Inhibitory effects of N-acetylcysteine on the functional responses of human eosinophils in vitro

Clinical and Experimental Allergy

Volumn 37, Issue 5, 2007, Pages 714-722

  Martinez Losa, M ^a   Cortijo, J ^a,b   Juan, G ^a,b   O'Connor, J E ^c   Sanz, M J ^a   Santangelo, F ^a   Morcillo, E J ^a,d,e  

^a UNIVERSITY OF VALENCIA   (Spain)

^b HOSPITAL GENERAL UNIVERSITARIO DE VALENCIA   (Spain)

^c CENTRO DE INVESTIGACIÓN PRÍNCIPE FELIPE   (Spain)

^d HOSPITAL CLÍNICO UNIVERSITARIO   (Spain)

^e UNIVERSITY OF VALENCIA   (Spain)

Author keywords

Eosinophil cationic protein; Human eosinophils; N acetylcysteine; p47 phox p67phox; Reactive oxygen species

Indexed keywords

ACETYLCYSTEINE; CALCIUM; EOSINOPHIL CATIONIC PROTEIN; EOTAXIN; FORMYLMETHIONYLLEUCYLPHENYLALANINE; GLUTATHIONE TRANSFERASE; HOX PROTEIN; HYDROGEN PEROXIDE; INTERLEUKIN 5; PROTEIN P47; PROTEIN P67; REACTIVE OXYGEN METABOLITE;

ALLERGY; ARTICLE; BLOOD; CALCIUM CELL LEVEL; CALCIUM SIGNALING; CELL FUNCTION; CELL ISOLATION; CELL MEMBRANE; CHEMOLUMINESCENCE; CONCENTRATION RESPONSE; CONTROLLED STUDY; DONOR; DRUG EFFECT; DRUG MECHANISM; EOSINOPHIL; EXTRACELLULAR SPACE; FLOW CYTOMETRY; FLUOROMETRY; GENE TRANSLOCATION; HUMAN; HUMAN CELL; IC 50; IN VITRO STUDY; INFLAMMATION; INHIBITION KINETICS; INTRACELLULAR SPACE; PRIORITY JOURNAL; PROTEIN LOCALIZATION; PROTEIN SECRETION; RADIOIMMUNOASSAY; SEPARATION TECHNIQUE; WESTERN BLOTTING;

ACETYLCYSTEINE; CALCIUM; CELL DEATH; CHEMOKINES, CC; EOSINOPHIL CATIONIC PROTEIN; EOSINOPHILS; FREE RADICAL SCAVENGERS; GLUTATHIONE; HUMANS; LUMINESCENCE; N-FORMYLMETHIONINE LEUCYL-PHENYLALANINE; NADPH OXIDASE; PHOSPHOPROTEINS; REACTIVE OXYGEN SPECIES; TRANSLOCATION, GENETIC;

EID: 34247385560     PISSN: 09547894     EISSN: 13652222     Source Type: Journal    
DOI: 10.1111/j.1365-2222.2007.02694.x     Document Type: Article

References (54)

1. Rahman I, Biswas SK, Kode A. Oxidant and antioxidant balance in the airways and airway diseases. Eur J Pharmacol 2006 533: 222 39.
2. Mak JC, Chan-Yeung MM. Reactive oxidant species in asthma. Curr Opin Pulm Med 2006 12: 7 11.
3. Morcillo EJ, Estrela J, Cortijo J. Oxidative stress and pulmonary inflammation: pharmacological intervention with antioxidants. Pharmacol Res 1999 40: 393 404.
4. Kay AB. The role of eosinophils in the pathogenesis of asthma. Trends Mol Med 2005 11: 148 52.
5. Lee JJ, Lee NA. Eosinophil degranulation: an evolutionary vestige or a universally destructive effector function? Clin Exp Allergy 2005 35: 986 94.
6. Rothenberg ME, Hogan SP. The eosinophil. Annu Rev Immunol 2006 24: 147 74.
7. Wedi B, Straede J, Wieland B, Kapp A. Eosinophil apoptosis is mediated by stimulators of cellular oxidative metabolisms and inhibited by antioxidants: involvement of a thiol-sensitive redox regulation in eosinophil cell death. Blood 1999 94: 2365 73.
8. Someya A, Nishijima K, Nunoi H, Irie S, Nagaoka I. Study on the superoxide-producing enzyme of eosinophils and neutrophils - comparison of the NADPH oxidase components. Arch Biochem Biophys 1997 345: 207 13.
9. Cortijo J, Marti-Cabrera M, de la Asuncion JG et al. Contraction of human airways by oxidative stress protection by N-acetylcysteine. Free Radic Biol Med 1999 27: 392 400.
10. Aitio M-L. N-acetylcysteine - passe-partout or much ado about nothing? Br J Clin Pharmacol 2005 61: 5 15.
11. Blesa S, Cortijo J, Mata M et al. Oral N-acetylcysteine attenuates the rat pulmonary inflammatory response to antigen. Eur Respir J 2003 21: 394 400.
12. Demedts M, Behr J, Buhl R et al. High-dose acetylcysteine in idiopathic pulmonary fibrosis. N Engl J Med 2005 353: 2229 42.
13. Appelboam AV, Dargan PI, Knighton J. Fatal anaphylactoid reaction to N-acetylcysteine: caution in patients with asthma. Emerg Med J 2002 19: 594 5.
14. Le Y, Murphy PM, Wang JM. Formyl-peptide receptors revisited. Trends Immunol 2002 23: 541 8.
15. Barnes PJ. New drugs for asthma. Nat Rev Drug Discov 2004 3: 831 44.
16. Koh GC, Shek LP, Goh DY, Van Bever H, Koh DS. Eosinophil cationic protein: is it useful in asthma? A systematic review. Respir Med 2006 101: 696 705.
17. Morcillo EJ, Cortijo J, Martinez-Losa M, Blesa S, Mata M, Santangelo F. Effects of N-acetylcysteine on functional responses of human eosinophils in vitro (abstract). Am J Respir Crit Care Med 2001 163: A723.
18. Boyum A. Isolation of mononuclear cells and granulocytes from human blood. Isolation of mononuclear cells by one centrifugation, and of granulocytes by combining centrifugation and sedimentation at 1 g. Scand J Clin Lab Invest 1968 97 (Suppl): 77 89.
19. Cortijo J, Villagrasa V, Pons R et al. Bronchodilator and anti-inflammatory activities of glaucine: in vitro studies in human airway smooth muscle and polymorphonuclear leukocytes. Br J Pharmacol 1999 127: 1641 51.
20. Mata M, Sarria B, Buenestado A, Cortijo J, Cerda M, Morcillo EJ. Phosphodiesterase 4 inhibition decreases MUC5AC expression induced by epidermal growth factor in human airway epithelial cells. Thorax 2005 60: 144 52.
21. Hatzelmann A, Tenor H, Schudt C. Differential effects of non-selective and selective phosphodiesterase inhibitors on human eosinophil functions. Br J Pharmacol 1995 114: 821 31.
22. Woerly G, Decot V, Loiseau S et al. CD28 and secretory immunoglobulin A-dependent activation of eosinophils: inhibition of mediator release by the anti-allergic drug, suplatast tosilate. Clin Exp Allergy 2004 34: 1379 87.
23. Robinson JP, Carter WO, Narayanan PK. Oxidative product formation analysis by flow cytometry. Methods Cell Biol 1994 41: 437 47.
24. Cortijo J, Villagrasa V, Navarrete C et al. Effects of SCA40 on human isolated bronchus and human polymorphonuclear leukocytes: comparison with rolipram, SKF94120 and levcromakalim. Br J Pharmacol 1996 119: 99 106.
25. O'Connor JE, Kimler BF, Morgan MC, Tempas KJ. A flow cytometric assay for intracellular nonprotein thiols using mercury orange. Cytometry 1988 9: 529 32.
26. Bowes TJ, Gupta RS. Induction of mitochondrial fusion by cysteine-alkylators ethacrynic acid and N-ethylmaleimide. J Cell Physiol 2005 202: 796 804.
27. Clark RA, Volpp BD, Leidal KG, Nauseef WM. Two cytosolic components of the human neutrophil respiratory burst oxidase translocate to the plasma membrane during cell activation. J Clin Invest 1990 85: 714 21.
28. Kharazmi A, Nielsen H, Schiotz PO. N-acetylcysteine inhibits human neutrophil and monocyte chemotaxis and oxidative metabolism. Int J Immunopharmacol 1988 10: 39 46.
29. Witko-Sarsat V, Gausson V, Nguyen AT et al. AOPP-induced activation of human neutrophil and monocyte oxidative metabolism: a potential target for N-acetylcysteine treatment in dialysis patients. Kidney Int 2003 64: 82 91.
30. Wymann MP, Kernen P, Von Tscharner V, Tai PC, Spry CJ, Baggiolini M. Activation of the respiratory burst in eosinophil leucocytes - a transduction sequence decoupled from cytosolic Ca2+rise. Eur J Clin Invest 1995 25: 25 31.
31. Stolarek R, Bialasiewicz P, Nowak D. N-acetylcysteine effect on the luminol-dependent chemiluminescence pattern of reactive oxygen species generation by human polymorphonuclear leukocytes. Pulm Pharmacol Ther 2002 15: 385 92.
32. Villagrasa V, Cortijo J, Marti-Cabrera M et al. Inhibitory effects of N-acetylcysteine on superoxide anion generation in human polymorphonuclear leukocytes. J Pharm Pharmacol 1997 49: 525 29.
33. Tirouvanziam R, Conrad CK, Bottiglieri T, Herzenberg LA, Moss RB, Herzenberg LA. High-dose oral N-acetylcysteine, a glutathione prodrug, modulates inflammation in cystic fibrosis. Proc Natl Acad Sci USA 2006 103: 4628 33.
34. Hua J, Hasebe T, Someya A, Nakamura S, Sugimoto K, Nagaoka I. Evaluation of the expression of NADPH oxidase components during maturation of HL-60 clone 15 cells to eosinophilic lineage. Inflamm Res 2001 50: 156 67.
35. Kitaoka N, Liu G, Masuoka N, Yamashita K, Manabe M, Kodama H. Effect of sulfur amino acids on stimulus-induced superoxide generation and translocation of p47phox and p67phox to cell membrane in human neutrophils and the scavenging of free radical. Clin Chim Acta 2005 353: 109 16.
36. Abu-Ghazaleh RI, Dunnette SL, Loegering DA et al. Eosinophil granule proteins in peripheral blood granulocytes. J Leukoc Biol 1992 52: 611 8.
37. Tjabringa GS, Ninaber DK, Drijfhout JW, Rabe KF, Hiemstra PS. Human cathelicidin LL-37 is a chemoattractant for eosinophils and neutrophils that acts via formyl-peptide receptors. Int Arch Allergy Immunol 2006 140: 103 12.
38. Nagy AM, Vanderbist F, Parij N, Maes P, Fondu P, Neve J. Effect of the mucoactive drug nacystelyn on the respiratory burst of human blood polymorphonuclear neutrophils. Pulm Pharmacol Ther 1997 10: 287 92.
39. Dent G, Rabe KF, Magnussen H. Augmentation of human neutrophil and alveolar macrophage LTB4 production by N-acetylcysteine: role of hydrogen peroxide. Br J Pharmacol 1997 122: 758 64.
40. Gressier B, Cabanis A, Lebegue S et al. Comparison of in vitro effects of two thiol-containing drugs on human neutrophils hydrogen peroxide production. Methods Find Exp Clin Pharmacol 1993 15: 101 5.
41. Paulsen O, Forsgren A. Effects of N-acetylcysteine on human polymorphonuclear leukocytes. Apmis 1989 97: 115 9.
42. Cotgreave IA. N-acetylcysteine: pharmacological considerations and experimental and clinical applications. Adv Pharmacol 1997 38: 205 27.
43. Yamauchi A, Ueda N, Hanafusa S, Yamashita E, Kihara M, Naito S. Tissue distribution of and species differences in deacetylation of N-acetyl-l-cysteine and immunohistochemical localization of acylase I in the primate kidney. J Pharm Pharmacol 2002 54: 205 12.
44. Uttamsingh V, Baggs RB, Krenitsky DM, Anders MW. Immunohistochemical localization of the acylases that catalyze the deacetylation of N-acetyl-l-cysteine and haloalkene-derived mercapturates. Drug Metab Dispos 2000 28: 625 32.
45. Hatzelmann A, Ullrich V. Regulation of 5-lipoxygenase activity by the glutathione status in human polymorphonuclear leukocytes. Eur J Biochem 1987 169: 175 84.
46. Drost E, Lannan S, Bridgeman MM et al. Lack of effect of N-acetylcysteine on the release of oxygen radicals from neutrophils and alveolar macrophages. Eur Respir J 1991 4: 723 29.
47. Borgstrom L, Kagedal B, Paulsen O. Pharmacokinetics of N-acetylcysteine in man. Eur J Clin Pharmacol 1986 31: 217 22.
48. Babior BM. NADPH oxidase: an update. Blood 1999 93: 1464 76.
49. Sedgwick JB, Vrtis RF, Gourley MF, Busse WW. Stimulus-dependent differences in superoxide anion generation by normal human eosinophils and neutrophils. J Allergy Clin Immunol 1988 81: 876 83.
50. Eklund A, Eriksson O, Hakansson L et al. Oral N-acetylcysteine reduces selected humoral markers of inflammatory cell activity in BAL fluid from healthy smokers: correlation to effects on cellular variables. Eur Respir J 1988 1: 832 8.
51. van Overveld FJ, Demkow U, Gorecka D, de Backer WA, Zielinski J. New developments in the treatment of COPD: comparing the effects of inhaled corticosteroids and N-acetylcysteine. J Physiol Pharmacol 2005 56 (Suppl. 4): 135 42.
52. Lee YC, Lee KS, Park SJ et al. Blockade of airway hyperresponsiveness and inflammation in a murine model of asthma by a prodrug of cysteine, l-2-oxothiazolidine-4-carboxylic acid. Faseb J 2004 18: 1917 9.
53. Pazdrak K, Olszewska-Pazdrak B, Stafford S, Garofalo RP, Alam R. Lyn, Jak2, and Raf-1 kinases are critical for the antiapoptotic effect of interleukin 5, whereas only Raf-1 kinase is essential for eosinophil activation and degranulation. J Exp Med 1998 188: 421 9.
54. Lappas M, Permezel M, Rice GE. N-Acetyl-cysteine inhibits phospholipid metabolism, proinflammatory cytokine release, protease activity, and nuclear factor-kappaB deoxyribonucleic acid-binding activity in human fetal membranes in vitro. J Clin Endocrinol Metab 2003 88: 1723 9.