Detection of low-copy-number genomic DNA sequences in individual bacterial cells by using peptide nucleic acid-assisted rolling-circle amplification and fluorescence in situ hybridization

Applied and Environmental Microbiology

Volumn 73, Issue 7, 2007, Pages 2324-2328

  Smolina, Irina ^a   Lee, Charles ^b   Frank Kamenetskii, Maxim ^a  

^a Boston University   (United States)

^b BRIGHAM AND WOMEN S HOSPITAL   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

BACTERIA; CELLS; FLUORESCENCE; NUCLEIC ACIDS; OLIGONUCLEOTIDES; PEPTIDES;

AMPLICON; CIRCULAR OLIGONUCLEOTIDE; DECORATOR PROBES; IN SITU DETECTION;

DNA SEQUENCES;

FLUORESCENT DYE; GENOMIC DNA; OLIGONUCLEOTIDE; PEPTIDE NUCLEIC ACID;

AMPLIFICATION; BACTERIUM; CYTOLOGY; DETECTION METHOD; DNA FINGERPRINTING; FLUORESCENCE; GENOME; HYBRIDIZATION; IN SITU TEST; NUCLEIC ACID ANALYSIS; PEPTIDE;

AMPLICON; ARTICLE; BACILLUS SUBTILIS; BACTERIAL CELL; BACTERIAL GENOME; BACTERIUM DETECTION; DNA DETERMINATION; DNA SEQUENCE; ESCHERICHIA COLI; FLUORESCENCE; FLUORESCENCE IN SITU HYBRIDIZATION; FLUORESCENCE MICROSCOPY; IMAGE ANALYSIS; MOLECULAR GENETICS; NONHUMAN; ROLLING CIRCLE AMPLIFICATION; SEQUENCE ANALYSIS; STREPTOCOCCUS MUTANS;

BACILLUS SUBTILIS; DNA, BACTERIAL; ESCHERICHIA COLI; GENE DOSAGE; IN SITU HYBRIDIZATION, FLUORESCENCE; NUCLEIC ACID AMPLIFICATION TECHNIQUES; PEPTIDE NUCLEIC ACIDS; SEQUENCE ANALYSIS, DNA;

BACTERIA (MICROORGANISMS);

EID: 34247530295     PISSN: 00992240     EISSN: None     Source Type: Journal    
DOI: 10.1128/AEM.02038-06     Document Type: Article

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