryza saliva). Plant Physiol 101: 1113-1114 (1993).
35. Roberts WK, Selitrennikoff CP: Zeamatin, an antifungal protein from maize with membrane permeabilizing activity. J Gen Microbiol 136: 1771-1778 (1990).
36. Sambrook J, Fritsch EF, Maniatis T: Molecular Cloning: A Laboratory Manual. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY (1989).
37. Sanger F, Nicklen S, Coulson AR: DNA sequencing with chain terminating inhibitors. Proc Natl Acad Sci USA 74: 5463-5467 (1977).
38. Singh N, Nelson D, Kuhn D, Hasegawa P, Bressan R: Molecular cloning of osmotin and regulation of its expression by ABA and adaptation to low water potential. Plant Physiol 90: 1096-1101 (1989).
39. Singh NK, Bracker CA, Hasegawa PM, Handa AK, Buckel S, Hermodson MA, Pfankoch E, Regnier FE, Bressan RA: Characterization of osmotin: a thaumatin-like protein associated with osmotic adaptation in plant cells. Plant Physiol 85: 529-536 (1987).
40. Uknes S, Mauch-Mani B, Moyer M, Potter S, Williams S, Dincher S, Chandler D, Slusarenko A, Ward E, Ryals J: Acquired resistance in Arabidopsis. Plant Cell 4: 645-656 (1992).
41. van der Wel H, Loeve K: Isolation and characterization of thaumatin I and II, the sweet tasting proteins from Thaumatococcus danielli Benth J Biochem 31: 221-225 (1972).
42. van Kan JAL, van de Rhee MD, Zuidema D, Cornelissen BJC, Bol JF: Structure of tobacco genes encoding thaumatin-like proteins. Plant Mol Biol 12: 153-155 (1989).
43. van Loon LC, Gerritsen YAM, Ritter CE: Identification, purification and characterization of pathogenesis-related proteins from virus-infected Samsun NN tobacco leaves. Plant Mol Biol 14: 381-392 (1987).
44. Vigers AJ, Roberts WK, Selitrennikoff CP: A new family of plant antifungal proteins. Mol Plant-Microbe Interact 4: 315-323 (1991).
45. Vigers AJ, Wiedemann S, Roberts WK, Legrand M, Selitrennikoff CP, Fritig B: Thaumatin-like pathogenesis-related proteins are antifungal. Plant Sci 83: 155-161 (1992).
46. Wang X, Zafian P, Choudhary M, Lawton M: The PR5K receptor protein kinase from Arabidopsis thaliana is structurally related to a family of plant defense proteins. Proc Natl Acad Sci USA 93: 2598-2602 (1996).
47. Ward ER, Uknes SJ, Williams SC, Dincher SS, Wiederhold DL, Alexander DC, Ahl-Goy P, Metraux JP, Ryals JA: Coordinate gene activity in response to agents that induce systemic acquired resistance. Plant Cell 3: 1085-1094 (1991).
48. Woloshuk CP, Meulenhoff EJS, Sela-Buurlage M, van den Elzen PJM, Cornelissen BJC: Pathogen-induced proteins with inhibitory activity toward Phytophthora infestans. Plant Cell 3: 619-628 (1991).