Transgenic tobacco plants constitutively overexpressing a rice thaumatin-like protein (PR-5) show enhanced resistance to Alternaria alternata

Biologia Plantarum

Volumn 47, Issue 3, 2003, Pages 347-354

  Velazhahan, R ^a   Muthukrishnan, S ^b  

^a TAMIL NADU AGRICULTURAL UNIVERSITY   (India)

^b KANSAS STATE UNIVERSITY   (United States)

Author keywords

Disease resistance; Nicotiana tabacum; Pathogenesis related proteins

Indexed keywords

AGROBACTERIUM; ALTERNARIA; ALTERNARIA ALTERNATA; NICOTIANA; NICOTIANA OBTUSIFOLIA; NICOTIANA TABACUM; PHASEOLUS (ANGIOSPERM); PUNCTUM BLANDIANUM; RHIZOBIUM;

EID: 0842270131     PISSN: 00063134     EISSN: None     Source Type: Journal    
DOI: 10.1023/B:BIOP.0000023876.55053.5e     Document Type: Article

References (41)

1. An, G.: Binary Ti vectors for plant transformation and promoter analysis. - Methods Enzymol. 153: 292-305, 1987.
2. Ausubel, F.M., Brent, R., Kingston, R.E., Moore, D.D., Seidman, J.G., Smith, J.A., Struhl, K.: Current Protocols in Molecular Biology. - John Wiley and Sons, New York 1987.
3. Bent, A.F., Yu, I.: Applications of molecular biology to plant disease and insect resistance. - Adv. Agron. 66: 251-298, 1999.
4. Bol, J.F., Linthorst, H.J.M., Cornelissen, B.J.C.: Plant pathogenesis-related proteins induced by virus infection. -Annu. Rev. Phytopathol. 28: 113-138, 1990.
5. Bradford, M.M.: A rapid and sensitive method for the quantification of microgram quantities of protein utilizing the principle of protein-dye binding. - Anal. Biochem. 72: 248-254, 1976.
6. Cammue, B.P.A., Thevissen, K., Hendriks, M., Eggermont, K., Goderis, I.J., Proost, P., Van Damme, J., Osborn, R.W., Guerbette, F., Kader, J.C., Broekaert, W.F.: A potent antimicrobial protein from onion seeds showing sequence homology to plant lipid transfer proteins. - Plant Physiol. 109: 445-455, 1995.
7. Carmona, M.J., Hernandez-Lucas, C., San Martin, C., Gonzalez, P., Garcia-Olmedo, F.: Subcellular localization of type I thionins in the endosperm of wheat and barley. - Protoplasma 173: 1-7, 1993.
8. Chen, W.P., Chen, P.D., Liu, D.J., Kynast, R., Friebe, B., Velazhahan, R., Muthukrishnan, S., Gill, B.S.: Development of wheat scab symptoms is delayed in transgenic wheat plants that constitutively express a rice thaumatin-like protein gene. - Theor. appl. Genet. 99: 755-760, 1999.
9. Choi, Y.O., Cheong, N.E., Kim, W.Y., Lee, K.O., Moon, H.J., Cho, M.J., Lee, S.Y.: Isolation and biochemical properties of an antifungal thaumatin-like protein from flower buds of Chinese cabbage. - Korean J. Plant Pathol. 13: 386-393, 1997.
10. Cornelissen, B.J.C., Melchers, L.S.: Strategies for control of fungal diseases with transgenic plants. - Plant Physiol. 101: 709-712, 1993.
11. Datta, K., Velazhahan, R., Oliva, N., Ona, I., Mew, T., Khush, G.S., Muthukrishnan, S., Datta, S.K.: Overexpression of cloned rice thaumatin-like protein (PR-5) gene in transgenic rice plants enhances environmental friendly resistance to Rhizoctonia solani causing sheath blight disease. - Theor. appl. Genet. 98: 1138-1145, 1999.
12. Dore, I., Legrand, M., Cornelissen, B.J.C., Bol, J.F.: Subcellular localization of acidic and basic PR proteins in tobacco mosaic virus-infected tobacco. - Arch. Virol. 120: 97-107, 1991.
13. Fromm, M.E., Taylor, L.P., Walbot, V.: Stable transformation of maize after gene transfer by electroporation. - Nature 319: 791-793, 1986.
14. Horsch, R.B., Fry, J.E., Hoffman, N.L., Eichholtz, D., Rogers, S.G., Fraley, R.T.: A simple and general method for transferring genes into plants. - Science 227: 1229-1231, 1985.
15. Jefferson, R.A., Kavanagh, T.A., Bevan, M.W.: GUS fusions: β-glucuronidase as a sensitive and versatile gene fusion marker in higher plants. - EMBO J. 6: 3901-3907, 1987.
16. Jongedijk, E., Tigelaar, H., Van Roekel, J.S.C., Bres-Vloemans, S.A., Dekker, I., Van den Elzen, P.J.M., Cornelissen, B.J.C., Melchers, L.S.: Synergistic activity of chitinases and beta-1,3-glucanases enhances fungal resistance in transgenic tomato plants. - Euphytica 185: 173-180, 1995.
17. Laemmli, U.K.: Cleavage of structural proteins during the assembly of the head of bacteriophage T4. - Nature 277: 680-684, 1970.
18. Leah, R., Tommerup, H., Svendsen, I., Mundy, J.: Biochemical and molecular characterization of three barley seed proteins with antifungal properties. - J. biol. Chem. 266: 1564-1573, 1991.
19. Linthorst, H.J.M., Van Loon, L.C., Van Rossum, C.M.A., Mayer, A., Bol, J.F., van Roeckel, J.S.C., Meulenhoff, E.J.S., Cornelissen, B.J.C.: Analysis of acidic and basic chitinases from tobacco and petunia and their constitutive expression in transgenic tobacco. - Mol. Plant-Microbe Interact. 3: 252-258, 1990.
20. Liu, D., Raghothama, K.G., Hasegawa, P.M., Bressan, R.A.: Osmotin overexpression in potato delays development of disease symptoms. - Proc. nat. Acad. Sci. USA 91: 1888-1892, 1994.
21. Liu, D., Rhodes, D., D'Urzo, M.P., Xu, Y., Narasimhan, M.L., Hasegawa, P.M., Bressan, R.A., Abad, L.: In vivo and in vitro activity of truncated osmotin that is secreted into the extracellular matrix. - Plant Sci. 121: 123-131, 1996.
22. Lorito, M., Woo, S.L., Fernandez, I.G., Colucci, G., Harman, G.E., Pintor-Toro, J.A., Filippone, E., Muccifora, S., Lawrence, C.B., Zoina, A., Tuzun, S., Scala, F.: Genes from mycoparasitic fungi as a source for improving plant resistance to fungal pathogens. - Proc. nat. Acad. Sci. USA 95: 7860-7865, 1998.
23. Murray, M.G., Thompson, W.F.: Rapid isolation of high molecular weight plant DNA. - Nucl. Acids Res. 8: 4321-4325, 1980.
24. Parent, J.G., Asselin, A.: Detection of pathogenesis-related proteins (PR or b) and of other proteins in the intercellular fluid of hypersensitive plants infected with tobacco mosaic virus. - Can. J. Bot. 62: 564-569, 1984.
25. Punja, Z.K.: Genetic engineering of plants to enhance resistance to fungal pathogens - a review of progress and future prospects. - Can. J. Plant Pathol. 23: 216-235, 2001.
26. Roberts, W.K., Selitrennikoff, C.P.: Zeamatin, an antifungal protein from maize with membrane permeabilizing activity. - J. gen. Microbiol. 136: 1771-1778, 1990.
27. Rogers, S.G., Horsch, R.B., Fraley, R.T.: Gene transfer in plants: Production of transformed plants using Ti-plasmid vectors. - Methods Enzymol. 118: 627-640, 1986.
28. Sambrook, J., Fritsch, E.F., Maniatis, T.: Molecular Cloning: A Laboratory Manual. - Cold Spring Harbor Laboratory Press, Cold Spring Harbor - New York 1989.
29. Swegle, M., Kramer, K.J., Muthukrishnan, S.: Properties of barley seed chitinases and release of embryo-associated isoforms during early stages of imbibition. - Plant Physiol. 99: 1009-1014, 1992.
30. Terras, F.R.G., Schoofs, H., De Bolle, M.F.C., Van Leuven, F., Rees, S.B., Vanderleyden, J., Cammue, B.P.A., Broekaert, W.F.: Analysis of two novel classes of plant antifungal proteins from radish (Raphanus sativus L.) seeds. - J. biol. Chem. 267: 15301-15309, 1992.
31. Velazhahan, R., Cole, K.C., Anuratha, C.S., Muthukrishnan, S.: Induction of thaumatin-like proteins (TLPs) in Rhizoctonia solani-infected rice and characterization of two new cDNA clones. - Physiol. Plant. 102: 21-28, 1998.
32. Velazhahan, R., Datta, S.K., Muthukrishnan, S.: The PR-5 family: thaumatin-like proteins. - In: Datta, S.K., Muthukrishnan, S. (ed.): Pathogenesis-Related Proteins in Plants. Pp. 107-129. CRC Press, Boca Raton 1999.
33. Velazhahan, R., Jayaraj, J., Jeoung, J.M., Liang, G.H.. Muthukrishnan, S.: Purification and characterization of an antifungal thaumatin-like protein from sorghum (Sorghum bicolor) leaves. - J. Plant Dis. Protect. 109: 452-461, 2002.
34. Velazhahan, R., Jayaraj, J., Liang, G.H., Muthukrishnan, S.: Partial purification and N-terminal amino acid sequencing of a β-1,3-glucanase from sorghum (Sorghum bicolor) leaves. - Biol. Plant. 46: 29-33, 2003.
35. Velazhahan, R., Radhajeyalakshmi, R., Thangavelu, R., Muthukrishnan, S.: An antifungal protein purified from pearl millet seeds shows sequence homology to lipid transfer proteins. - Biol. Plant. 44: 417-421, 2001.
36. Velazhahan, R., Samiyappan, R., Vidhyasekaran, P.: Purification of an elicitor-inducible antifungal chitinase from suspension-cultured rice cells. - Phytoparasitica 28: 131-139, 2000.
37. Vigers, A.J., Roberts, W.K., Selitrennikoff, C.P.: A new family of plant antifungal proteins. - Mol. Plant-Microbe Interact. 4: 315-323, 1991.
38. Vigers, A.J., Wiedemann, S., Roberts, W.K., Legrand, M., Selitrennikoff, C.P., Fritig, B.: Thaumatin-like pathogenesis-related proteins are antifungal. - Plant Sci. 83: 155-161, 1992.
39. Winston, S., Fuller, S., Hurrel, J.: Current Protocols in Molecular Biology. - John Wiley and Sons, New York 1987.
40. Ye, X.Y., Wang, H.X., Ng, T.B.: First chromatographic isolation of an antifungal thaumatin-like protein from French bean legumes and demonstration of its antifungal activity. - Biochem. biophys. Res. Commun. 263: 130-134, 1999.
41. Zhu, Q., Maher, E.A., Masoud, S., Dixon, R.A., Lamb, C.J.: Enhanced protection against fungal attack by constitutive coexpression of chitinase and glucanase genes in transgenic tobacco. - Biotechnology 12: 807-812, 1994.